蛋白酶抑制剂(EDTA-free) (ab201111)
概述
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产品名称
蛋白酶抑制剂(EDTA-free) -
产品概述
Abcam’s protease inhibitor cocktails inhibit a variety of endogenous proteases to protect your protein samples from degradation.
Ideal for mammalian cell/tissue lysates and bacterial cell extracts using metal chelation chromatography.
Target proteases - serine proteases, aminopeptidase B, leucine aminopeptidase, cysteine proteases, aspartic proteases
Inhibitors included - AEBSF hydrochloride, aprotinin (bovine lung), bestatin, E-64, leupeptin hemisulfate, pepstatin A
Form: Supplied in DMSO (1 ml)
Preparation: The recommended starting dilution is 1:200
Visit our inhibitor cocktails page for more information on our complete range of convenient and easy to use protease and phosphatase inhibitor cocktails.
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说明
This product is manufactured by BioVision, an Abcam company and was previously called K272 EZBlock™ Protease Inhibitor Cocktail, EDTA-Free. K272-1 is the same size as the 1 ml size of ab201111.
Individual components and concentrations:
AEBSF hydrochloride: serine protease inhibitor (100 mM)
Aprotinin (bovine lung): serine protease inhibitor (0.08 mM)
Bestatin: aminopeptidase B and leucine aminopeptidase inhibitor (5 mM)
E-64: cysteine protease inhibitor (1.5 mM)
Leupeptin hemisulfate: serine/cysteine protease inhibitor (2 mM)
Pepstatin A: aspartic protease inhibitor (1 mM)
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 ml Protease Inhibitor Cocktail 1 x 1ml
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab201111 被引用在 6 文献中.
- Wen JJ et al. The Genetic Evidence of Burn-Induced Cardiac Mitochondrial Metabolism Dysfunction. Biomedicines 8:N/A (2020). PubMed: 33287280
- Hou T et al. Sevoflurane preconditioning attenuates hypoxia/reoxygenation injury of H9c2 cardiomyocytes by activation of the HIF-1/PDK-1 pathway. PeerJ 8:e10603 (2020). PubMed: 33391885
- Xiao G et al. Transferrin 1 Functions in Iron Trafficking and Genetically Interacts with Ferritin in Drosophila melanogaster. Cell Rep 26:748-758.e5 (2019). PubMed: 30650364
- Michalek K & Grabowska M Investigating cellular location of aquaporins in the bovine kidney. A new view on renal physiology in cattle. Res Vet Sci 125:162-169 (2019). PubMed: 31233960
- Song M et al. Trimetazidine restores the positive adaptation to exercise training by mitigating statin-induced skeletal muscle injury. J Cachexia Sarcopenia Muscle 9:106-118 (2018). IF . PubMed: 29152896
- Wu X et al. Baicalin inhibits PDGF-BB-induced hepatic stellate cell proliferation, apoptosis, invasion, migration and activation via the miR-3595/ACSL4 axis. Int J Mol Med 41:1992-2002 (2018). PubMed: 29393361