羊抗兔IgG H&L (HRP) (ab6795)

Submit a review Q&A (1)References (22)
IHC - Wholemount - Sheep Anti-Rabbit IgG H&L (HRP) (ab6795)
  • Western blot - Sheep Anti-Rabbit IgG H&L (HRP) (ab6795)

Key features and details

  • Sheep Anti-Rabbit IgG H&L (HRP)
  • Conjugation: HRP
  • Host species: Sheep
  • Suitable for: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Fr

Conjugates logo Related conjugates and formulations

DyLight® 488 DyLight® 650 FITC Texas Red ® TRITC Unconjugated

概述

  • 产品名称

    羊抗兔IgG H&L (HRP)
    参阅全部 IgG 二抗

  • 宿主

    Sheep

  • 靶标种属

    Rabbit

  • 经测试应用

    适用于: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Frmore details

  • 免疫原

    Rabbit IgG, whole molecule

  • 偶联物

    HRP

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C.

  • 存储溶液

    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...

  • 纯度

    Affinity purified

  • 纯化说明

    Horseradish Peroxidase (HRP).

  • 共轭说明

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads

  • 克隆

    多克隆

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab6795于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use at an assay dependent dilution.
ELISA
Use at an assay dependent dilution.
Immunomicroscopy
Use at an assay dependent concentration.
Dot blot
Use at an assay dependent concentration.
ICC/IF
1/500 - 1/2500.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
说明
WB
Use at an assay dependent dilution.
ELISA
Use at an assay dependent dilution.
Immunomicroscopy
Use at an assay dependent concentration.
Dot blot
Use at an assay dependent concentration.
ICC/IF
1/500 - 1/2500.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.

图片

  • IHC - Wholemount - Sheep Anti-Rabbit IgG H&L (HRP) (ab6795)
    IHC - Wholemount - Sheep Anti-Rabbit IgG H&L (HRP) (ab6795)This image is courtesy of an Abreview submitted by Michal Szlis
  • Western blot - Sheep Anti-Rabbit IgG H&L (HRP) (ab6795)
    Western blot - Sheep Anti-Rabbit IgG H&L (HRP) (ab6795)

    ab6795 was used at dilution 1/2000 with the primary antibody ab8895 in WB. See the review on ab8895.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (22)

发表研究结果有使用 ab6795？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab6795 被引用在 22 文献中.

  • Afewerky HK  et al. Sodium-calcium exchanger isoform-3 targeted Withania somnifera (L.) Dunal therapeutic intervention ameliorates cognition in the 5xFAD mouse model of Alzheimer's disease. Sci Rep 12:1537 (2022). PubMed: 35087161
  • Peng L  et al. Transcription factor SNAI2 exerts pro-tumorigenic effects on glioma stem cells via PHLPP2-mediated Akt pathway. Cell Death Dis 13:516 (2022). PubMed: 35654777
  • Jiang H  et al. CART mitigates oxidative stress and DNA damage in memory deficits of APP/PS1 mice via upregulating ß-amyloid metabolism-associated enzymes. Mol Med Rep 23:N/A (2021). PubMed: 33604684
  • Wirasinha RC  et al. Nfkb2 variants reveal a p100-degradation threshold that defines autoimmune susceptibility. J Exp Med 218:N/A (2021). PubMed: 33107914
  • Luo R  et al. Long non-coding RNA MEG3 inhibits apoptosis of retinal pigment epithelium cells induced by high glucose via the miR-93/Nrf2 axis. Am J Pathol N/A:N/A (2020). PubMed: 32473920
View all Publications for this product

客户评价及客户问答

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Question

Hi Abcam,

I tried contacting the abcam distributer here in Australia (Sapphire bioscience), but the representative was unable to answer my questions.

I would like to purchase one secondary antibody against primary abcam antibodies I already have.

I would like to use the antibodies in a dot blot assay, using the ECL detection method.

There are three possible secondary antibodies that seem to me like they will work for two of my primaries:

Primary: ab8898 and ab9051
Secondary: ab97064, ab97095 and ab97200

Which secondary should I choose that will be best for both of my primaries, using ECL detection in a Dot Blot assay?

Thank you in advance for your assistance.

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Answer

Thank you for contacting us.

The secondary antibodies you mentioned might work for dot blots with the rabbit polyclonal Histone primaries, as they are guaranteed to work in Western blot which is quite similar to dot blot. However, we have not tested them specifically for this application and thus cannot guarantee that it would work.

Therefore, I would like to recommend one of the following secondaries which have been tested in dot blot:

ab6721: https://www.abcam.com/Goat-Rabbit-IgG-HL-HRP-ab6721.html (or use the following: https://www.abcam.com/Goat-Rabbit-IgG-HL-HRP-ab6721.html).

ab6802: https://www.abcam.com/Donkey-Rabbit-IgG-HL-HRP-ab6802.html (or use the following: https://www.abcam.com/Donkey-Rabbit-IgG-HL-HRP-ab6802.html).

ab6795: https://www.abcam.com/Sheep-Rabbit-IgG-HL-HRP-ab6795.html (or use the following: https://www.abcam.com/Sheep-Rabbit-IgG-HL-HRP-ab6795.html).

They are raised in goat, donkey and sheep and apart from this are equally well suited for your experimental setup. If you need a decision guiding you could for example choose the one with the most references: this would be ab6721 with 52 publications mentioning the use of this antibody.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase (https://www.abcam.com/Abpromise).

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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