小鼠placenta normal组织裂解物- total蛋白(ab29139)
概述
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产品名称
小鼠placenta normal组织裂解物- total蛋白 -
常规说明
Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
经测试应用
适用于: WBmore details
性能
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Mycoplasma free
Yes -
形式
Liquid -
存放说明
Store at -80°C. Allow to warm to room temp and agitate gently before aliquotting. -
存储溶液
Constituents: 1% 4-Nonylphenol, branched, ethoxylated, Potassium chloride, 0.1% Bromophenol blue, Magnesium chloride, 1.2% Beta mercaptoethanol, 2% Sodium lauryl sulfate, HEPES, Sucrose, 10% Glycerol
With Protease inhibitor cocktail/EDTA/Sodium deoxycholate -
Concentration information loading... -
裂解物说明
Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot. -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab29139于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent dilution. Samples need to boiled for 5 minutes and put on ice before use.
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| 说明 |
|---|
|
WB
Use at an assay dependent dilution. Samples need to boiled for 5 minutes and put on ice before use. |
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab29139 被引用在 1 文献中.
- Meredith M et al. Growing Mouse Oocytes Transiently Activate Folate Transport via Folate Receptors As They Approach Full Size. Biol Reprod 94:125 (2016). PubMed: 27122634