大鼠单克隆抗体[SB76b]抗小鼠IgG3 gamma chain (Alexa Fluor® 488)
Rat monoclonal [SB76b] Anti-Mouse IgG3 gamma chain (Alexa Fluor® 488)
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(1 Publication)
Suitable for ELISA, IHC-P, ICC/IF, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Cited in 1 publication.
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Ig gamma-3 chain C region
- Flow Cyt
Unknown
Flow Cytometry - Rat monoclonal [SB76b] Anti-Mouse IgG3 gamma chain (Alexa Fluor® 488) (AB172328)
Overlay histogram showing subclass specificity of ab172328. HeLa cells were fixed with 80% methanol (-20°C, 5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% serum / 0.3M glycine to block non-specific protein-protein interactions followed by the primary antibody (1μg/1x106 cells) as indicated : ab8982 (Mouse IgG1), ab49105 (Mouse IgG2a), ab16965 (Mouse IgG2b), ab60602 (Mouse IgG3), ab55602 (Mouse IgM) for 30 min at 22°C. The secondary antibody (ab172328 - Rat monoclonal (SB76b) Anti-Mouse IgG3 gamma chain - Alexa Fluor® 488) was used at 1/3000 dilution for 30 min at 22°C. Unlabelled (blue line) and secondary only (black line) samples were also used as negative controls. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
- Alexa Fluor®
Supplier Data
Alexa Fluor® - Rat monoclonal [SB76b] Anti-Mouse IgG3 gamma chain (Alexa Fluor® 488) (AB172328)
Emission spectra of Alexa Fluor® fluorochromes available in our catalog. Line colors represent the approximate visible colors of the wavelength maxima.
不同偶联物与剂型 (1)
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665 Alexa Fluor® 647
Rat monoclonal [SB76b] Anti-Mouse IgG3 gamma chain (Alexa Fluor® 647)
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The Journal of experimental medicine 219: PubMed35139155
2022
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