Anti-Proteasome 20S C2/HC2抗体(ab3325)
Key features and details
- Rabbit polyclonal to Proteasome 20S C2/HC2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human, Chinese hamster
- Isotype: IgG
概述
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产品名称
Anti-Proteasome 20S C2/HC2抗体
参阅全部 Proteasome 20S C2/HC2 一抗 -
描述
兔多克隆抗体to Proteasome 20S C2/HC2 -
宿主
Rabbit -
特异性
Detects proteasome 20S C2/HC2 subunit.
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经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human, Chinese hamster -
免疫原
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常规说明
This product was previously labelled as Proteasome 20S C2
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
应用 | Ab评论 | 说明 |
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WB | (1) |
Use a concentration of 1 - 3 µg/ml.
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IHC-P |
1/1000. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
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ICC/IF | (1) |
Use a concentration of 2 µg/ml.
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说明 |
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WB
Use a concentration of 1 - 3 µg/ml. |
IHC-P
1/1000. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 2 µg/ml. |
靶标
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功能
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Mediates the lipopolysaccharide-induced signal transduction in the macrophage proteasome (By similarity). Might be involved in the anti-inflammatory response of macrophages during the interaction with C.albicans heat-inactivated cells. -
序列相似性
Belongs to the peptidase T1A family. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 5682 Human
- Omim: 602854 Human
- SwissProt: P25786 Human
- Unigene: 102798 Human
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别名
- 30 kDa prosomal protein antibody
- HC 2 antibody
- HC2 antibody
see all
图片
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Immunofluorescence analysis of 70% confluent log phase MDA-MB-231 (Human breast adenocarcinoma cell line) cells labeling Proteasome 20S C2/HC2 (green) with ab3325 at 2 µg/mL. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ab3325 in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) secondary antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin. Panel d represents the merged image showing cytoplasmic localization. Panel e shows the control without primary antibody. The images were captured at 60X magnification.
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All lanes : Anti-Proteasome 20S C2/HC2 antibody (ab3325) at 2 µg/ml
Lane 1 : MDA-MB-231 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 3 : PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate
Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 5 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) HRP cpnjugate at 0.4 µg/ml
Developed using the ECL technique.Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel, XCell SureLock™ Electrophoresis System and Novex® Sharp Pre-Stained Protein Standard. Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 20S C2/HC2 antibody (ab3325)
ab3325 (1µg/ml) staining Proteasome 20S C2/HC2 in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic and nuclear staining.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required. -
Anti-Proteasome 20S C2/HC2 antibody (ab3325) at 3 µg/ml + CHO (Chinese hamster ovary cell line) whole cell lysate
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (28)
ab3325 被引用在 28 文献中.
- Christensen IB et al. Genetic disruption of slc4a10 alters the capacity for cellular metabolism and vectorial ion transport in the choroid plexus epithelium. Fluids Barriers CNS 17:2 (2020). PubMed: 31906971
- Murali SK et al. The Deubiquitylase USP4 Interacts with the Water Channel AQP2 to Modulate Its Apical Membrane Accumulation and Cellular Abundance. Cells 8:N/A (2019). PubMed: 30901874
- Saglar Ozer E et al. Molecular characterization of an aquaporin-2 mutation causing a severe form of nephrogenic diabetes insipidus. Cell Mol Life Sci N/A:N/A (2019). PubMed: 31302751
- Sun C et al. Cellular Requirements for Sensing and Elimination of Incoming HSV-1 DNA and Capsids. J Interferon Cytokine Res 39:191-204 (2019). PubMed: 30855198
- Auvin S et al. A molecule inducing androgen receptor degradation and selectively targeting prostate cancer cells. Life Sci Alliance 2:N/A (2019). PubMed: 31431473