Anti-Proteasome活化剂Subunit 4/PSME4抗体(ab5620)

Submit a review Submit a question References (3)

Key features and details

  • Rabbit polyclonal to Proteasome Activator Subunit 4/PSME4
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Cow, Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-Proteasome活化剂Subunit 4/PSME4抗体
    参阅全部 Proteasome Activator Subunit 4/PSME4 一抗

  • 描述

    兔多克隆抗体to Proteasome活化剂Subunit 4/PSME4

  • 宿主

    Rabbit

  • 经测试应用

    适用于: ICC/IF, WBmore details

  • 种属反应性

    与反应: Mouse, Cow, Human
    预测可用于: Zebrafish

  • 免疫原

    Synthetic peptide corresponding to Human Proteasome Activator Subunit 4/PSME4 aa 1620-1634.
    Sequence:

    TKLPKKRKRDPGSVG

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 常规说明

     This product was previously labelled as Proteasome Activator Subunit 4

     

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    Preservative: 0.05% Sodium azide
  • Concentration information loading...

  • 纯度

    Whole antiserum

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

应用

Our Abpromise guarantee covers the use of ab5620 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/600.
WB 1/1000 - 1/5000. Detects a band of approximately 200 kDa.

靶标

图片

  • Western blot - Anti-Proteasome Activator Subunit 4/PSME4 antibody (ab5620)
    Western blot - Anti-Proteasome Activator Subunit 4/PSME4 antibody (ab5620)
    Anti-Proteasome Activator Subunit 4/PSME4 antibody (ab5620) at 1/2000 dilution + Mouse testis tissue lysate at 25 µg

    Secondary
    HRP-conjugated anti-rabbit

    Developed using the ECL technique.

    Observed band size: 160,200 kDa
    why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-Proteasome Activator Subunit 4/PSME4 antibody (ab5620)
    Immunocytochemistry/ Immunofluorescence - Anti-Proteasome Activator Subunit 4/PSME4 antibody (ab5620)

    ICC/IF image of ab5620 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5620, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

文献 (3)

发表研究结果有使用 ab5620？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab5620 被引用在 3 文献中.

  • Pickering AM & Davies KJ Differential roles of proteasome and immunoproteasome regulators Pa28aß, Pa28? and Pa200 in the degradation of oxidized proteins. Arch Biochem Biophys 523:181-90 (2012). WB ; Mouse . PubMed: 22564544
  • Greenhaff PL  et al. Disassociation between the effects of amino acids and insulin on signaling, ubiquitin ligases, and protein turnover in human muscle. Am J Physiol Endocrinol Metab 295:E595-604 (2008). WB ; Human . PubMed: 18577697
  • Zhang L  et al. Effects of aging and dietary restriction on ubiquitination, sumoylation, and the proteasome in the spleen. FEBS Lett 581:5543-7 (2007). PubMed: 17991438

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

There are currently no Customer reviews or Questions for ab5620.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com