蛋白L Sepharose® (ab193261)
概述
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产品名称
蛋白L Sepharose® -
产品概述
Contents:
Supplied as a 50% slurry in 20% Ethanol, >5 mg Protein L/mL of Sepharose® beads.
Features:
Binding capacity of human IgG is greater than 10 mg/mL of gel; high flow rate; low falling off of rProtein L; pH stability 2-10.
These beads are for use in column purification. If used in batch purification, we recommend not exceeding 150 x g when centrifuging.
Store beads at 4°C.
The beads may be damaged above 40°C.
DO NOT FREEZE.
Wash beads 3 times with 3x bead volume of desired buffer before use.
Applications:
- Purification of monoclonal and polyclonal antibodies.
- Immunoprecipitation.
Sepharose is a registered trademark of GE Healthcare
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说明
This product is manufactured by BioVision, an Abcam company and was previously called 6531 Protein L Sepharose. 6531-25 is the same size as the 25 ml size of ab193261.
Protein L Sepharose® is prepared by covalently coupling recombinant Protein L (which contains five Ig light chain binding domains) to 6% cross-linked Sepharose® beads. The coupling technique is optimized to give high binding capacity. The capacity of IgG binding is generally greater than 10 mg of human IgG per mL of wet gel. Protein L binds to a wider range of antibody classes than Protein A or Protein G, including human, mouse and rat IgM, IgA, IgE, and IgD, as well as Fab and single chain variable fragments (scFv). Protein L binds only binds to antibodies that contain Κ light chains, which are found in most human and mouse antibodies.
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经测试应用
适用于: Purification, IPmore details
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 ml 5 ml 100 ml 25 ml Protein L Sepharose® 1 x 1ml 1 x 5ml 1 x 100ml 1 x 25ml -
研究领域
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应用
The Abpromise guarantee
Abpromise™承诺保证使用ab193261于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Purification |
Use at an assay dependent concentration.
Purification of monoclonal and polyclonal antibodies. |
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IP |
Use at an assay dependent concentration.
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说明 |
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Purification
Use at an assay dependent concentration. Purification of monoclonal and polyclonal antibodies. |
IP
Use at an assay dependent concentration. |
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
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