Anti-Proteasome 20S LMP2抗体(ab3328)
Key features and details
- Rabbit polyclonal to Proteasome 20S LMP2
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Proteasome 20S LMP2抗体
参阅全部 Proteasome 20S LMP2 一抗 -
描述
兔多克隆抗体to Proteasome 20S LMP2 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Cow
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免疫原
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阳性对照
- WB: HeLa cells, mouse embryo fibroblast cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (5) |
Use a concentration of 1 µg/ml. (worked at 1/2000 (PMID 17114438))
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| 说明 |
|---|
|
WB
Use a concentration of 1 µg/ml. (worked at 1/2000 (PMID 17114438)) |
靶标
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功能
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB6 by PSMB9 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues. -
序列相似性
Belongs to the peptidase T1B family. -
发展阶段
Highly expressed in immature dendritic cells (at protein level). -
翻译后修饰
Autocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 510593 Cow
- Entrez Gene: 5698 Human
- Entrez Gene: 16912 Mouse
- Entrez Gene: 24967 Rat
- Omim: 177045 Human
- SwissProt: Q3SZC2 Cow
- SwissProt: P28065 Human
- SwissProt: P28076 Mouse
see all -
别名
- Beta1i antibody
- Large multifunctional peptidase 2 antibody
- Large multifunctional protease 2 antibody
see all
图片
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Western blot - Anti-Proteasome 20S LMP2 antibody (ab3328)All lanes : Anti-Proteasome 20S LMP2 antibody (ab3328) at 2 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) treated with 100 ng/mL IFN gamma for 72 hours, whole cell lysate
Lane 3 : THP-1 (human monocytic leukemia cell line) whole cell lysate
Lane 4 : THP-1 (human monocytic leukemia cell line) treated with 50 ng/mL PMA for 48 hours and 200 ng/mL LPS for 24 hours, whole cell lysate
Lane 5 : U937 (human histiocytic lymphoma cell line) whole cell lysate
Lane 6 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lane 7 : Ramos (human Burkitt's lymphoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) HRP conjugate at 0.4 µg/ml
Developed using the ECL technique. -
Western blot - Anti-Proteasome 20S LMP2 antibody (ab3328)Western blot of proteasome 20S LMP2 in 50 ug mouse embryo fibroblast cell extract using ab3328.
数据表及文件
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Datasheet download
文献 (28)
ab3328 被引用在 28 文献中.
- Kalaora S et al. Immunoproteasome expression is associated with better prognosis and response to checkpoint therapies in melanoma. Nat Commun 11:896 (2020). PubMed: 32060274
- Lee MJ et al. H727 cells are inherently resistant to the proteasome inhibitor carfilzomib, yet require proteasome activity for cell survival and growth. Sci Rep 9:4089 (2019). PubMed: 30858500
- Jimenez-Guardeño JM et al. Immunoproteasome activation enables human TRIM5a restriction of HIV-1. Nat Microbiol 4:933-940 (2019). PubMed: 30886358
- Babaer D et al. Methylselenol producing selenocompounds enhance the efficiency of mammaglobin-A peptide vaccination against breast cancer cells. Oncol Lett 18:6891-6898 (2019). PubMed: 31807192
- Studencka-Turski M et al. Molecular Insight Into the IRE1a-Mediated Type I Interferon Response Induced by Proteasome Impairment in Myeloid Cells of the Brain. Front Immunol 10:2900 (2019). PubMed: 31921161
