重组Anti-PRKAR1A+PRKAR1B抗体[EPR8491] (ab139695)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8491] to PRKAR1A+PRKAR1B
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
-
产品名称
Anti-PRKAR1A+PRKAR1B抗体[EPR8491]
参阅全部 PRKAR1A+PRKAR1B 一抗 -
描述
兔单克隆抗体[EPR8491] to PRKAR1A+PRKAR1B -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human PRKAR1A+PRKAR1B aa 200-300. The exact sequence is proprietary. Within PRKAR1A + PRKAR1B.
-
阳性对照
- WB: Recombinant Human PRKAR1A and PRKAR1B protein; Human, mouse, rat brain lysate, U-87 MG whole cell lysate; IHC-P: Mouse liver, human liver carcinoma, and rat kidney tissue sections.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at -20ºC. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR8491 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
-
Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab139695于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 43 kDa.
|
|
IHC-P |
1/300. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
The use of an HRP/AP polymerized secondary antibody is recommended. |
说明 |
---|
WB
1/1000 - 1/10000. Predicted molecular weight: 43 kDa. |
IHC-P
1/300. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. The use of an HRP/AP polymerized secondary antibody is recommended. |
图片
-
All lanes : Anti-PRKAR1A+PRKAR1B antibody [EPR8491] (ab139695) at 1/10000 dilution (Purified)
Lane 1 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysate
Lane 2 : Mouse brain lysate
Lane 3 : Rat brain lysate
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 43 kDaThe doublets should be PRKAR1A isoforms.
-
Anti-PRKAR1A+PRKAR1B antibody [EPR8491] (ab139695) at 1/10000 dilution (Purified) + Human brain lysate
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 43 kDaThe doublets should be PRKAR1A isoforms.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labeling PRKAR1A+PRKAR1B with Purified ab139695 at 1/300 dilution (1.29 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labeling PRKAR1A+PRKAR1B with Purified ab139695 at 1/300 dilution (1.29 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver carcinoma tissue sections labeling PRKAR1A+PRKAR1B with Purified ab139695 at 1/300 dilution (1.29 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
-
All lanes : Anti-PRKAR1A+PRKAR1B antibody [EPR8491] (ab139695) at 1/10000 dilution
Lane 1 : Recombinant human full length PRKAR1A protein
Lane 2 : Recombinant human full length PRKAR1B protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 43 kDaBuffer and concentration: 5% NFDM/TBST
Exposure time
Lane 1: 10 seconds
Lande 2: 40 seconds
Observed band
Lane 1: 51KDa
Lane 2: 52 KDa
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (5)
ab139695 被引用在 5 文献中.
- Zhang X et al. STK25 inhibits PKA signaling by phosphorylating PRKAR1A. Cell Rep 40:111203 (2022). PubMed: 35977512
- Liu Y et al. Loss of PKA regulatory subunit 1α aggravates cardiomyocyte necrosis and myocardial ischemia/reperfusion injury. J Biol Chem 297:100850 (2021). PubMed: 34087234
- Liu Y et al. PRKAR1A deficiency impedes hypertrophy and reduces heart size. Physiol Rep 8:e14405 (2020). PubMed: 32212257
- He J et al. Recurrent somatic mutations ofPRKAR1Ain isolated cardiac myxoma. Oncotarget 8:103968-103974 (2017). PubMed: 29262613
- Wang S et al. PRKAR1A is a functional tumor suppressor inhibiting ERK/Snail/E-cadherin pathway in lung adenocarcinoma. Sci Rep 6:39630 (2016). IHC-P ; Human . PubMed: 27995993