Key features and details
- Mouse monoclonal [T6] to Prolactin Receptor/PRL-R
- Suitable for: IHC-P, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG2a
参阅全部 Prolactin Receptor/PRL-R 一抗
描述小鼠单克隆抗体[T6] to Prolactin Receptor/PRL-R
Detects Prolactin Receptor/PRL-R in rat tissues. This antibody does not cross-react with Growth Hormone (GH) receptor. By Western blot, this antibody detects an ~42 kDa protein representing Prolactin Receptor/PRL-R in NB2 cell lysate. Immunohistochemical staining of Prolactin Receptor/PRL-R in NB2 cells yields a staining pattern consistent with cytoplasmic vesicular staining. This antibody has also been used to inhibit the binding of prolactin to Prolactin Receptor/PRL-R in vitro.
经测试应用适用于: IHC-P, ICC/IFmore details
种属反应性与反应: Rat, Human
Other Immunogen Type corresponding to Rat Prolactin Receptor/PRL-R. Purified rat liver PRL receptor.
- IHC-P: Rat pituitary gland tissue. ICC: C6, H-4-II-E, SW480 cells.
Previously labelled as Prolactin Receptor.
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Concentration information loading...
纯度Protein A purified
Primary antibody说明Prolactin (PRL) is a hormone involved in a variety of important functions including ion transport and osmoregulation, stimulation of milk, protein synthesis as well as the regulation of numerous reproductive functions. PRL exerts its influence on different cell types through a signal transduction pathway which begins with the binding of the hormone to a transmembrane PRL receptor. Immunoreactive PRL receptor, a member of the cytokine receptor family, varies in size (short and long forms) with tissue source and species, from ~40 kDa to 100 kDa. The PRL receptor consists of at least three separate domains: an extracellular region with 5 cysteines which contains the prolactin binding site, a single transmembrane domain and a cytoplasmic region, the length of which appears to influence ligand binding and regulate cellular function.
Our Abpromise guarantee covers the use of ab2773 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/25 - 1/200.|
功能This is a receptor for the anterior pituitary hormone prolactin (PRL). Isoform 4 is unable to transduce prolactin signaling. Isoform 6 is unable to transduce prolactin signaling.
组织特异性Expressed in breast, placenta, kidney, liver and pancreas.
序列相似性Belongs to the type I cytokine receptor family. Type 1 subfamily.
Contains 2 fibronectin type-III domains.
结构域The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation.
细胞定位Secreted and Membrane.
- Information by UniProt
- AI987712 antibody
- CLONE SPM213 antibody
- CPRLP antibody
Immunocytochemical analysis of C6 (Rat glial tumor cell line) cells labeling Prolactin Receptor/PRL-R. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incbated without (control) or with ab2773 (1:200) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Prolactin Receptor/PRL-R staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
Immunocytochemical analysis of H-4-II-E (Rat hepatoma cell line) cells labeling Prolactin Receptor/PRL-R. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incbated without (control) or with ab2773 (1:200) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Prolactin Receptor/PRL-R staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
Immunocytochemical analysis of in SW480 (Human colorectal adenocarcinoma cell line) cells labeling Prolactin Receptor/PRL-R. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incbated without (control) or with ab2773 (1:200) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Prolactin Receptor/PRL-R staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
Immunohistochemical analysis of normal biopsies of deparaffinized rat pituitary gland tissue labeling Prolactin Receptor/PRL-R. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and incubated with ab2773 (1:50) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
ab2773 被引用在 6 文献中.
- Dardenne M et al. Prolactin receptor expression in human hematopoietic tissues analyzed by flow cytofluorometry. Endocrinology 134:2108-14 (1994). PubMed: 8156910
- Rui H et al. JAK2 activation and cell proliferation induced by antibody-mediated prolactin receptor dimerization. Endocrinology 135:1299-306 (1994). PubMed: 7925093
- Gagnerault MC et al. Expression of prolactin receptors in murine lymphoid cells in normal and autoimmune situations. J Immunol 150:5673-81 (1993). PubMed: 8515082
- Dardenne M et al. Identification and functional activity of prolactin receptors in thymic epithelial cells. Proc Natl Acad Sci U S A 88:9700-4 (1991). PubMed: 1946390
- Rozakis-Adcock M & Kelly PA Mutational analysis of the ligand-binding domain of the prolactin receptor. J Biol Chem 266:16472-7 (1991). PubMed: 1885580
- Okamura H et al. Characterization and applications of monoclonal antibodies to the prolactin receptor. Endocrinology 124:2499-508 (1989). PubMed: 2707162