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大鼠单克隆抗体[LO-MG2b-1]抗小鼠IgG2b heavy chain (ab11566)

Submit a review Q&A (1)References (1)

Key features and details

  • Rat monoclonal [LO-MG2b-1] Anti-Mouse IgG2b heavy chain
  • Host species: Rat
  • Isotype: IgG1
  • Suitable for: ELISA

概述

  • 产品名称

    大鼠单克隆抗体[LO-MG2b-1]抗小鼠IgG2b heavy chain
    参阅全部 IgG2b 二抗

  • 宿主

    Rat

  • 靶标种属

    Mouse

  • 特异性

    ab11566 recognises murine IgG2b, binding to the gamma 2b heavy chain.

  • 经测试应用

    适用于: ELISAmore details

  • 免疫原

    Full length protein corresponding to Mouse IgG2b. Polyclonal IgG from BALB/c mice.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 纯度

    Protein G purified

  • 纯化说明

    Purified IgG prepared by affinity chromatography.

  • 克隆

    单克隆

  • 克隆编号

    LO-MG2b-1

  • 骨髓瘤

    IR983F

  • 同种型

    IgG1

  • 常规说明

    This product should be stored undiluted. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab11566于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ELISA
说明
应用说明
ELISA (coating): Use at a concentration of 5 µg/ml.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (1)

发表研究结果有使用 ab11566？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab11566 被引用在 1 文献中.

  • Lau YF  et al. The magnitude of local immunity in the lungs of mice induced by live attenuated influenza vaccines is determined by local viral replication and induction of cytokines. J Virol 85:76-85 (2011). PubMed: 20962087

客户评价及客户问答

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Question

Hi,   I purchased a pair of antibodies to detect mouse IgG2b by sandwich assays. The antibody that I have for the capture probe is ab11566 (Rat monoclonal [LO-MG2b-1]). And I have ab99640 (Rat monoclonal [LO-MG2b-2], biotin) as the detection probe. As proof of concept, I’d like to detect known isotype control samples using the pair of antibodies that I have. Now, I’m interested in ab18421 ([MG2b-57]) and ab18469 ([MPC-11]). Which one do you recommend? Are both isotype control samples recognized by the antibodies that I have? Another question I’d like to ask is what is the meaning of clone numbers like MG2b-1 and MG2b-2? Can I know the epitope information from these numbers? Thank you.

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Answer

Thank you for your enquiry.


Both you capture and detection antibodies should recognize these isotype controls. However, as these products have not been tested in sandwich ELISA we do not know whether the capture antibody will recognize the same site or otherwise interfere with the detection antibody. Although we produce many in-house antibodies, we obtain other antibodies from a wide range of sources. Therefore, comparison experiments will not often have been done. As these products have not been tested in this application and they have not been tested against one anotherI cannot recommend one isotype control over the other.

Regarding the clone numbers, these are created by the originator the antibody for identification purposes. The epitopes of these products have not been mapped to our knowledge.



I look forward to hearing from you. Should you have any further questions please do not hesitate to ask

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