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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Proteasome

Anti-Proteasome 20S LMP2抗体(ab3328)

  • Datasheet
  • SDS
Reviews (5)Q&A (1)References (27)

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Western blot - Anti-Proteasome 20S LMP2 antibody (ab3328)
  • Western blot - Anti-Proteasome 20S LMP2 antibody (ab3328)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 20S LMP2 antibody (ab3328)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 20S LMP2 antibody (ab3328)

Key features and details

  • Rabbit polyclonal to Proteasome 20S LMP2
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-Proteasome 20S LMP2抗体
    参阅全部 Proteasome 20S LMP2 一抗
  • 描述

    兔多克隆抗体to Proteasome 20S LMP2
  • 宿主

    Rabbit
  • 特异性

    Detects proteasome 20S LMP2.
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Mouse
    Human
    See all applications and species data
  • 免疫原

    Synthetic peptide corresponding to Human Proteasome 20S LMP2 aa 206-219.
    Sequence:

    RVILGNELPKFYDE


    Database link: P28065
    (Peptide available as ab4944)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Proteasome

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Proteasome 20S LMP2 protein (ab105592)

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab3328 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用 Species
IHC-P
Human
WB
Mouse
Human
All applications
Rat
Cow
应用 Ab评论 说明
WB (5)
Use a concentration of 1 µg/ml. (worked at 1/2000 (PMID 17114438))
IHC-P
Use a concentration of 1 µg/ml.
说明
WB
Use a concentration of 1 µg/ml. (worked at 1/2000 (PMID 17114438))
IHC-P
Use a concentration of 1 µg/ml.

靶标

  • 功能

    The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB6 by PSMB9 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues.
  • 序列相似性

    Belongs to the peptidase T1B family.
  • 发展阶段

    Highly expressed in immature dendritic cells (at protein level).
  • 翻译后修饰

    Autocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity.
  • 细胞定位

    Cytoplasm. Nucleus.
  • Target information above from: UniProt accession P28065 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 510593 Cow
    • Entrez Gene: 5698 Human
    • Entrez Gene: 16912 Mouse
    • Entrez Gene: 24967 Rat
    • Omim: 177045 Human
    • SwissProt: Q3SZC2 Cow
    • SwissProt: P28065 Human
    • SwissProt: P28076 Mouse
    • SwissProt: P28077 Rat
    • Unigene: 654585 Human
    • Unigene: 390983 Mouse
    • Unigene: 13686 Rat
    see all
  • 别名

    • Beta1i antibody
    • Large multifunctional peptidase 2 antibody
    • Large multifunctional protease 2 antibody
    • LMP 2 antibody
    • LMP2 antibody
    • Low molecular mass protein 2 antibody
    • Macropain chain 7 antibody
    • MGC70470 antibody
    • Multicatalytic endopeptidase complex chain 7 antibody
    • OTTHUMP00000062982 antibody
    • Proteasome (prosome macropain) subunit beta type 9 antibody
    • proteasome (prosome, macropain) subunit, beta type, 9 (large multifunctional peptidase 2) antibody
    • Proteasome beta 9 subunit antibody
    • Proteasome catalytic subunit 1i antibody
    • Proteasome chain 7 antibody
    • Proteasome related gene 2 antibody
    • Proteasome subunit beta 6i antibody
    • Proteasome subunit beta type 9 antibody
    • Proteasome subunit beta type-9 antibody
    • Proteasome subunit beta-1i antibody
    • PSB9_HUMAN antibody
    • PSMB 9 antibody
    • PSMB6i antibody
    • PSMB9 antibody
    • Really interesting new gene 12 protein antibody
    • RING 12 antibody
    • RING12 antibody
    • RING12 protein antibody
    see all

图片

  • Western blot - Anti-Proteasome 20S LMP2 antibody (ab3328)
    Western blot - Anti-Proteasome 20S LMP2 antibody (ab3328)
    All lanes : Anti-Proteasome 20S LMP2 antibody (ab3328) at 2 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) treated with 100 ng/mL IFN gamma for 72 hours, whole cell lysate
    Lane 3 : THP-1 (human monocytic leukemia cell line) whole cell lysate
    Lane 4 : THP-1 (human monocytic leukemia cell line) treated with 50 ng/mL PMA for 48 hours and 200 ng/mL LPS for 24 hours, whole cell lysate
    Lane 5 : U937 (human histiocytic lymphoma cell line) whole cell lysate
    Lane 6 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
    Lane 7 : Ramos (human Burkitt's lymphoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG (H+L) HRP conjugate at 0.4 µg/ml

    Developed using the ECL technique.
  • Western blot - Anti-Proteasome 20S LMP2 antibody (ab3328)
    Western blot - Anti-Proteasome 20S LMP2 antibody (ab3328)

    Western blot of proteasome 20S LMP2 in 50 ug mouse embryo fibroblast cell extract using ab3328.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 20S LMP2 antibody (ab3328)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 20S LMP2 antibody (ab3328)
    ab3328 (1µg/ml) staining Proteasome 20S LMP2 in human heart using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of nuclear and cytoplasmic cell compartments.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1/ EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 20S LMP2 antibody (ab3328)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 20S LMP2 antibody (ab3328)Image from Nguyen TP et al., Am J Pathol. 2010;176(2):893-902. Fig 8(A).; doi: 10.2353/ajpath.2010.090390 with permission from Elsevier.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue (neurons in HIV encephalitis) sections labelling LMP2 with ab3328 at 1/1000. Immunofluorescence staining for LMP2 shows colocalization with the neuronal marker NeuN. LMP2 is present in granular deposits in the parikaryon. Scale bar = 10 μm.

实验方案

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (27)

发表研究结果有使用 ab3328?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab3328 被引用在 27 文献中.

  • Lee MJ  et al. H727 cells are inherently resistant to the proteasome inhibitor carfilzomib, yet require proteasome activity for cell survival and growth. Sci Rep 9:4089 (2019). PubMed: 30858500
  • Jimenez-Guardeño JM  et al. Immunoproteasome activation enables human TRIM5a restriction of HIV-1. Nat Microbiol 4:933-940 (2019). PubMed: 30886358
  • Babaer D  et al. Methylselenol producing selenocompounds enhance the efficiency of mammaglobin-A peptide vaccination against breast cancer cells. Oncol Lett 18:6891-6898 (2019). PubMed: 31807192
  • Studencka-Turski M  et al. Molecular Insight Into the IRE1a-Mediated Type I Interferon Response Induced by Proteasome Impairment in Myeloid Cells of the Brain. Front Immunol 10:2900 (2019). PubMed: 31921161
  • Chen S  et al. Immunoproteasome dysfunction augments alternative polarization of alveolar macrophages. Cell Death Differ 23:1026-37 (2016). PubMed: 26990663
View all Publications for this product

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1-5 of 5 Abreviews or Q&A

Western blot abreview for Anti-Proteasome 20S LMP2 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (THP-1 cells)
Gel Running Conditions
Reduced Denaturing (4 - 12%)
Loading amount
50000 cells
Specification
THP-1 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Read More

Abcam user community

Verified customer

提交于 May 21 2019

Western blot abreview for Anti-Proteasome 20S LMP2 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (4-15%)
Sample
Human Cell lysate - whole cell (Lymphoma)
Specification
Lymphoma
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Dr. Satyendra Tripathi

Verified customer

提交于 Oct 07 2014

Western blot abreview for Anti-Proteasome 20S LMP2 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (dendritic cell)
Loading amount
1e+006 cells
Specification
dendritic cell
Gel Running Conditions
Non-reduced Denaturing
Blocking step
BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Read More

Abcam user community

Verified customer

提交于 Jan 05 2010

Western blot abreview for Anti-Proteasome 20S LMP2 antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Purified protein (heart and spleen)
Loading amount
1 µg
Specification
heart and spleen
Gel Running Conditions
Reduced Denaturing (12,5%)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More

Abcam user community

Verified customer

提交于 Sep 03 2008

Western blot abreview for Anti-Proteasome 20S LMP2 antibody

Average
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (B-LCL)
Loading amount
80000 cells
Specification
B-LCL
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 18°C
Read More

Abcam user community

Verified customer

提交于 Jun 25 2007

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