Anti-Proteasome活化剂Subunit 4/PSME4抗体(ab5620)

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Western blot - Anti-Proteasome Activator Subunit 4/PSME4 antibody (ab5620)
  • Western blot - Anti-Proteasome Activator Subunit 4/PSME4 antibody (ab5620)
  • Immunocytochemistry/ Immunofluorescence - Anti-Proteasome Activator Subunit 4/PSME4 antibody (ab5620)

Key features and details

  • Rabbit polyclonal to Proteasome Activator Subunit 4/PSME4
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-Proteasome活化剂Subunit 4/PSME4抗体
    参阅全部 Proteasome Activator Subunit 4/PSME4 一抗

  • 描述

    兔多克隆抗体to Proteasome活化剂Subunit 4/PSME4

  • 宿主

    Rabbit

  • 经测试应用

    适用于: ICC/IF, WBmore details

  • 种属反应性

    与反应: Mouse, Human
    预测可用于: Zebrafish

  • 免疫原

    Synthetic peptide corresponding to Human Proteasome Activator Subunit 4/PSME4 aa 1620-1634.
    Sequence:

    TKLPKKRKRDPGSVG

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 阳性对照

    • WB: Hela, Jurkat whole cell lysate, Mouse testis tissue lysate. ICC: HeLa cells.

  • 常规说明

     This product was previously labelled as Proteasome Activator Subunit 4

     

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    Preservative: 0.05% Sodium azide
  • Concentration information loading...

  • 纯度

    Whole antiserum

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab5620于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
1/600.
WB
1/250 - 1/5000. Detects a band of approximately 200 kDa.
说明
ICC/IF
1/600.
WB
1/250 - 1/5000. Detects a band of approximately 200 kDa.

靶标

  • 功能

    Activates proteasomal cleavage of peptides in an energy-independent manner. May be involved in spermatogenesis. May be involved in DNA repair.

  • 序列相似性

    Contains 6 HEAT repeats.

  • 细胞定位

    Nucleus. Nucleus speckle. Found in nuclear foci following treatment with ionizing radiation, but not with ultraviolet irradiation or H(2).
  • Information by UniProt

  • 数据库链接

    • 别名

      • KIAA0077 antibody
      • PA200 antibody
      • Proteasome Activator 200 kDa antibody
      • Proteasome activator complex subunit 4 antibody
      • Proteasome activator PA200 antibody
      • PSME 4 antibody
      • psme4 antibody
      • PSME4_HUMAN antibody
      see all

    图片

    • Western blot - Anti-Proteasome Activator Subunit 4/PSME4 antibody (ab5620)
      Western blot - Anti-Proteasome Activator Subunit 4/PSME4 antibody (ab5620)
      All lanes : Anti-Proteasome Activator Subunit 4/PSME4 antibody (ab5620) at 1/250 dilution

      Lane 1 : HeLa whole cell extract
      Lane 2 : Jurkat whole cell extract
      Lane 3 : Mouse testis tissue extract
      Lane 4 : Mouse pancreas tissue extract
      Lane 5 : Rat pancreas tissue extract

      Lysates/proteins at 30 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit IgG (H+L) HRP conjugate at 1/4000 dilution


      A 200 kDa band corresponding to PSME4 was observed in HeLa, Jurkat, and mouse testis. In addition, a 140 kDa band corresponding to an isoform of PSME4 was observed across the cell lines and tissues tested. 

      Blocking - 5% skimmed milk

    • Western blot - Anti-Proteasome Activator Subunit 4/PSME4 antibody (ab5620)
      Western blot - Anti-Proteasome Activator Subunit 4/PSME4 antibody (ab5620)
      Anti-Proteasome Activator Subunit 4/PSME4 antibody (ab5620) at 1/2000 dilution + Mouse testis tissue lysate at 25 µg

      Secondary
      HRP-conjugated anti-rabbit

      Developed using the ECL technique.

      Observed band size: 160,200 kDa why is the actual band size different from the predicted?

    • Immunocytochemistry/ Immunofluorescence - Anti-Proteasome Activator Subunit 4/PSME4 antibody (ab5620)
      Immunocytochemistry/ Immunofluorescence - Anti-Proteasome Activator Subunit 4/PSME4 antibody (ab5620)

      ICC/IF image of ab5620 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5620, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    文献 (3)

    发表研究结果有使用 ab5620？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab5620 被引用在 3 文献中.

    • Pickering AM & Davies KJ Differential roles of proteasome and immunoproteasome regulators Pa28aß, Pa28? and Pa200 in the degradation of oxidized proteins. Arch Biochem Biophys 523:181-90 (2012). WB ; Mouse . PubMed: 22564544
    • Greenhaff PL  et al. Disassociation between the effects of amino acids and insulin on signaling, ubiquitin ligases, and protein turnover in human muscle. Am J Physiol Endocrinol Metab 295:E595-604 (2008). WB ; Human . PubMed: 18577697
    • Zhang L  et al. Effects of aging and dietary restriction on ubiquitination, sumoylation, and the proteasome in the spleen. FEBS Lett 581:5543-7 (2007). PubMed: 17991438

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