重组Anti-Proteasome subunit beta type 2/PSMB2抗体[EPR8826(2)(B)] (ab166628)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8826(2)(B)] to Proteasome subunit beta type 2/PSMB2
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Proteasome subunit beta type 2/PSMB2抗体[EPR8826(2)(B)]
参阅全部 Proteasome subunit beta type 2/PSMB2 一抗 -
描述
兔单克隆抗体[EPR8826(2)(B)] to Proteasome subunit beta type 2/PSMB2 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide within Human Proteasome subunit beta type 2/PSMB2. The exact sequence is proprietary.
Database link: P49721 -
阳性对照
- Jurkat, HepG2, HeLa and LoVo cell lysates; Human gastric carcinoma and Human liver tissues; Permeabilized Jurkat cells; Immunoprecipitation pellet from HeLa cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR8826(2)(B) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab166628于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB | (2) |
1/1000 - 1/10000. Predicted molecular weight: 23 kDa.
|
IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
说明 |
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Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 23 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit has a trypsin-like activity. -
序列相似性
Belongs to the peptidase T1B family. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 5690 Human
- Entrez Gene: 26445 Mouse
- Entrez Gene: 29675 Rat
- Omim: 602175 Human
- SwissProt: P49721 Human
- SwissProt: Q9R1P3 Mouse
- SwissProt: P40307 Rat
- Unigene: 471441 Human
see all -
别名
- HC7 I antibody
- Macropain subunit C7 I antibody
- Macropain subunit C7-I antibody
see all
图片
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All lanes : Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8826(2)(B)] (ab166628) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : LoVo cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 23 kDa -
Immunohistochemical analysis of paraffin embedded Human liver tissue labeling Proteasome subunit beta type 2/PSMB2 with ab166628 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human gastric carcinoma tissue labeling Proteasome subunit beta type 2/PSMB2 with ab166628 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Intracellular flow cytometric analysis of permeabilized Jurkat cells using ab166628 at a 1/100 dilution (red) or a Rabbit IgG (negative) (green).
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Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8826(2)(B)] (ab166628) at 1/1000 dilution + Immunoprecipitation pellet from HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 10 µg
Secondary
HRP-conjugated anti-Rabbit IgG preferentially detecting the non-reduced form of Rabbit IgG
Predicted band size: 23 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab166628 被引用在 2 文献中.
- Lee JH et al. Poly-ADP-ribosylation drives loss of protein homeostasis in ATM and Mre11 deficiency. Mol Cell 81:1515-1533.e5 (2021). PubMed: 33571423
- Cholewa BD et al. Large-scale label-free comparative proteomics analysis of polo-like kinase 1 inhibition via the small-molecule inhibitor BI 6727 (Volasertib) in BRAF(V600E) mutant melanoma cells. J Proteome Res 13:5041-50 (2014). WB . PubMed: 24884503