Prestained Protein Ladder - Extra broad molecular weight (6.5 - 270 kDa)
Prestained Protein Ladder - Extra broad molecular weight (6.5 - 270 kDa)
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- Suitable for western blot (WB) and SDS-PAGE
- Suitable for Tris Glycine (4-12%) SDS buffer, Bis-Tris (4-12%) MOPS buffer and Bis-Tris (4-12%) MES buffer
- Part of our Lab essentials range western blot workflow
- SDS-PAGE
Supplier Data
SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (6.5 - 270 kDa) (AB234592)
SDS-PAGE analysis with ab234592.
Gel 1 : Tris-Glycine 4-20%
Gel 2 : Bis-Tris 4-12% MES buffer.
The apparent molecular weight (kDa) of each protein has been determined by calibration against unstained protein standards; supplemental data should be considered for more accurate adjustment in different electrophoresis conditions.
反应性数据
产品详情
Prestained Protein Ladder ab234592 is a three-color protein standard with 10 pre-stained proteins covering a range of MW from 6.5-270 kDa. This prestained protein ladder allows you to monitor protein separation during SDS-polyacrylamide gel electrophoresis and verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.
Key product features:
- Broad range: 6.5-270 kDa
- Ready to use: The ladder is supplied in the gel loading buffer and is ready to use.
- Easily identified: Proteins are covalently coupled with a blue chromophore except three reference bands (two orange bands at 30 kDa and 270 kDa and one green band at 52 kDa).
3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring.
Review other protein ladders in the unstained and prestained protein ladder guide.
性能和储存信息
运输条件
推荐的短期储存时间
推荐的短期储存条件
推荐的长期储存条件
靶点信息
文献 (1)
Recent publications for all applications. Explore the full list and refine your search
Scientific reports 10:14724 PubMed32895418
2020
Applications
Unspecified application
Species
Unspecified reactive species
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