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Correct sample preparation is a key step for optimal performance for ELISAs and activity assays.
Our recommendations for tissue and cultured cell line preparation for ELISAs and activity assays will help you get the best from your assays.
Buffer selection for lysis of tissues and cells:
For cellular assays:
150 mM sodium chloride
1.0% NP-40 or Triton X-100
0.5% sodium deoxycholate
0.1% SDS (sodium dodecyl sulphate)
50 mM Tris pH 8.0
Or purchase our 10X RIPA Buffer (ab156034).