为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    按产品类型
    蛋白质与肽
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 定制化产品 & 合作关系
    定制化产品 & 合作关系

    定制化产品和商务合作加速您的诊断和治疗项目

    定制化产品

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

Immunology Immunoglobulins Heavy Chain IgG

兔抗羊IgG H&L (FITC) (ab6743)

  • Datasheet
  • SDS
Reviews (1)Q&A (5)References (11)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

运输费用

Promotion Information

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Key features and details

  • Rabbit Anti-Sheep IgG H&L (FITC)
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Host species: Rabbit
  • Suitable for: Dot blot, Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISA

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 750 Alkaline Phosphatase Biotin Glucose Oxidase Gold 10nm Gold 15nm HRP Texas Red ® TRITC Unconjugated Unconjugated

你可能会感兴趣

封闭
Normal Rabbit Serum (ab166640)
偶联
Product image
Alexa Fluor® 647 Conjugation Kit (Fast) - Lightning-Link® (ab269823)
封闭
Product image
Normal Donkey Serum (ab7475)

查看更多相关产品

概述

  • 产品名称

    兔抗羊IgG H&L (FITC)
    参阅全部 IgG 二抗
  • 宿主

    Rabbit
  • 靶标种属

    Sheep
  • 经测试应用

    适用于: Dot blot, Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISAmore details
  • 免疫原

    Sheep whole molecule

  • 偶联物

    FITC. Ex: 493nm, Em: 528nm

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C.
  • 存储溶液

    Preservative: 0.01% Sodium azide
    Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride
  • Concentration information loading...
  • 纯度

    Affinity purified
  • 纯化说明

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • 克隆

    多克隆
  • 常规说明

    Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.3 moles DTAF per mole of Rabbit IgG.

  • 研究领域

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Sheep
    • IgG
    • Fluorophore
    • FITC

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab6743于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Dot blot
Use at an assay dependent concentration.
Immunomicroscopy
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
ICC/IF
1/1000 - 1/5000.
IHC-P (1)
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ELISA
1/10000 - 1/50000.
说明
Dot blot
Use at an assay dependent concentration.
Immunomicroscopy
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
ICC/IF
1/1000 - 1/5000.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ELISA
1/10000 - 1/50000.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (11)

发表研究结果有使用 ab6743?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab6743 被引用在 11 文献中.

  • Zhou QL  et al. FPR1 gene silencing suppresses cardiomyocyte apoptosis and ventricular remodeling in rats with ischemia/reperfusion injury through the inhibition of MAPK signaling pathway. Exp Cell Res 370:506-518 (2018). PubMed: 30031130
  • Mendoza JL  et al. The IFN-?-IFN-?R1-IL-10Rß Complex Reveals Structural Features Underlying Type III IFN Functional Plasticity. Immunity 46:379-392 (2017). PubMed: 28329704
  • Tang J  et al. Construction of a general albumin promoter reporter system for real-time monitoring of the differentiation status of functional hepatocytes from stem cells in mouse, rat and human. Biomed Rep 6:627-632 (2017). PubMed: 28584633
  • Wilding C  et al. ?-Synuclein antibodies have neuroprotective potential on neuroretinal cells via proteins of the mitochondrial apoptosis pathway. PLoS One 9:e90737 (2014). PubMed: 24595072
  • Nguyen MD  et al. Cardiac cell culture model as a left ventricle mimic for cardiac tissue generation. Anal Chem 85:8773-9 (2013). PubMed: 23952579
View all Publications for this product

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

1-6 of 6 Abreviews or Q&A

For IHC-P

Excellent
Abreviews
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
I tried 1:100 and 1:500 dilutions for this antibody. 1:500 works well with an hour incubation at room temperature.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Aug 20 2014

Question

Please let me know the molecular weight of those antibodies.

Read More

Abcam community

Verified customer

Asked on Jun 29 2012

Answer

Thank you for your inquiry.

Unfortunately, we do not determine the exact molecular weight for our antibodies.

https://www.abcam.com/ab6743: asshown on the datasheet,the fluorochrome/Protein Ratio is2.3 moles DTAF per mole of Rabbit IgG.

The molecular weight of DTAF is 495 g/mol and the molecular weight of an IgG moleculeis about 150 kDa. The molecular weight of one molecule of ab6743 is therefore about 151kDa.(=2.3x495+150000).

I hope this is helpful.

Read More

Abcam Scientific Support

回复于 Jun 29 2012

Question

Finalmente como abes he comprado ab8940 anti-human serum albumin

y ab6743 anti sheep, la primera prueba no ha sido demasiado exitosa,

por lo que quería preguntar, si teneis algun protocolo de refencia.

Muchas gracias por vuestra atención

Read More

Abcam community

Verified customer

Asked on Feb 29 2012

Answer

Gracias por tu respuesta.

No tenemos un protocolo concreto para el uso de este anticuerpo mediante immunohistoquímica, sino protocolos generales que pueden servir de guía para los distintos ensayos.

Te adjunto el link del protocolo de immunohistoquímica para secciones incluidas en parafina:

https://www.abcam.com/index.html?pageconfig=resource&rid=11384

Así como el “recetario” de los distintos pasos a seguir:

https://www.abcam.com/index.html?pageconfig=resource&rid=13046

También te pueden resultar útiles los consejos para tratar los posibles problemas que pueden darse a lo largo del ensayo:

https://www.abcam.com/index.html?pageconfig=resource&rid=11467

Las únicas recomendaciones concretas para el uso de estos anticuerpos es utilizar como agente de bloqueo suero de conejo (especie de procedencia del secundario) y usar el primario a una dilución de 1/1000. Quizás te ayude echar un vistazo al Abreview publicado por otro usuario del anticuerpo ab8940 para comprobar en qué condiciones usó el anticuerpo.

Es importante también usar siempre controles positivos para ayudar a determinar el origen de los posibles problemas, así como llevar a cabo controles no primarios (incubando únicamente el secundario).

En cualquier caso, ambos anticuerpos están cubiertos por nuestra garantía Abpromise, y si siguieras teniendo problemas con ellos te ruego que nos contactes para ayudarte a resolverlos.

Read More

Abcam Scientific Support

回复于 Feb 29 2012

Question

Muchas gracias por tus comentarios.
Como sistema de recuperación antigénica utilizo normalmente citrato sódico y microondas.
Me comentas lo de usar suero de mismo secundario, pero en este caso no seri sheep?

Muchas gracias

Read More

Abcam community

Verified customer

Asked on Feb 01 2012

Answer

Gracias por tu respuesta.
Te confirmo que ab53435 solo se ha testado en secciones congeladas. No tenemos, pues, ninguna evidencia de que vaya a detectar el epítopo en secciones incluidas en parafina.
Así pues, puedes optar por testarlo en esta aplicación, indicándomelo previamente para que yo te envíe el código canjeable por un anticuerpo gratuito, u optar por usar ab8940.
Si eliges la última opción te aconsejo que uses el método de recuperación antigénica que te suele funcionar. Yo particularmente aconsejo Tris EDTA en microondas, optimizando el tiempo de recuperación, es decir, probando a varios tiempos, a partir de un minuto, y no más de 20, para evitar dañar la morfología del tejido.
Como agente de bloqueo, la recomendación es usar suero de la misma especie en la que se generó el secundario, para evitar uniones inespecíficas de éste. Si utilizas el Rabbit anti-Sheep IgG H&L (FITC) secondary antibody (ab6743), lo ideal sería usar suero de conejo.
Espero haberte ayudado. Recuerda que si optas por probar ab53435 en IHC-P, el código debe emitirse en primer lugar, por lo que antes de hacer el pedido te pido que me lo indiques para enviarte dicho código.
Si tienes alguna otra consulta, no dudes en volver a contactarnos.

Read More

Abcam Scientific Support

回复于 Feb 01 2012

Question

I have received ab6743 from you and have been using it as the secondary ab against a sheep anti-histone ab (Biogenesis catalog #4974-8060). I am testing N18 cells (an adherent mouse neuroblastoma cell line) to determine if histones are ever expressed on the cell surface. (Likely an artifact but the point of doing this is to confirm that it's not true). For my first attempt I used the Cytofix/Cytoperm kit by Pharmingen to permeabalize the cells. A fraction of a percent of the cells lit up. I have done this experiment2 more times using 0.4% Triton x-100 (after paraformaldehdye fixation) to permeabalize cells. None of the cells have lit up. What do you recommend for permeabilization of adherrent cells? Also, have you confirmed your ab works in this type of experiment? or with the Biogenesis ab?

Read More

Abcam community

Verified customer

Asked on Jun 12 2002

Answer

We have no experience using this secondary antibody in this technique, so I am afraid that I cannot offer any specific advice. However, this Rabbit polyclonal to sheep IgG will certainly work in conjunction with a sheep primary.

Read More

Abcam Scientific Support

回复于 Jun 12 2002

Question

I have received your antibodies, ab6743 and ab7834, and would like to know if they are stable in buffers containing 0.4% Triton X-100. I need to permeabilize cells and the protocol I intend to use calls for fixation with 4% paraformaldehyde in PBS followed by permeabilization with PBS containing 0.4% Triton X-100, 1% BSA and 4% normal serum. Will your antibodies be ok in this buffer? Thanks, Ron Knight

Read More

Abcam community

Verified customer

Asked on May 23 2002

Answer

Antibodies are usually stable in buffers containing triton-X. I've personally used antibodies in such buffers with no problems. Also, several automated IHC staining systems routinely use such surfactants in their buffer solutions. We have never actually tested ab6743 and 7834 in Triton-X containing buffers, but the odds are that they will be OK. Try a small amount for starters to make sure.

Read More

Abcam Scientific Support

回复于 May 24 2002

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

通过邮件收到学术资源和促销信息 注册
研究类别(A-Z)
  • 肿瘤研究
  • 心血管研究
  • 细胞生物学
  • 发育生物学
  • 染色质及细胞核信号转导研究
  • 免疫学
  • 代谢
  • 微生物学
  • 神经生物学
  • 信号转导研究
  • 干细胞研究
产品种类(A-Z)
  • 一抗
  • 二抗
  • 生化试剂
  • 亚类对照
  • 流式细胞仪多色选择器
  • 试剂盒
  • 内参对照
  • 细胞裂解液
  • 多肽
  • 蛋白酶
  • 组织切片
  • 蛋白标签及细胞标记物
  • 相关试剂及工具
帮助与支持
  • 技术支持
  • 技术FAQ
  • 实验步骤与建议
  • 购买FAQ
  • RabMAb 产品
  • 培训课程
  • 按靶标浏览
企业
  • 企业资讯
  • 投资者关系
  • 公司新闻
  • 招贤纳士
  • 关于我们
活动通知
  • 商品展览
  • 会议
全球网站
  • abcam.com
  • abcam.co.jp
QR code for Weechat

微信扫码 关注我们

沪ICP备14046373号-1

沪公网安备 31011502007413号

  • 销售条款
  • 网站使用条款
  • Cookie政策
  • 隐私权政策
  • 法律
© 1998-2025 艾博抗(上海)贸易有限公司版权所有