小鼠eye组织裂解物- total蛋白
Mouse eye tissue lysate - total protein
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- WB
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Western blot - Mouse eye tissue lysate - total protein (AB4029)
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab114138 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
All lanes:
Western blot - Anti-Otx2 antibody (<a href='/products/primary-antibodies/otx2-antibody-ab114138'>ab114138</a>) at 1 µg/mL
Lane 1:
E10 Mouse Embryo Brain Tissue Lysate at 10 µg
Lane 2:
E14 Mouse Embryo Brain Tissue Lysate at 10 µg
Lane 3:
E16 Ms Embryo Brain Tissue Lysate at 10 µg
Lane 4:
Western blot - Mouse eye tissue lysate - total protein (ab4029) at 10 µg
Lane 5:
Y79 (Human retinoblastoma cell line) Whole Cell Lysate at 10 µg
Lane 6:
WERI (Human Retinoblastoma) Whole Cell Lysate at 10 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution
Predicted band size: 32 kDa
Observed band size: 37 kDa,62 kDa
true
Exposure time: 12min
反应性数据
产品详情
Source: Outbred NIH-Swiss Albino mice.
Mouse whole eye lysate was prepared by homogenization in modified RIPA buffer (50 mM Tris-HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecyl sulfate (SDS), 1 mM sodium ethylene diamine tetra acetate, 1 mM phenyl methyl sulfonyl flouride, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris was removed by centrifugation (12000rpm). Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% SDS, 0.01% bromophenol blue) containing 5% Beta-mercaptoethanol.
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