大鼠单克隆抗体[SB76b]抗小鼠IgG3 gamma chain (Alexa Fluor® 647) (ab172329)
![Flow Cytometry - Rat monoclonal [SB76b] Anti-Mouse IgG3 gamma chain (Alexa Fluor® 647) (ab172329)](https://www.abcam.cn/ps/products/172/ab172329/Images/ab172329-226310-rat-monoclonal-sb76b-anti-mouse-igg3-gamma-chain-alexa-fluor-647-flow-cytometry.jpg "Flow Cytometry - Rat monoclonal [SB76b] Anti-Mouse IgG3 gamma chain (Alexa Fluor® 647) (ab172329)")
Key features and details
- Rat monoclonal [SB76b] Anti-Mouse IgG3 gamma chain (Alexa Fluor® 647)
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
- Host species: Rat
- Isotype: IgG3
- Suitable for: Flow Cyt, IHC-P, ELISA, ICC/IF
概述
-
产品名称
大鼠单克隆抗体[SB76b]抗小鼠IgG3 gamma chain (Alexa Fluor® 647)
参阅全部 IgG3 二抗 -
宿主
Rat -
靶标种属
Mouse -
特异性
Reacts with mouse IgG3 as demonstrated by ELISA. Minimal reaction with other mouse isotypes. May react with IgG from other species. -
经测试应用
适用于: Flow Cyt, IHC-P, ELISA, ICC/IFmore details -
免疫原
The details of the immunogen for this antibody are not available.
-
偶联物
Alexa Fluor® 647. Ex: 652nm, Em: 668nm
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
存储溶液
Preservative: 0.02% Sodium azide
Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading... -
克隆
单克隆 -
克隆编号
SB76b -
同种型
IgG3 -
常规说明
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
-
研究领域
相关产品
-
Alternative Versions
-
Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab172329于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
1/3000.
|
|
| IHC-P |
Use at an assay dependent concentration.
|
|
| ELISA |
Use at an assay dependent concentration.
|
|
| ICC/IF |
1/200 - 1/1000.
|
| 说明 |
|---|
|
Flow Cyt
1/3000. |
|
IHC-P
Use at an assay dependent concentration. |
|
ELISA
Use at an assay dependent concentration. |
|
ICC/IF
1/200 - 1/1000. |
图片
-
Flow Cytometry - Rat monoclonal [SB76b] Anti-Mouse IgG3 gamma chain (Alexa Fluor® 647) (ab172329)
Overlay histogram showing subclass specificity of ab172329. HeLa cells were fixed with 80% methanol (-20ºC, 5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% serum / 0.3M glycine to block non-specific protein-protein interactions followed by the primary antibody (1μg/1x106 cells) as indicated: ab8982 (Mouse IgG1), ab49105 (Mouse IgG2a), ab16965 (Mouse IgG2b), ab60602 (Mouse IgG3), ab55602 (Mouse IgM) for 30 min at 22ºC. The secondary antibody (ab172329 - Rat monoclonal (SB76b) Anti-Mouse IgG3 gamma chain - Alexa Fluor® 647) was used at 1/3000 dilution for 30 min at 22ºC. Unlabelled (blue line) and secondary only (black line) samples were also used as negative controls. Acquisition of >5,000 events were collected using a solid-state 25mW red diode laser (635nm) and 675/30 bandpass filter.
-
![Alexa Fluor® - Rat monoclonal [SB76b] Anti-Mouse IgG3 gamma chain (Alexa Fluor® 647) (ab172329)](https://www.abcam.cn/ps/products/172/ab172329/Images/ab172329-210594-rat-monoclonal-sb76b-anti-mouse-igg3-gamma-chain-alexa-fluor-647-alexa-fluor.jpg)
Alexa Fluor® - Rat monoclonal [SB76b] Anti-Mouse IgG3 gamma chain (Alexa Fluor® 647) (ab172329)Emission spectra of Alexa Fluor® fluorochromes available in our catalog. Line colors represent the approximate visible colors of the wavelength maxima.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab172329 尚未被引用在任何文献中。