Anti-Proteasome 20S alpha + beta抗体(ab22673)


  • 产品名称
    Anti-Proteasome 20S alpha + beta抗体
  • 描述
    兔多克隆抗体to Proteasome 20S alpha + beta
  • 宿主
  • 经测试应用
    适用于: ICC, IHC-Fr, WB, IP, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Saccharomyces cerevisiae
  • 免疫原

    Tissue/ cell preparation: Proteasomal preparation isolated from human red blood cells.

  • 阳性对照
    • Human and yeast 20S proteasome and tissue/cell preparations including human placental proteasome preparation, a HeLa cell lysate, RSV 3T3 mouse fibroblast cell lysate, and yeast whole cell extract.
  • 常规说明
    Dilute to working concentration in PBS pH 7.2- 7.4, and store at 2 to 4°C (do not freeze). Use diluted antibody within 1 month.



Our Abpromise guarantee covers the use of ab22673 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC Use at an assay dependent concentration. PubMed: 23840376
IHC-Fr Use at an assay dependent concentration.
WB 1/1000 - 1/10000. Predicted molecular weight: 25 - 30 kDa.
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. See Abreview.



  • ab22673 staining human Hela cells by ICC/IF. Cells were formaldehyde fixed, permeabilized in 0.1% Triton and blocked in 3% BSA for 15 minutes at room temperature. The primary antibody was diluted 1/2000 (3% BSA) and incubated with sample for 1 hour. An Alexa Fluor® 488 conjugated goat polyclonal to rabbit, diluted 1/1000 was used as secondary.

    See Abreview

  • All lanes : Anti-Proteasome 20S alpha + beta antibody (ab22673) at 1/2000 dilution

    All lanes : Whole tissue lysate prepared from rat prefrontal cortex

    Lysates/proteins at 20 µg per lane.

    All lanes : Goat anti-rabbit IgG-HRP at 1/5000 dilution

    Developed using the ECL technique.

    Predicted band size: 25 - 30 kDa

    Exposure time: 5 minutes

    Blocked with 5% milk for 30 minutes at 27°C.

    See Abreview

  • Immunofluorescence analysis of HeLa cells, staining Proteasome 20S alpha + beta with ab22673.

    Cells were fixed with formaldehyde and permeabilized using 0.1% Triton X-100. Samples were incubated with primary antibody (1/1000) fr 1 hour at room temperature. An AlexaFluor®555-conjugated donkey anti-rabbit IgG was used as the secondary antibody.


This product has been referenced in:
  • Steen KA  et al. FABP4/aP2 Regulates Macrophage Redox Signaling and Inflammasome Activation via Control of UCP2. Mol Cell Biol 37:N/A (2017). WB ; Mouse . Read more (PubMed: 27795298) »
  • Zhao Y  et al. Essential role of proteasomes in maintaining self-renewal in neural progenitor cells. Sci Rep 6:19752 (2016). WB, ICC/IF ; Mouse . Read more (PubMed: 26804982) »

See all 13 Publications for this product


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I am sorry but as you are aware we do not currently have any of the Anti-Proteasome 20S alpha + beta antibody (ab22673). As discussed over the phone, I have had a look for antibodies which may serve as su...

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I can change the antibody on your current order from ab22673 for ab109525 if you would like. As this is in stock, if you let me know you would like for me to do this, it can be with you by tomorrow.

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Unfortunately, we do not have a direct alternative for the Anti-Proteasome 20S alpha + beta antibody (ab22673). This antibody detects a combination of alpha and beta proteasome su...

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The credit note ID is ***.

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I would be happyto issue a credit note for the customer. Do you have the order number or PO number that this was ordered on? I found one going to Interlab from February 7, o...

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I do have a few additional questions, so that I better understand the situation.

The secondary is listed as HRP conjugated, and the detection system is list...

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DISCOUNT CODE: *** Expiration date: February 20th, 2012 I am very pleased to hear you would like to accept our offer and test ab22673 in rat. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submi...

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Thank you very much for your interest in ab22673. To our knowledge, ab22673 has not been tested in rat. Therefore, I can offer a discount off a future purchase if you buy ab22673 now, test it in rat and submit feedback to us in the form of an ...

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