重组Anti-Progesterone抗体[EPPTX-R1] (ab215527)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPPTX-R1] to Progesterone
- Suitable for: Competitive ELISA
- Reacts with: Species independent
Related conjugates and formulations
概述
-
产品名称
Anti-Progesterone抗体[EPPTX-R1]
参阅全部 Progesterone 一抗 -
描述
兔单克隆抗体[EPPTX-R1] to Progesterone -
宿主
Rabbit -
经测试应用
适用于: Competitive ELISAmore details -
种属反应性
与反应: Species independent -
免疫原
Chemical/ Small Molecule corresponding to Human Progesterone. The immunogen is 11 alpha-Hydroxyprogesterone hemisuccinyl-CH3 BSA.
-
阳性对照
- Competitive ELISA: Progesterone
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPPTX-R1 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab215527于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Competitive ELISA |
Use at an assay dependent concentration.
Use 3-5 ng/ml. |
说明 |
---|
Competitive ELISA
Use at an assay dependent concentration. Use 3-5 ng/ml. |
靶标
-
相关性
Progesterone plays a central role in the reproductive events associated with the establishment and maintenance of pregnancy. Progesterone receptor, a member of the steroid receptor superfamily, mediates the physiologic effects of progesterone. The PGR gene uses separate promoters and translational start sites to produce 2 isoforms, PRA and PRB, which are identical except for an additional 165 amino acids present only in the N terminus of PRB. Although PRA and PRB share several structural domains, they are distinct transcription factors that mediate their own response genes and physiologic effects with little overlap. It is composed of three domains: a modulating N terminal domain, a DNA binding domain and a C terminal steroid binding domain. Progesterone levels 1. men 30-60 pg/0.1ml 2. women pre ovulatory phase: 20-160 pg/0.1ml; ovulatory phase: 1,000-1,700 pg/0.1ml; post ovulatory phase: 1,000-1,700 pg/0.1ml; Pregnant: 16-18 weeks: 300-800 pg/0.1ml; 28-30 weeks: 6,500-14,700 pg/0.1ml; 38-40 weeks: 12,000-19,000 pg/0.1ml. -
细胞定位
Secreted -
别名
- Pregn 4 ene 3 20 dione antibody
图片
-
Goat anti-rabbit IgG was coated onto a 96-well plate. Serial dilution of Progesterone (0, 10, 25, 50, 100, 300, 1000 pg/ml) was added (50 μl). This was followed by adding 25 μl HRP-conjugated Progesterone and 3-5 ng/ml of ab215527 (50 μl) into each well. Plates were incubated for 2 hours at room temperature by shaking. After washing, 125 μl Tetramethylbenzidine (TMB) substrate was added and incubated for 30 minutes at room temperature for color development. Then 125 ul of stopping solution (acid solution) was dispensed and OD was read at 450 nm on a microplate reader within 10 minutes.
This data was kindly provided by Pantex Division of Bio-Analysis, Inc.
-
ab215527 is specific to Progesterone and the percent cross reactivity with other compounds is demonstrated in the table.
This data was kindly provided by Pantex Division of Bio-Analysis, Inc.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
Certificate of Compliance
文献 (0)
ab215527 尚未被引用在任何文献中。