Anti-PSD93抗体(ab2930)
Key features and details
- Rabbit polyclonal to PSD93
- Suitable for: IHC-Fr, WB, ICC/IF
- Reacts with: Rat
- Isotype: IgG
概述
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产品名称
Anti-PSD93抗体
参阅全部 PSD93 一抗 -
描述
兔多克隆抗体to PSD93 -
宿主
Rabbit -
特异性
Detects Post Synaptic Density 93 (PSD 93) from rat tissues. This antibody does not detect other synapse-associated protein family members. -
经测试应用
适用于: IHC-Fr, WB, ICC/IFmore details -
种属反应性
与反应: Rat
预测可用于: Mouse, Human
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免疫原
Synthetic peptide corresponding to Rat PSD93 aa 352-366.
Sequence:NKLCDKPASPRHYSP
(Peptide available asab5840) -
常规说明
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
Primary antibody说明
Post Synaptic Density 93 (PSD 93), also known as chapsyn-110, is one of a family of plasma membrane-associated proteins found in synaptic junctions. PSD 93 is unique among family members in its expression in Purkinje neuron cell bodies and dendrites. PSD 93 has three ~90 amino acid repeats called PDZ domains, a single interior SH3 domain, and a carboxyl-terminal guanylate kinase homology (GuK) domain that is enzymatically inactive. It is hypothesized that PDZ-domain interactions play a role in receptor and channel clustering which contributes to neuronal plasticity. PSD 93 is believed to participate in the clustering of certain proteins, including N-methyl-D-aspartate (NMDA) receptors and shaker-type potassium channels at the synaptic membrane. There are two principal modes of interaction between PSD 93 and other proteins. NMDA receptors and shaker-type potassium channels both share C-terminal sequence homology consisting of a threonine/serine-X-valine-COOH (T/SXV) motif. Other neuronal proteins that share this motif (beta 1 adrenergic receptor, some serotonin receptors, some sodium channel subunits, and additional potassium channel subunits) may interact with PSD 93 by binding to its PDZ domains. Neuronal nitric oxide synthase (nNOS), which lacks the T/SXV motif but which has its own PDZ domain, has been shown to associate with PSD 93 in vitro through a pseudo-homotypic PDZ-PDZ interaction. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
应用
Our Abpromise guarantee covers the use of ab2930 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-Fr | Use a concentration of 5 µg/ml. | |
| WB | Use a concentration of 3 µg/ml. Detects a band of approximately 110 kDa.Can be blocked with PSD93 peptide (ab5840). | |
| ICC/IF | Use a concentration of 1 µg/ml. |
靶标
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功能
Required for perception of chronic pain through NMDA receptor signaling. Regulates surface expression of NMDA receptors in dorsal horn neurons of the spinal cord. Interacts with the cytoplasmic tail of NMDA receptor subunits as well as inward rectifying potassium channels. Involved in regulation of synaptic stability at cholinergic synapses. Part of the postsynaptic protein scaffold of excitatory synapses. -
序列相似性
Belongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 3 PDZ (DHR) domains.
Contains 1 SH3 domain. -
结构域
An N-terminally truncated L27 domain is predicted in isoform 2 at positions 1 through 27. -
翻译后修饰
Palmitoylation of isoform 1 is not required for targeting to postsynaptic density. -
细胞定位
Cell membrane. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell junction, synapse. Membrane. Cell projection, axon. Concentrated in soma and postsynaptic density of a subset of neurons. - Information by UniProt
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数据库链接
- Entrez Gene: 1740 Human
- Entrez Gene: 23859 Mouse
- Entrez Gene: 64053 Rat
- Omim: 603583 Human
- SwissProt: Q15700 Human
- SwissProt: Q91XM9 Mouse
- SwissProt: Q63622 Rat
- Unigene: 367656 Human
see all -
别名
- Channel associated protein of synapse 110 antibody
- Channel associated protein of synapses 110kD antibody
- Channel-associated protein of synapse-110 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-PSD93 antibody (ab2930)Immunolocalization of PSD-93 in rat cerebellum using ab2930.
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Western blot - Anti-PSD93 antibody (ab2930)Western blot of PSD-93 on rat brain extract using ab2930.
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Immunocytochemistry/ Immunofluorescence - Anti-PSD93 antibody (ab2930)ICC/IF image of ab2930 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2930, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
数据表及文件
文献 (4)
ab2930 被引用在 4 文献中.
- Mahaman YAR et al. Moringa Oleifera Alleviates Homocysteine-Induced Alzheimer's Disease-Like Pathology and Cognitive Impairments. J Alzheimers Dis 63:1141-1159 (2018). PubMed: 29710724
- Li XH et al. Glycation exacerbates the neuronal toxicity of ß-amyloid. Cell Death Dis 4:e673 (2013). Mouse . PubMed: 23764854
- Sun Q & Turrigiano GG PSD-95 and PSD-93 Play Critical But Distinct Roles in Synaptic Scaling Up and Down. J Neurosci 31:6800-8 (2011). ICC/IF ; Rat . PubMed: 21543610
- Sahab ZJ et al. Tumor Suppressor RARRES1 Regulates DLG2, PP2A, VCP, EB1, and Ankrd26. J Cancer 1:14-22 (2010). WB ; Human . PubMed: 20842219
