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大鼠单克隆抗体[SB84a]抗小鼠IgG2a heavy chain (Alexa Fluor® 488) (ab172324)

Reviews (1) Submit a question References (2)
Flow Cytometry - Rat monoclonal [SB84a] Anti-Mouse IgG2a heavy chain (Alexa Fluor® 488) (ab172324)
  • Alexa Fluor® - Rat monoclonal [SB84a] Anti-Mouse IgG2a heavy chain (Alexa Fluor® 488) (ab172324)

Key features and details

  • Rat monoclonal [SB84a] Anti-Mouse IgG2a heavy chain (Alexa Fluor® 488)
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Host species: Rat
  • Isotype: IgG1
  • Suitable for: IHC-Fr, Flow Cyt

概述

  • 产品名称

    大鼠单克隆抗体[SB84a]抗小鼠IgG2a heavy chain (Alexa Fluor® 488)
    参阅全部 IgG2a 二抗

  • 宿主

    Rat

  • 靶标种属

    Mouse

  • 特异性

    This antibody is specific to Mouse IgG2a heavy chain

  • 经测试应用

    适用于: IHC-Fr, Flow Cytmore details

  • 免疫原

    The details of the immunogen for this antibody are not available.

  • 偶联物

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.

  • 存储溶液

    Preservative: 0.02% Sodium azide
    Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA
  • Concentration information loading...

  • 纯度

    Immunogen affinity purified

  • 克隆

    单克隆

  • 克隆编号

    SB84a

  • 同种型

    IgG1

  • 轻链类型

    kappa

  • 常规说明

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab172324于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-Fr
Use at an assay dependent concentration.
Flow Cyt
1/1000 - 1/2000.

ab171464 - Mouse monoclonal IgG2a (Alexa Fluor® 488), is suitable for use as an isotype control to complement this secondary antibody.
说明
IHC-Fr
Use at an assay dependent concentration.
Flow Cyt
1/1000 - 1/2000.

ab171464 - Mouse monoclonal IgG2a (Alexa Fluor® 488), is suitable for use as an isotype control to complement this secondary antibody.

图片

  • Flow Cytometry - Rat monoclonal [SB84a] Anti-Mouse IgG2a heavy chain (Alexa Fluor® 488) (ab172324)
    Flow Cytometry - Rat monoclonal [SB84a] Anti-Mouse IgG2a heavy chain (Alexa Fluor® 488) (ab172324)

    Overlay histogram showing subclass specificity of ab172324. HeLa cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% serum / 0.3M glycine to block non-specific protein-protein interactions followed by the primary antibody (1μg/1x106 cells) as indicated: ab8982 (Mouse IgG1), ab49105 (Mouse IgG2a), ab16965 (Mouse IgG2b), ab60602 (Mouse IgG3), ab55602 (Mouse IgM) for 30 min at 22°C. The secondary antibody (ab172324 - Rat monoclonal (SB84a) Anti-Mouse IgG2a, heavy chain - Alexa Fluor® 488) was used at 1/1000 dilution for 30 min at 22ºC. Unlabelled (blue line) and secondary only (gold line) samples were also used as negative controls. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • Alexa Fluor® - Rat monoclonal [SB84a] Anti-Mouse IgG2a heavy chain (Alexa Fluor® 488) (ab172324)
    Alexa Fluor® - Rat monoclonal [SB84a] Anti-Mouse IgG2a heavy chain (Alexa Fluor® 488) (ab172324)

    Emission spectra of Alexa Fluor® fluorochromes available in our catalog. Line colors represent the approximate visible colors of the wavelength maxima.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (2)

发表研究结果有使用 ab172324？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab172324 被引用在 2 文献中.

  • Gao Y  et al. Effects of pyrroloquinoline quinone on noise-induced and age-related hearing loss in mice. Sci Rep 12:15911 (2022). PubMed: 36151123
  • Khosravi-Maharlooei M  et al. Role of the thymus in spontaneous development of a multi-organ autoimmune disease in human immune system mice. J Autoimmun 119:102612 (2021). PubMed: 33611150

客户评价及客户问答

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ab172324 - Rat monoclonal (SB84a) Anti-Mouse IgG2a heavy chain (Alexa Fluor® 488)

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Protocol:
1. Tissue was fixed in cold acetone for 10 minutes
2. Tissue was blocked with 1% BSA, 0.1% in PBS (Blocking Buffer) for 30 minutes
3. Tissue was stained with first antibody pure mouse-anti-human HLA-DR overnight in Blocking Buffer
4. After washing, Tissue was stained with ab172324 - Rat monoclonal (SB84a) Anti-Mouse IgG2a heavy chain (Alexa Fluor® 488) for 2 hours in Blocking Buffer.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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提交于 Jun 18 2016

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