重组Anti-Proteasome 20S LMP2抗体[EPR13784] (ab187645)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13784] to Proteasome 20S LMP2
- Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Proteasome 20S LMP2抗体[EPR13784]
参阅全部 Proteasome 20S LMP2 一抗 -
描述
兔单克隆抗体[EPR13784] to Proteasome 20S LMP2 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, ICC/IF, IPmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Ramos, Raji, HL60 and A431 cell lysates; A431 and Raji cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR13784 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab187645于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/170.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/1000 - 1/10000. Detects a band of approximately 21 kDa (predicted molecular weight: 23 kDa).
|
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ICC/IF |
1/100.
|
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IP |
1/30 - 1/50.
|
说明 |
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Flow Cyt (Intra)
1/170. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Detects a band of approximately 21 kDa (predicted molecular weight: 23 kDa). |
ICC/IF
1/100. |
IP
1/30 - 1/50. |
靶标
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功能
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB6 by PSMB9 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues. -
序列相似性
Belongs to the peptidase T1B family. -
发展阶段
Highly expressed in immature dendritic cells (at protein level). -
翻译后修饰
Autocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 5698 Human
- Omim: 177045 Human
- SwissProt: P28065 Human
- Unigene: 654585 Human
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别名
- Beta1i antibody
- Large multifunctional peptidase 2 antibody
- Large multifunctional protease 2 antibody
see all
图片
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Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Proteasome 20S LMP2 knockout HAP1 cell lysate (20 µg)
Lanes 1 - 2: Merged signal (red and green). Green - ab187645 observed at 22 and 24 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab187645 was shown to recognize Proteasome 20S LMP2 when Proteasome 20S LMP2 knockout samples were used, along with additional cross-reactive bands. Wild-type and Proteasome 20S LMP2 knockout samples were subjected to SDS-PAGE. ab187645 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging. -
All lanes : Anti-Proteasome 20S LMP2 antibody [EPR13784] (ab187645) at 1/10000 dilution
Lane 1 : Ramos cell lysate
Lane 2 : Raji cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 23 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted? -
All lanes : Anti-Proteasome 20S LMP2 antibody [EPR13784] (ab187645) at 1/2000 dilution
Lane 1 : HL-60 cell lysate
Lane 2 : A431 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 23 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted? -
Western blot analysis of Proteasome 20S LMP2 in Raji cell lysate immunoprecipitated using ab187645 at 1/50 (Lane 1). Lane 2: PBS instead of Raji lysate.
Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
Based on the sequence analysis by BLAST, ab187645 recognizes two isoforms with the predicted MWs of 22kDa and 23kDa respectively.
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Intracellular flow cytometric analysis of 2% paraformaldehyde-fixedRaji cells labeling Proteasome 20S LMP2 with ab187645 at 1/170 dilution compared to a Rabbit monoclonal IgG isotype control, followed byGoat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed A431 cells labeling Proteasome 20S LMP2 with ab187645 at 1/100 dilution. Counter stained with Dapi (blue).
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (1)
ab187645 被引用在 1 文献中.
- Yuan J et al. Transcriptome-wide association study identifies PSMB9 as a susceptibility gene for coal workers' pneumoconiosis. Environ Toxicol 37:2103-2114 (2022). PubMed: 35506645