Prestained蛋白Ladder - Mid-range molecular weight (10 - 180 kDa) (ab116027)
概述
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产品名称
Prestained蛋白Ladder - Mid-range molecular weight (10 - 180 kDa)
参阅全部 Prestained Protein Ladder 试剂 -
经测试应用
适用于: WB, SDS-PAGEmore details -
常规说明
Prestained Protein Ladder ab116027 is a three-color protein standard with 10 pre-stained proteins covering a wide range molecular weights for 10 to 180 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer).
This product was previously called Prism Ultra Protein Ladder (10 - 180 kDa).
This prestained protein ladder is designed for monitoring protein separated during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximate sizing of proteins. The ladder is supplied in gel loading buffer and is ready to use.
Do not heat, dilute, add reducing agent before loading.
Key product features:
- Broad range: 10-180 kDa
- Ready-to-use: Supplied in a loading buffer for direct loading on gels.
- Easy to identify: Includes green ~25 kDa and red ~75kDa reference bands.
- Sharp bands
- Recommended loading: ~2 - 3 µl for western blots
Review other protein ladders in the unstained and prestained protein ladder guide.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
存储溶液
pH: 7.50
Constituents: 0.02% Urea, 2% Sodium lauryl sulfate, 3% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 15% Glycerol (glycerin, glycerine), 1% Tris phosphate -
Concentration information loading... -
研究领域
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别名
- Molecular weight marker
- Prism protein ladder
- SDS PAGE protein ladder prestained
see all
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab116027于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration.
~2 - 3 µl per well for general Western transfering. Apply more for thicker (>1.5 mm) or larger gel. |
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| SDS-PAGE |
Use at an assay dependent concentration. 3 µl or 5 µl per loading for clear visualization during electrophoresis
on 15-well or 10-well mini-gel, respectively.
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| 说明 |
|---|
|
WB
Use at an assay dependent concentration. ~2 - 3 µl per well for general Western transfering. Apply more for thicker (>1.5 mm) or larger gel. |
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SDS-PAGE
Use at an assay dependent concentration. 3 µl or 5 µl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. |
图片
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SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa) (ab116027)Gel 1: Tris-Glycine (~4-20%) Gel 2: Bis-Tris (12%) MOPS buffer Gel 3: Bis-Tris (10%) MES buffer Pre-stained molecular weight standards have a differing mobility and as a consequence varying apparent molecular weight when run in distinct SDS-PAGE buffer systems. The variance in pH of alternative buffers affects the charge of the labelled protein standard and its binding capacity for SDS. The apparent molecular weight of this marker has been determined by calibration against an unstained ladder in each electrophoresis condition.
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Western blot - Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa) (ab116027)This image is courtesy of an anonymous Abreview.Very easy to use protein ladder in the range from 10-180 kDa. Good separation in SDS-PAGE and nice transfer onto nitrocellulose membrane. The gel used was a 10% Tris-Bis gel from life-technologies. the protein ladder was used at 2.5µl per well.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab116027 被引用在 5 文献中.
- Burkitbayev M et al. Effect of sulfur-containing agrochemicals on growth, yield, and protein content of soybeans (Glycine max (L.) Merr). Saudi J Biol Sci 28:891-900 (2021). PubMed: 33424381
- Nikooie R et al. Lactate regulates autophagy through ROS-mediated activation of ERK1/2/m-TOR/p-70S6K pathway in skeletal muscle. J Cell Commun Signal 15:107-123 (2021). PubMed: 33398722
- Shadman Z et al. Isolation and characterizations of a novel recombinant scFv antibody against exotoxin A of Pseudomonas aeruginosa. BMC Infect Dis 21:300 (2021). PubMed: 33761869
- Bielecka ZF et al. Hypoxic 3D in vitro culture models reveal distinct resistance processes to TKIs in renal cancer cells. Cell Biosci 7:71 (2017). PubMed: 29270287
- Fiserova J et al. Entry into the nuclear pore complex is controlled by a cytoplasmic exclusion zone containing dynamic GLFG-repeat nucleoporin domains. J Cell Sci 127:124-36 (2014). PubMed: 24144701