蛋白A/G Sepharose® (ab193262)
概述
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产品名称
蛋白A/G Sepharose® -
产品概述
High binding capacity Protein A/G Sepharose (>20 mg IgG/mL). Minimal leaching of ligand. Suitable for column or batch purification of IgG.
Contents:
Supplied as a 50% slurry in 20% Ethanol; >1.5 mg Protein A/G per mL Sepharose® beads.
Features:
Binding capacity greater than 20 mg of wet gel; high flow rate; low falling off of rProtein A/G; pH stability 2-10.
These beads are for use in column purification. If used in batch purification, we recommend not exceeding 150 x g when centrifuging.
Store beads at 4°C.
The beads may be damaged above 40°C.
DO NOT FREEZE.
Wash beads 3 times with 3x bead volume of desired buffer before use.
Applications:
Purification of monoclonal and polyclonal antibodies.
Sepharose is a registered trademark of GE Healthcare
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说明
This product is manufactured by BioVision, an Abcam company and was previously called 6503 Protein A/G Sepharose. 6503-1 is the same size as the 1 ml size of ab193262.
Protein A/G Sepharose® is prepared by covalently coupling recombinant Protein A/G (contains five Ig-binding regions of protein A and three Ig-binding regions of protein G) to 6% cross-linked Sepharose® beads. The coupling was optimized to give a high binding capacity for IgG. The capacity of IgG binding could be greater than 20 mg of rabbit IgG per ml of wet gel.
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经测试应用
适用于: Purificationmore details
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 标识符 25 ml 1 ml 100 ml 5 ml Protein A/G Sepharose® — 1 x 1ml 1 x 100ml 1 x 5ml -
研究领域
相关产品
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应用
The Abpromise guarantee
Abpromise™承诺保证使用ab193262于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Purification |
Use at an assay dependent concentration.
Purification of monoclonal and polyclonal antibodies. |
说明 |
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Purification
Use at an assay dependent concentration. Purification of monoclonal and polyclonal antibodies. |
数据表及文件
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SDS download
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Datasheet download
文献 (26)
ab193262 被引用在 26 文献中.
- Yang N et al. Blockage of PPARγ T166 phosphorylation enhances the inducibility of beige adipocytes and improves metabolic dysfunctions. Cell Death Differ 30:766-778 (2023). PubMed: 36329235
- Luo X et al. Pathogenesis-related protein 1 suppresses oomycete pathogen by targeting against AMPK kinase complex. J Adv Res 43:13-26 (2023). PubMed: 36585103
- Haj-Shomaly J et al. T Cells Promote Metastasis by Regulating Extracellular Matrix Remodeling following Chemotherapy. Cancer Res 82:278-291 (2022). PubMed: 34666995
- Desjardins P et al. The WNK1 kinase regulates the stability of transcription factors during wound healing of human corneal epithelial cells. J Cell Physiol 237:2434-2450 (2022). PubMed: 35150137
- Yang M et al. MCTS1 promotes laryngeal squamous cell carcinoma cell growth via enhancing LARP7 stability. Clin Exp Pharmacol Physiol 49:652-660 (2022). PubMed: 35274760