Anti-Proteasome subunit alpha type 6抗体(ab97563)
Key features and details
- Rabbit polyclonal to Proteasome subunit alpha type 6
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-Proteasome subunit alpha type 6抗体
参阅全部 Proteasome subunit alpha type 6 一抗 -
描述
兔多克隆抗体to Proteasome subunit alpha type 6 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Pig, Zebrafish -
免疫原
Recombinant fragment corresponding to Human Proteasome subunit alpha type 6 aa 57-244.
Database link: P60900 -
阳性对照
- WB: 293T, A431, H1299, HeLa, HepG2 and MOLT4 cell lysates IF: A431 cells IHC-P: H1299 xenograft
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97563于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 27 kDa.
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IHC-P |
1/100 - 1/500.
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ICC/IF |
1/100 - 1/200.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 27 kDa. |
IHC-P
1/100 - 1/500. |
ICC/IF
1/100 - 1/200. |
靶标
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功能
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. -
序列相似性
Belongs to the peptidase T1A family. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 5687 Human
- Entrez Gene: 26443 Mouse
- Entrez Gene: 29673 Rat
- Entrez Gene: 171585 Zebrafish
- Omim: 602855 Human
- SwissProt: P60900 Human
- SwissProt: Q9QUM9 Mouse
- SwissProt: P60901 Rat
see all -
别名
- 27 kDa prosomal protein antibody
- IOTA antibody
- Macropain iota chain antibody
see all
图片
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Anti-Proteasome subunit alpha type 6 antibody (ab97563) at 1/1000 dilution + mouse liver lysate at 50 µg
Predicted band size: 27 kDa -
Immunohistochemical analysis of paraffin-embedded mouse lung tissue staining Proteasome subunit alpha type 6 with ab97563 at 1/500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
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ab97563, at a 1/200 dilution, staining Proteasome subunit alpha type 6 in paraformaldehyde-fixed A431 cells by Immunofluorescence analysis. Right image is merged with a DNA probe.
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All lanes : Anti-Proteasome subunit alpha type 6 antibody (ab97563) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : H1299 whole cell lysate
Lane 4 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 27 kDa
12% SDS PAGE -
Anti-Proteasome subunit alpha type 6 antibody (ab97563) at 1/1000 dilution + rat liver lysate at 50 µg
Predicted band size: 27 kDa -
Immunohistochemical analysis of paraffin-embedded mouse urinary bladder tissue staining Proteasome subunit alpha type 6 with ab97563 at 1/500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
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Immunohistochemical analysis of paraffin-embedded mouse liver tissue staining Proteasome subunit alpha type 6 with ab97563 at 1/500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
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ab97563, at a 1/100 dilution, staining Proteasome subunit alpha type 6 in paraffin-embedded H1299 xenograft by Immunohistochemical analysis.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab97563 被引用在 1 文献中.
- Piccinini AM et al. Distinct microenvironmental cues stimulate divergent TLR4-mediated signaling pathways in macrophages. Sci Signal 9:ra86 (2016). WB . PubMed: 27577261