小鼠brain组织裂解物- total蛋白(0 days)

17 Images

• WB

Unknown

Western blot - Mouse brain tissue lysate - total protein (0 days) (AB7188)

All lanes:

Western blot - Anti-Hippocalcin antibody (<a href='/products/primary-antibodies/hippocalcin-antibody-ab24560'>ab24560</a>) at 1 µg/mL

Lane 1:

Human brain tissue lysate - total protein (<a href='/products/unavailable/human-brain-tissue-lysate-total-protein-ab29466'>ab29466</a>) at 20 µg

Lane 2:

Brain (Rat) Tissue Lysate at 20 µg

Lane 3:

Brain (Mouse) Tissue Lysate at 20 µg

Lane 4:

Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 22 kDa

Observed band size: 14 kDa,23 kDa,42 kDa,52 kDa

true

Exposure time: 4min

• WB

Ap

Western blot - Mouse brain tissue lysate - total protein (0 days) (AB7188)

All lanes:

Western blot - Anti-Torsin A/DYT1 antibody (<a href='/products/primary-antibodies/torsin-a-dyt1-antibody-ab34540'>ab34540</a>) at 1 µg/mL

Lane 1:

Marker

Lane 2:

Mouse Brain at 10 µg

Lane 3:

Testis (Mouse) Whole Cell Lysate - normal tissue at 10 µg

Lane 4:

Mouse Heart Mouse Tissue Lysate at 10 µg

Lane 5:

Western blot - Mouse skeletal muscle tissue lysate - total protein (<a href='/products/tissue-lysates/mouse-skeletal-muscle-tissue-lysate-total-protein-ab29711'>ab29711</a>) at 10 µg

Lane 6:

Mouse Fetal lysate - 14 Days old Tissue Lysate at 10 µg

Lane 7:

Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188) at 10 µg

Lane 8:

Mouse Embryonic Fibroblast 1 at 10 µg

Lane 9:

Western blot - NIH/3T3 whole cell lysate (<a href='/products/cell-lysates/nih-3t3-whole-cell-lysate-ab7179'>ab7179</a>) at 10 µg

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

Predicted band size: 38 kDa

Observed band size: 105 kDa,38 kDa,65 kDa

false

• WB

Project

Western blot - Mouse brain tissue lysate - total protein (0 days) (AB7188)

All lanes:

Western blot - Anti-Neuronatin antibody (<a href='/products/primary-antibodies/neuronatin-antibody-ab27266'>ab27266</a>) at 1 µg/mL

All lanes:

Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188) at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 9 kDa

Observed band size: 10 kDa,70-75 kDa

false

Exposure time: 5min

• WB

Project

Western blot - Mouse brain tissue lysate - total protein (0 days) (AB7188)

All lanes:

Western blot - Anti-SATB2 antibody (<a href='/products/primary-antibodies/satb2-antibody-ab34735'>ab34735</a>) at 1 µg/mL

All lanes:

Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188) at 10 µg

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

Predicted band size: 83 kDa

Observed band size: 30 kDa,85 kDa

false

• WB

Project

Western blot - Mouse brain tissue lysate - total protein (0 days) (AB7188)

We are unsure as to the biological basis of the band at 190 kDa. However, it is successfully blocked by peptide (ab39349) suggesting this may be a possible isoform of Plexin A4.

All lanes:

Western blot - Anti-Plexin A4 antibody (<a href='/products/primary-antibodies/plexin-a4-antibody-ab39350'>ab39350</a>) at 1/250 dilution

Lane 1:

Brain (Mouse) Tissue Lysate at 10 µg

Lane 2:

Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188) at 10 µg

Lane 3:

Dorsal Root Ganglia (Mouse) Tissue Lysate at 10 µg

Lane 4:

Brain (Mouse) Tissue Lysate at 10 µg with Mouse Plexin A4 peptide (<a href='/products/unavailable/mouse-plexin-a4-peptide-ab39349'>ab39349</a>)

Lane 5:

Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188) at 10 µg with Mouse Plexin A4 peptide (<a href='/products/unavailable/mouse-plexin-a4-peptide-ab39349'>ab39349</a>)

Lane 6:

Dorsal Root Ganglia (Mouse) Tissue Lysate at 10 µg with Mouse Plexin A4 peptide (<a href='/products/unavailable/mouse-plexin-a4-peptide-ab39349'>ab39349</a>)

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 212 kDa

Observed band size: 190 kDa,213 kDa,63 kDa

false

• WB

Unknown

Western blot - Mouse brain tissue lysate - total protein (0 days) (AB7188)

This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab39350 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

All lanes:

Western blot - Anti-Plexin A4 antibody (<a href='/products/primary-antibodies/plexin-a4-antibody-ab39350'>ab39350</a>) at 1 µg/mL

Lane 1:

Brain (Mouse) Tissue Lysate at 20 µg

Lane 2:

Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188) at 20 µg

Lane 3:

Brain (Rat) Tissue Lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/35000 dilution

Predicted band size: 212 kDa

Observed band size: 190 kDa,213 kDa,41 kDa,70 kDa

true

Exposure time: 8min

• WB

Project

Western blot - Mouse brain tissue lysate - total protein (0 days) (AB7188)

All lanes:

Western blot - Anti-Doublecortin antibody (<a href='/products/primary-antibodies/doublecortin-antibody-ab18723'>ab18723</a>) at 1 µg/mL

All lanes:

Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188) at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 49 kDa

Observed band size: 45 kDa,49 kDa

false

• WB

Project

Western blot - Mouse brain tissue lysate - total protein (0 days) (AB7188)

All lanes:

Western blot - Anti-SNAP25 antibody (<a href='/products/primary-antibodies/snap25-antibody-ab41726'>ab41726</a>) at 1 µg/mL

Lane 1:

Spinal Cord (Mouse) Tissue Lysate at 10 µg

Lane 2:

Spinal Cord (Rat) Tissue lysate at 10 µg

Lane 3:

Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188) at 10 µg

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 23 kDa

Observed band size: 26 kDa

false

• WB

Unknown

Western blot - Mouse brain tissue lysate - total protein (0 days) (AB7188)

All lanes:

Western blot - Anti-DIO2 antibody (<a href='/products/primary-antibodies/dio2-antibody-ab77779'>ab77779</a>) at 1 µg/mL

Lane 1:

Mouse Retina Tissue Lysate at 10 µg

Lane 2:

Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188) at 10 µg

Lane 3:

Brain (Rat) Tissue Lysate at 10 µg

Lane 4:

Human heart tissue lysate - total protein (<a href='/products/unavailable/human-heart-tissue-lysate-total-protein-ab29431'>ab29431</a>) at 10 µg

Secondary

All lanes:

Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) at 1/10000 dilution

Predicted band size: 31 kDa

Observed band size: 35 kDa,40 kDa,55 kDa

true

Exposure time: 3min

• WB

Unknown

Western blot - Mouse brain tissue lysate - total protein (0 days) (AB7188)

LRRTM1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

All lanes:

Western blot - Anti-LRRTM1 antibody (<a href='/products/primary-antibodies/lrrtm1-antibody-ab106414'>ab106414</a>) at 1/250 dilution

Lane 1:

Forebrain (Mouse) Tissue Lysate at 10 µg

Lane 2:

Mouse Hippocampus Tissue Lysate at 10 µg

Lane 3:

Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188) at 10 µg

Lane 4:

Mouse Olfactory Bulb Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 58 kDa

Observed band size: 130 kDa,30 kDa,40 kDa,65 kDa

true

Exposure time: 8min

• WB

Project

Western blot - Mouse brain tissue lysate - total protein (0 days) (AB7188)

ab35448 detects NR2D at the predicted MW of 143 kDa consistent with published data on NR2D protein (Dunah et al, 1996; 1998); we are unsure as to the identity of the lower bands.

All lanes:

Western blot - Anti-GluN2D antibody (<a href='/products/primary-antibodies/glun2d-antibody-ab35448'>ab35448</a>) at 1 µg/mL

Lane 1:

Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188) at 20 µg

Lane 2:

Brain (Mouse) Tissue Lysate, 7 days old at 20 µg

Lane 3:

Brain (Rat) Tissue Lysate, 0 Days old at 20 µg

Lane 4:

Brain (Rat) Tissue Lysate, 7 days old at 20 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 144 kDa

Observed band size: 125 kDa,143 kDa,45 kDa,60 kDa,~200 kDa

false

Exposure time: 20min

• WB

Project

Western blot - Mouse brain tissue lysate - total protein (0 days) (AB7188)

Rabbit polyclonal to Lingo1 (ab23631) detects Lingo1 protein at ~83kDa in mouse and human brain lysates. This band is larger than predicted on Swiss Prot (69kDa; Q9D1T0) possibly due to post-translational modifications and is consistent with published literature on Lingo1 protein detection in brain lysate. The strong band observed at ~ 17kDa in the mouse brain lysate (lane 1) corresponds to a cleavage fragment of Lingo1 (Swiss Prot IDs : Q3TQJ4)

All lanes:

Western blot - Anti-Lingo1 antibody (<a href='/products/primary-antibodies/lingo1-antibody-ab23631'>ab23631</a>) at 1 µg/mL

Lane 1:

Mouse brain at 20 µg

Lane 2:

Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188) at 20 µg

Lane 3:

Human Brain Tissue Lysate at 20 µg

Secondary

All lanes:

Rabbit IgG secondary antibody (ab28446) at 1/10000 dilution

Predicted band size: 70 kDa

Observed band size: 17 kDa,34 kDa,83 kDa

false

• WB

Project

Western blot - Mouse brain tissue lysate - total protein (0 days) (AB7188)

All lanes:

Western blot - Anti-BLBP antibody (<a href='/products/primary-antibodies/blbp-antibody-ab32423'>ab32423</a>) at 1/250 dilution

All lanes:

Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188) at 10 µg

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 14 kDa

Observed band size: 15 kDa

false

• WB

Project

Western blot - Mouse brain tissue lysate - total protein (0 days) (AB7188)

Plexin A2 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

All lanes:

Western blot - Anti-Plexin A2 antibody (<a href='/products/primary-antibodies/plexin-a2-antibody-ab39357'>ab39357</a>) at 2 µg/mL

All lanes:

Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188) at 20 µg

Secondary

All lanes:

Goat Polyclonal to rabbit IgG - H&L - Pre adsorbed (HRP) at 1/3000 dilution

Predicted band size: 211 kDa

Observed band size: 230 kDa

false

• WB

Ap

Western blot - Mouse brain tissue lysate - total protein (0 days) (AB7188)

All lanes:

Western blot - Anti-Neuronatin antibody (<a href='/products/primary-antibodies/neuronatin-antibody-ab27266'>ab27266</a>) at 1 µg/mL

All lanes:

Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188) at 20 µg

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

Predicted band size: 9 kDa

false

• WB

Project

Western blot - Mouse brain tissue lysate - total protein (0 days) (AB7188)

Lane 1:

Marker

Lanes 2 - 4:

Western blot - Anti-beta Arrestin 1 antibody (<a href='/products/primary-antibodies/beta-arrestin-1-antibody-ab31868'>ab31868</a>) at 1 µg/mL

Lane 2:

Mouse Brain at 20 µg

Lane 3:

Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188) at 20 µg

Lane 4:

Brain (Rat) Whole Cell Lysate - normal tissue at 20 µg

Secondary

All lanes:

IR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

Predicted band size: 47 kDa

Observed band size: 100 kDa,50 kDa

false

• WB

Project

Western blot - Mouse brain tissue lysate - total protein (0 days) (AB7188)

All lanes:

Western blot - Anti-CAPS1 antibody (<a href='/products/primary-antibodies/caps1-antibody-ab32011'>ab32011</a>) at 1 µg/mL

Lane 1:

Brain (Rat) Tissue Lysate at 10 µg

Lane 2:

Brain (Rat) Tissue Lysate (<a href='/products/unavailable/brain-rat-tissue-lysate-ab7942'>ab7942</a>)Brain (Rat) Tissue Lysate (<a href='/products/unavailable/brain-rat-tissue-lysate-ab7942'>ab7942</a>) at 10 µg

Lane 3:

Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188) at 10 µg

Lane 3:

Heart (Mouse) Tissue Lysate at 10 µg

Lane 4:

Mouse Fetus (14 Day Old) Tissue Lysate (ab46769)Mouse Fetus (14 Day Old) Tissue Lysate (ab46769) at 10 µg with CAPS1 peptide (ab32785)CAPS1 peptide (ab32785)

Lane 5:

MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab46770)MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab46770) at 10 µg with CAPS1 peptide (ab32785)CAPS1 peptide (ab32785)

Lane 6:

Spinal Cord (Mouse) Tissue Lysate (ab50253)Spinal Cord (Mouse) Tissue Lysate (ab50253) at 10 µg with CAPS1 peptide (ab32785)CAPS1 peptide (ab32785)

Lane 7:

Spinal Cord (Rat) Tissue Lysate (ab50252)Spinal Cord (Rat) Tissue Lysate (ab50252) at 10 µg

Lane 8:

Hippocampus (Mouse) Tissue Lysate (ab50259)Hippocampus (Mouse) Tissue Lysate (ab50259) at 10 µg

Secondary

All lanes:

Rabbit IgG secondary antibody (ab28446)Rabbit IgG secondary antibody (ab28446) at 1/10000 dilution

Predicted band size: 153 kDa

Observed band size: 146 kDa,55 kDa

false