概述

  • 产品名称
    Anti-Protein phosphatase 1 gamma 2抗体[EPR8934]
    参阅全部 Protein phosphatase 1 gamma 2 一抗
  • 描述
    兔单克隆抗体[EPR8934] to蛋白phosphatase 1 gamma 2
  • 宿主
    Rabbit
  • 经测试应用
    适用于: IP, WBmore details
    不适用于: Flow Cyt,ICC/IF or IHC-P
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human Protein phosphatase 1 gamma 2 aa 250-350 (C terminal). The exact sequence is proprietary.

  • 阳性对照
    • 293T, HeLa, SKBR3 and T47D cell lysates.
  • 常规说明

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab134947 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP 1/10 - 1/100.
WB 1/1000 - 1/10000. Predicted molecular weight: 39 kDa.
  • 应用说明
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • 靶标

    • 功能
      Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Dephosphorylates RPS6KB1. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca(2+)/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase.
    • 序列相似性
      Belongs to the PPP phosphatase family. PP-1 subfamily.
    • 翻译后修饰
      Phosphorylated by NEK2.
    • 细胞定位
      Cytoplasm. Nucleus. Nucleus > nucleolus. Nucleus > nucleoplasm. Nucleus speckle. Chromosome > centromere > kinetochore. Cleavage furrow. Midbody. Mitochondrion. Colocalizes with SPZ1 in the nucleus (By similarity). Colocalizes with URI1 at mitochondrion. Rapidly exchanges between the nucleolar, nucleoplasmic and cytoplasmic compartments. Highly mobile in cells and can be relocalized through interaction with targeting subunits. In the presence of PPP1R8 relocalizes from the nucleolus to nuclear speckles. Shows a dynamic targeting to specific sites throughout the cell cycle. Highly concentrated in nucleoli of interphase cells and localizes at kinetochores early in mitosis. Relocalization to chromosome-containing regions occurs at the transition from early to late anaphase. Also accumulates at the cleavage furrow and midbody by telophase.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ppp1cc-a antibody
      • PP 1G antibody
      • PP-1G antibody
      • PP1 antibody
      • PP1C antibody
      • PP1G antibody
      • PP1G_HUMAN antibody
      • PPP1CC antibody
      • PPP1G antibody
      • Protein phosphatase 1 catalytic subunit gamma isoform antibody
      • Protein phosphatase 1, catalytic subunit, gamma isozyme antibody
      • Protein phosphatase 1C catalytic subunit antibody
      • Serine/threonine phosphatase 1 gamma antibody
      • Serine/threonine protein phosphatase PP1 gamma catalytic subunit antibody
      • Serine/threonine-protein phosphatase PP1-gamma catalytic subunit antibody
      see all

    图片

    • All lanes : Anti-Protein phosphatase 1 gamma 2 antibody [EPR8934] (ab134947) at 1/1000 dilution

      Lane 1 : T47D cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : SKBR3 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 39 kDa

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    文献

    This product has been referenced in:
    • Cai J  et al. Simultaneous overactivation of Wnt/ß-catenin and TGFß signalling by miR-128-3p confers chemoresistance-associated metastasis in NSCLC. Nat Commun 8:15870 (2017). Read more (PubMed: 28627514) »
    • Garbacz WG  et al. Hepatic Overexpression of CD36 Improves Glycogen Homeostasis and Attenuates High-Fat Diet-Induced Hepatic Steatosis and Insulin Resistance. Mol Cell Biol 36:2715-2727 (2016). Read more (PubMed: 27528620) »
    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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