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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Proteasome

Anti-Proteasome 20S C2/HC2抗体(ab3325)

  • Datasheet
  • SDS
Reviews (2)Q&A (1)References (28)

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Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S C2/HC2 antibody (ab3325)
  • Western blot - Anti-Proteasome 20S C2/HC2 antibody (ab3325)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 20S C2/HC2 antibody (ab3325)
  • Western blot - Anti-Proteasome 20S C2/HC2 antibody (ab3325)

Key features and details

  • Rabbit polyclonal to Proteasome 20S C2/HC2
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-Proteasome 20S C2/HC2抗体
    参阅全部 Proteasome 20S C2/HC2 一抗
  • 描述

    兔多克隆抗体to Proteasome 20S C2/HC2
  • 宿主

    Rabbit
  • 特异性

    Detects proteasome 20S C2/HC2 subunit.

  • 经测试应用

    适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性

    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human Proteasome 20S C2/HC2 aa 249-263 (C terminal).
    Sequence:

    PADEPAEKADEPMEH


    Database link: P25786
    (Peptide available as ab4943)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

     This product was previously labelled as Proteasome 20S C2

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Proteasome

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Recombinant Human Proteasome 20S C2/HC2 protein (ab116156)
  • Recombinant Protein

    • Recombinant Human Proteasome 20S C2/HC2 protein (ab116156)

应用

Our Abpromise guarantee covers the use of ab3325 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 3 µg/ml.
IHC-P 1/1000. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
ICC/IF Use a concentration of 2 µg/ml.

靶标

  • 功能

    The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Mediates the lipopolysaccharide-induced signal transduction in the macrophage proteasome (By similarity). Might be involved in the anti-inflammatory response of macrophages during the interaction with C.albicans heat-inactivated cells.
  • 序列相似性

    Belongs to the peptidase T1A family.
  • 细胞定位

    Cytoplasm. Nucleus.
  • Target information above from: UniProt accession P25786 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 5682 Human
    • Omim: 602854 Human
    • SwissProt: P25786 Human
    • Unigene: 102798 Human
    • 别名

      • 30 kDa prosomal protein antibody
      • HC 2 antibody
      • HC2 antibody
      • Macropain subunit C2 antibody
      • Macropain subunit nu antibody
      • MGC14542 antibody
      • MGC14575 antibody
      • MGC14751 antibody
      • MGC1667 antibody
      • MGC21459 antibody
      • MGC22853 antibody
      • MGC23915 antibody
      • Multicatalytic endopeptidase complex subunit C2 antibody
      • NU antibody
      • PROS 30 antibody
      • PROS-30 antibody
      • PROS30 antibody
      • Proteasome (prosome macropain) subunit alpha type 1 antibody
      • Proteasome alpha 1 subunit antibody
      • Proteasome component C2 antibody
      • Proteasome nu chain antibody
      • Proteasome subunit alpha type 1 antibody
      • Proteasome subunit alpha type I antibody
      • Proteasome subunit alpha type-1 antibody
      • Proteasome subunit nu antibody
      • Protein P30 33K antibody
      • PSA1_HUMAN antibody
      • PSC 2 antibody
      • PSC2 antibody
      • PSMA 1 antibody
      • psmA1 antibody
      see all

    图片

    • Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S C2/HC2 antibody (ab3325)
      Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S C2/HC2 antibody (ab3325)

      Immunofluorescence analysis of 70% confluent log phase MDA-MB-231 (Human breast adenocarcinoma cell line) cells labeling Proteasome 20S C2/HC2 (green) with ab3325 at 2 µg/mL. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ab3325 in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) secondary antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin. Panel d represents the merged image showing cytoplasmic localization. Panel e shows the control without primary antibody. The images were captured at 60X magnification.

    • Western blot - Anti-Proteasome 20S C2/HC2 antibody (ab3325)
      Western blot - Anti-Proteasome 20S C2/HC2 antibody (ab3325)
      All lanes : Anti-Proteasome 20S C2/HC2 antibody (ab3325) at 2 µg/ml

      Lane 1 : MDA-MB-231 (Human breast adenocarcinoma cell line) whole cell lysate
      Lane 2 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
      Lane 3 : PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate
      Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
      Lane 5 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit IgG (H+L) HRP cpnjugate at 0.4 µg/ml

      Developed using the ECL technique.


      Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel, XCell SureLock™ Electrophoresis System and Novex® Sharp Pre-Stained Protein Standard. Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 20S C2/HC2 antibody (ab3325)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 20S C2/HC2 antibody (ab3325)

      ab3325 (1µg/ml) staining Proteasome 20S C2/HC2 in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic and nuclear staining.
      Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

    • Western blot - Anti-Proteasome 20S C2/HC2 antibody (ab3325)
      Western blot - Anti-Proteasome 20S C2/HC2 antibody (ab3325)
      Anti-Proteasome 20S C2/HC2 antibody (ab3325) at 3 µg/ml + CHO (Chinese hamster ovary cell line) whole cell lysate

    实验方案

    • Western blot protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet
    • SDS
  • 文献 (28)

    发表研究结果有使用 ab3325?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab3325 被引用在 28 文献中.

    • Christensen IB  et al. Genetic disruption of slc4a10 alters the capacity for cellular metabolism and vectorial ion transport in the choroid plexus epithelium. Fluids Barriers CNS 17:2 (2020). PubMed: 31906971
    • Murali SK  et al. The Deubiquitylase USP4 Interacts with the Water Channel AQP2 to Modulate Its Apical Membrane Accumulation and Cellular Abundance. Cells 8:N/A (2019). PubMed: 30901874
    • Saglar Ozer E  et al. Molecular characterization of an aquaporin-2 mutation causing a severe form of nephrogenic diabetes insipidus. Cell Mol Life Sci N/A:N/A (2019). PubMed: 31302751
    • Sun C  et al. Cellular Requirements for Sensing and Elimination of Incoming HSV-1 DNA and Capsids. J Interferon Cytokine Res 39:191-204 (2019). PubMed: 30855198
    • Auvin S  et al. A molecule inducing androgen receptor degradation and selectively targeting prostate cancer cells. Life Sci Alliance 2:N/A (2019). PubMed: 31431473
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-3 of 3 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Proteasome 20S C2/HC2 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (RPE-1)
    Permeabilization
    Yes - 0.5% Triton X100 in PBS
    Specification
    RPE-1
    Fixative
    Formaldehyde
    Read More

    Dr. Kirk Mcmanus

    Verified customer

    提交于 Oct 26 2007

    Western blot abreview for Anti-Proteasome 20S C2/HC2 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (heart cells)
    Specification
    heart cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Read More

    Ms. ping wang

    Verified customer

    提交于 Jan 27 2006

    Question

    Is Proteasome 20s C2 the same protein as Proteasome subunit alpha type I, with swissprot nº P25786?

    Read More

    Abcam community

    Verified customer

    Asked on Oct 13 2003

    Answer

    Yes, Proteasome 20s C2 is the same as Proteasome subunit alpha type I. I just BLASTed the immunogen sequence and the sequence is pulls out is the SwissProt accession number that you mentioned.

    Read More

    Asdf Edo

    Abcam Scientific Support

    回复于 Oct 13 2003

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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