蛋白A Agarose (High Affinity) (ab193255)
Key features and details
- Sample type: Ascites Fluid, Cell culture media, Cell culture supernatant, Serum
概述
-
产品名称
蛋白A Agarose (High Affinity) -
样品类型
Cell culture supernatant, Serum, Cell culture media, Ascites Fluid -
产品概述
Very high binding capacity (>35 mg IgG/mL). Minimal leaching of ligand. Suitable for column or batch purification of IgG, immunoprecipitation & ChIP (ab193255).
Contents:
Supplied as 50% slurry in 20% Ethanol.
Features:
High binding capacity = Binding of IgG ≥ 36 mg human or rabbit IgG/mL Protein A Agarose.
Minimal leaching of the ligand
Flow Rate Tested* = 2.89 mL/min.
*Test conditions: = Calculations based on the time required to pass 18 mL of water through 2 mL settled beads (column diameter 1.5 cm).
Maximum Flow Rate** = 1800 cm/hr; minimum leaching of recombinant Protein A.
**NOTE = the highest flow that beads withstand for 1 minute, without collapsing and the pressure reaching 1 MPa.
Usage = Reusable for up to 10 times without significant loss of binding capacity.
Store beads at 4°C.
The beads may be damaged above 40°C.
DO NOT FREEZE.
Wash beads 3 times with 3x bead volume of desired buffer before use.
Applications:
- Purification of monoclonal and polyclonal antibodies from culture media, serum, ascites fluid or hybridoma supernatants.
- Isolation of antibody/antigen complexes in immunoprecipitation experiments, since only the Fc region is involved in antibody binding and the Fab region is available for binding antigen. -
说明
This product is manufactured by BioVision, an Abcam company and was previously called 6520 Hi-Bind™ Protein A-Agarose. 6520-100 is the same size as the 100 ml size of ab193255.
Abcam's Protein A Agarose (High Affinity) beads are specially prepared for high IgG binding by covalently coupling recombinant Protein A to 6% cross-linked agarose beads, the most popular resin for protein affinity purification methods. Protein A is a genetically engineered protein containing five IgG-binding regions of native Protein A. The cell wall binding region, albumin binding region and other non-specific regions have been eliminated from the recombinant Protein A to ensure maximum specific IgG binding. The coupling technique is optimized to give a higher binding capacity for IgG & minimum leaching of recombinant Protein A compared to standard Protein A agarose beads. The IgG binding capacity of Protein A Agarose (High Affinity) is ≥ 36 mg human or rabbit IgG per mL of wet beads. The Protein A Agarose (High Affinity) beads display high chemical and physical stability as well as high flow rate, hydrophilicity and high gel strength. This product can be used for IgG purification and immunoprecipitation.
-
经测试应用
适用于: IP, Purificationmore details
性能
-
存放说明
Store at +4°C. Please refer to protocols. -
组件 100 ml 1 ml 5 ml 25 ml 1 ml Protein A-Agarose (High Affinity) 1 x 100ml 1 x 1ml 1 x 5ml 1 x 25ml 1 x 1ml -
研究领域
相关产品
-
Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab193255于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IP |
Use at an assay dependent concentration.
Isolation of antibody/antigen complexes in immunoprecipitation experiments, since only the Fc region is involved in antibody binding and the Fab region is available for binding antigen. |
|
Purification |
Use at an assay dependent concentration.
Purification of monoclonal and polyclonal antibodies from culture media, serum, ascites fluid or hybridoma supernatants. |
说明 |
---|
IP
Use at an assay dependent concentration. Isolation of antibody/antigen complexes in immunoprecipitation experiments, since only the Fc region is involved in antibody binding and the Fab region is available for binding antigen. |
Purification
Use at an assay dependent concentration. Purification of monoclonal and polyclonal antibodies from culture media, serum, ascites fluid or hybridoma supernatants. |
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
Datasheet download
文献 (7)
ab193255 被引用在 7 文献中.
- Yang R et al. NHR-80 senses the mitochondrial UPR to rewire citrate metabolism for lipid accumulation in Caenorhabditis elegans. Cell Rep 38:110206 (2022). PubMed: 35021096
- Ma Y miR-4735-3p inhibits cell migration and invasion of gastric cancer by downregulating NEDD9. Oncol Lett 24:253 (2022). PubMed: 35765282
- Yang C & Liu H Both a hypoxia-inducible EYA3 and a histone acetyltransferase p300 function as coactivators of SIX5 to mediate tumorigenesis and cancer progression. Ann Transl Med 10:752 (2022). PubMed: 35957720
- Okpara MO et al. A mass spectrometry-based approach for the identification of Kpnβ1 binding partners in cancer cells. Sci Rep 12:20171 (2022). PubMed: 36418423
- Zhang L et al. S100A11 Promotes Liver Steatosis via FOXO1-Mediated Autophagy and Lipogenesis. Cell Mol Gastroenterol Hepatol 11:697-724 (2021). PubMed: 33075563
- Wang Y et al. CDE-1 suppresses the production of risiRNA by coupling polyuridylation and degradation of rRNA. BMC Biol 18:115 (2020). PubMed: 32887607
- Wu J et al. PHA-4/FoxA senses nucleolar stress to regulate lipid accumulation in Caenorhabditis elegans. Nat Commun 9:1195 (2018). PubMed: 29567958