Anti-PSD93抗体(ab2930)
Key features and details
- Rabbit polyclonal to PSD93
- Suitable for: IHC-Fr, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-PSD93抗体
参阅全部 PSD93 一抗 -
描述
兔多克隆抗体to PSD93 -
宿主
Rabbit -
特异性
Detects Post Synaptic Density 93 (PSD 93) from rat tissues. This antibody does not detect other synapse-associated protein family members. -
经测试应用
适用于: IHC-Fr, WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Rat PSD93 aa 352-366.
Sequence:NKLCDKPASPRHYSP
(Peptide available asab5840) -
阳性对照
- IHC-Fr: Rat cerebellum. WB: SH-SY5Y and Neuro-2a cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
Primary antibody说明
Post Synaptic Density 93 (PSD 93), also known as chapsyn-110, is one of a family of plasma membrane-associated proteins found in synaptic junctions. PSD 93 is unique among family members in its expression in Purkinje neuron cell bodies and dendrites. PSD 93 has three ~90 amino acid repeats called PDZ domains, a single interior SH3 domain, and a carboxyl-terminal guanylate kinase homology (GuK) domain that is enzymatically inactive. It is hypothesized that PDZ-domain interactions play a role in receptor and channel clustering which contributes to neuronal plasticity. PSD 93 is believed to participate in the clustering of certain proteins, including N-methyl-D-aspartate (NMDA) receptors and shaker-type potassium channels at the synaptic membrane. There are two principal modes of interaction between PSD 93 and other proteins. NMDA receptors and shaker-type potassium channels both share C-terminal sequence homology consisting of a threonine/serine-X-valine-COOH (T/SXV) motif. Other neuronal proteins that share this motif (beta 1 adrenergic receptor, some serotonin receptors, some sodium channel subunits, and additional potassium channel subunits) may interact with PSD 93 by binding to its PDZ domains. Neuronal nitric oxide synthase (nNOS), which lacks the T/SXV motif but which has its own PDZ domain, has been shown to associate with PSD 93 in vitro through a pseudo-homotypic PDZ-PDZ interaction. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-Fr |
Use a concentration of 5 µg/ml.
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| WB |
Use a concentration of 3 µg/ml. Detects a band of approximately 110 kDa.Can be blocked with PSD93 peptide (ab5840).
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| ICC/IF |
Use a concentration of 1 µg/ml.
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| 说明 |
|---|
|
IHC-Fr
Use a concentration of 5 µg/ml. |
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WB
Use a concentration of 3 µg/ml. Detects a band of approximately 110 kDa.Can be blocked with PSD93 peptide (ab5840). |
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ICC/IF
Use a concentration of 1 µg/ml. |
靶标
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功能
Required for perception of chronic pain through NMDA receptor signaling. Regulates surface expression of NMDA receptors in dorsal horn neurons of the spinal cord. Interacts with the cytoplasmic tail of NMDA receptor subunits as well as inward rectifying potassium channels. Involved in regulation of synaptic stability at cholinergic synapses. Part of the postsynaptic protein scaffold of excitatory synapses. -
序列相似性
Belongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 3 PDZ (DHR) domains.
Contains 1 SH3 domain. -
结构域
An N-terminally truncated L27 domain is predicted in isoform 2 at positions 1 through 27. -
翻译后修饰
Palmitoylation of isoform 1 is not required for targeting to postsynaptic density. -
细胞定位
Cell membrane. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell junction, synapse. Membrane. Cell projection, axon. Concentrated in soma and postsynaptic density of a subset of neurons. - Information by UniProt
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数据库链接
- Entrez Gene: 1740 Human
- Entrez Gene: 23859 Mouse
- Entrez Gene: 64053 Rat
- Omim: 603583 Human
- SwissProt: Q15700 Human
- SwissProt: Q91XM9 Mouse
- SwissProt: Q63622 Rat
- Unigene: 367656 Human
see all -
别名
- Channel associated protein of synapse 110 antibody
- Channel associated protein of synapses 110kD antibody
- Channel-associated protein of synapse-110 antibody
see all
图片
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Immunohistochemistry (Frozen sections) - Anti-PSD93 antibody (ab2930)
Immunolocalization of PSD-93 in rat cerebellum using ab2930.
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Western blot - Anti-PSD93 antibody (ab2930)All lanes : Anti-PSD93 antibody (ab2930) at 3 µg/ml
Lane 1 : SH-SY5Y (human neuroblastoma cell line) whole cell lysate
Lane 2 : Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, HRP conjugate at 1/4000 dilution -
Immunocytochemistry/ Immunofluorescence - Anti-PSD93 antibody (ab2930)ICC/IF image of ab2930 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2930, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
数据表及文件
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Datasheet download
文献 (5)
ab2930 被引用在 5 文献中.
- Zhuang RJ et al. MicroRNA-23a depletion promotes apoptosis of ovarian cancer stem cell and inhibits cell migration by targeting DLG2. Cancer Biol Ther 20:897-911 (2019). PubMed: 30862230
- Mahaman YAR et al. Moringa Oleifera Alleviates Homocysteine-Induced Alzheimer's Disease-Like Pathology and Cognitive Impairments. J Alzheimers Dis 63:1141-1159 (2018). PubMed: 29710724
- Li XH et al. Glycation exacerbates the neuronal toxicity of ß-amyloid. Cell Death Dis 4:e673 (2013). Mouse . PubMed: 23764854
- Sun Q & Turrigiano GG PSD-95 and PSD-93 Play Critical But Distinct Roles in Synaptic Scaling Up and Down. J Neurosci 31:6800-8 (2011). ICC/IF ; Rat . PubMed: 21543610
- Sahab ZJ et al. Tumor Suppressor RARRES1 Regulates DLG2, PP2A, VCP, EB1, and Ankrd26. J Cancer 1:14-22 (2010). WB ; Human . PubMed: 20842219
