重组Anti-Proteasome 19S S5A/ASF抗体[EPR8936] (ab137109)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8936] to Proteasome 19S S5A/ASF
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Proteasome 19S S5A/ASF抗体[EPR8936]
参阅全部 Proteasome 19S S5A/ASF 一抗 -
描述
兔单克隆抗体[EPR8936] to Proteasome 19S S5A/ASF -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WBmore details
不适用于: ICC/IF or IHC-P -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human Proteasome 19S S5A/ASF aa 300-400. The exact sequence is proprietary.
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阳性对照
- Jurkat, K562, HepG2, C6, Raw264.7, PC-12 and NIH/3T3 cell lysates; K562 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20ºC. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR8936 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab137109于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
|
说明 |
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Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 40 kDa. |
靶标
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功能
Binds and presumably selects ubiquitin-conjugates for destruction. Displays selectivity for longer polyubiquitin chains. Modulates intestinal fluid secretion. -
序列相似性
Belongs to the proteasome subunit S5A family.
Contains 2 UIM (ubiquitin-interacting motif) repeats.
Contains 1 VWFA domain. -
结构域
The 2 UIM motifs are involved in the binding to a multi-ubiquitin chain in a cooperative way. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. - Information by UniProt
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数据库链接
- Entrez Gene: 5710 Human
- Entrez Gene: 19185 Mouse
- Entrez Gene: 83499 Rat
- Omim: 601648 Human
- SwissProt: P55036 Human
- SwissProt: O35226 Mouse
- Unigene: 505059 Human
- Unigene: 2261 Mouse
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别名
- 26S protease subunit S5a antibody
- 26S proteasome non ATPase regulatory subunit 4 antibody
- 26S proteasome non-ATPase regulatory subunit 4 antibody
see all
图片
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All lanes : Anti-Proteasome 19S S5A/ASF antibody [EPR8936] (ab137109) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : K562 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : C6 cell lysate
Lane 5 : Raw264.7 cell lysate
Lane 6 : PC-12 cell lysate
Lane 7 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-labelled Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 40 kDa -
Intracellular flow cytometric analysis of permeabilized K562 cells labelling Proteasome 19S S5A/ASF with ab137109 at 1/100 dilution (red). Negative Rabbit IgG control shown in green.
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab137109 被引用在 6 文献中.
- Chen L et al. Coordinated regulation of the ribosome and proteasome by PRMT1 in the maintenance of neural stemness in cancer cells and neural stem cells. J Biol Chem 297:101275 (2021). PubMed: 34619150
- de la Fuente AG et al. Changes in the Oligodendrocyte Progenitor Cell Proteome with Ageing. Mol Cell Proteomics 19:1281-1302 (2020). PubMed: 32434922
- Cai MJ et al. Inhibition of PSMD4 blocks the tumorigenesis of hepatocellular carcinoma. Gene 702:66-74 (2019). PubMed: 30930224
- Liu Y et al. CCT3 acts upstream of YAP and TFCP2 as a potential target and tumour biomarker in liver cancer. Cell Death Dis 10:644 (2019). PubMed: 31501420
- Scott MR et al. Protein Expression of Proteasome Subunits in Elderly Patients with Schizophrenia. Neuropsychopharmacology 41:896-905 (2016). PubMed: 26202105
- Wu HY et al. Structural basis of antizyme-mediated regulation of polyamine homeostasis. Proc Natl Acad Sci U S A 112:11229-34 (2015). PubMed: 26305948