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羊抗小鼠IgG H&L (ab6710)

Submit a review Q&A (2)References (1)

Key features and details

  • Sheep Anti-Mouse IgG H&L
  • Host species: Sheep
  • Suitable for: WB, ELISA, IP, Immunodiffusion, Conjugation, ICC/IF, IHC-P, IHC-Fr

Conjugates logo Related conjugates and formulations

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概述

  • 产品名称

    羊抗小鼠IgG H&L
    参阅全部 IgG 二抗

  • 宿主

    Sheep

  • 靶标种属

    Mouse

  • 经测试应用

    适用于: WB, ELISA, IP, Immunodiffusion, Conjugation, ICC/IF, IHC-P, IHC-Frmore details

  • 免疫原

    Mouse IgG whole molecule

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...

  • 纯度

    Affinity purified

  • 纯化说明

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

  • 克隆

    多克隆

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab6710于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/2000 - 1/10000.
ELISA
1/100000.
IP
Use at an assay dependent concentration.
Immunodiffusion
Use at an assay dependent concentration.
Conjugation
Use at an assay dependent concentration.
ICC/IF
1/1000 - 1/5000.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
说明
WB
1/2000 - 1/10000.
ELISA
1/100000.
IP
Use at an assay dependent concentration.
Immunodiffusion
Use at an assay dependent concentration.
Conjugation
Use at an assay dependent concentration.
ICC/IF
1/1000 - 1/5000.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (1)

发表研究结果有使用 ab6710？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab6710 被引用在 1 文献中.

  • Feng X  et al. Transplanted embryonic retinal stem cells have the potential to repair the injured retina in mice. BMC Ophthalmol 21:26 (2021). PubMed: 33422026

客户评价及客户问答

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1-2 of 2 Abreviews or Q&A

Question

what is the molecular weight of such polyclonal antibody (Sheep anti-Mouse Ig)? Do the dilution for this antibody in radiodetection experiment (radiolabelled antibody) is less or more than the one for immunochemistry?

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Answer

This antibody is approximately 150 kDa in weight. Dilutions in a radiodetection experiment are usually higher than for IHC

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Question

Is there a sheep or goat anti-mouse IgG1- or Ig-Fc polyclonal available currently? Jim

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Answer

No, I'm afraid that we do not stock these products

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