Rabbit Anti-Sheep IgG H&L (HRP)

• IHC-P

PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Sheep IgG H&L (HRP) (AB6747)

Representative immunohistochemical staining of parietal peritoneum tissue sections displaying HA in brown color (A-D). HA is dramatically reduced in the peritoneum from mice treated with PDF (B) compared to tissue from untreated mice (A) and mice pretreated with H398 2 hours before application of PDF (C) or PBS (D) (Scale bar : 100μm). The secondary antibody was Rabbit Anti-Sheep IgG H&L (HRP) (ab6747) at 1/100 dilution.

Kälble F et al. Selective Blocking of TNF Receptor 1 Attenuates Peritoneal Dialysis Fluid Induced Inflammation of the Peritoneum in Mice. PLoS One 11:e0163314 (2016).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Sheep IgG H&L (HRP) (AB6747)

ab6747 was used at dilution 1/100 with the primary antibody ab53842 in IHC-P. See the review on ab53842.

• ELISA

Supplier Data

ELISA - Rabbit Anti-Sheep IgG H&L (HRP) (AB6747)

ELISA results of ab6747 against purified Sheep IgG. Each well was coated in duplicate with 1.0 µg of Sheep IgG. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate.

• WB

Unknown

Western blot - Rabbit Anti-Sheep IgG H&L (HRP) (AB6747)

ab6747 was used at dilution 1/2000 with the primary antibody ab7832 in WB. See the review on ab7832.

Secondary

All lanes:

Western blot - Rabbit Anti-Sheep IgG H&L (HRP) (ab6747)

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• WB

Supplier Data

Western blot - Rabbit Anti-Sheep IgG H&L (HRP) (AB6747)

Western blot analysis of ab6747 used as a secondary antibody at 1/1000 dilution overnight at 2-8°C.

All lanes:

Sheep IgG

Secondary

All lanes:

Western blot - Rabbit Anti-Sheep IgG H&L (HRP) (ab6747) at 1/1000 dilution

false