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兔抗大鼠IgG H&L (Biotin) (ab6733)

Submit a review Q&A (3)References (24)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Rat IgG H&L (Biotin) (ab6733)
  • Immunohistochemistry (Frozen sections) - Rabbit Anti-Rat IgG H&L (Biotin) (ab6733)

Key features and details

  • Rabbit Anti-Rat IgG H&L (Biotin)
  • Conjugation: Biotin
  • Host species: Rabbit
  • Isotype: IgG
  • Suitable for: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Fr

Conjugates logo Related conjugates and formulations

Alkaline Phosphatase beta-galactosidase FITC HRP Unconjugated Unconjugated

概述

  • 产品名称

    兔抗大鼠IgG H&L (Biotin)
    参阅全部 IgG 二抗

  • 宿主

    Rabbit

  • 靶标种属

    Rat

  • 经测试应用

    适用于: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Frmore details

  • 免疫原

    Rat IgG whole molecule

  • 偶联物

    Biotin

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...

  • 纯度

    Affinity purified

  • 纯化说明

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

  • 共轭说明

    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab6733于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use at an assay dependent dilution.
ELISA
1/500000.
Immunomicroscopy
Use at an assay dependent concentration.
Dot blot
Use at an assay dependent concentration.
ICC/IF
1/1000 - 1/5000.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
说明
WB
Use at an assay dependent dilution.
ELISA
1/500000.
Immunomicroscopy
Use at an assay dependent concentration.
Dot blot
Use at an assay dependent concentration.
ICC/IF
1/1000 - 1/5000.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Rat IgG H&L (Biotin) (ab6733)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Rat IgG H&L (Biotin) (ab6733)This image is courtesy of an Abreview submitted by Dr Jiqiang Zhang
    Ab6640 (Rat monoclonal [CI:A3-1] to F4/80) at a 1/8000 dilution staining mouse spleen tissue by IHC-P. Ab6733 was used as the secondary antibody at a dilution of 1/400.

    See Abreview

  • Immunohistochemistry (Frozen sections) - Rabbit Anti-Rat IgG H&L (Biotin) (ab6733)
    Immunohistochemistry (Frozen sections) - Rabbit Anti-Rat IgG H&L (Biotin) (ab6733)
    Ab6733 was used at dilution 1/100 with the primary antibody ab25375 in IHC-Fr. See the review on ab25375.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (24)

发表研究结果有使用 ab6733？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab6733 被引用在 24 文献中.

  • Nakagomi E  et al. Cancer stem cell markers CD44v9+/CD133- are associated with low apoptosis in both sporadic and ulcerative colitis-associated colorectal cancers. Histol Histopathol 37:587-595 (2022). PubMed: 35224715
  • Kurhe Y  et al. Antagonizing STK25 Signaling Suppresses the Development of Hepatocellular Carcinoma Through Targeting Metabolic, Inflammatory, and Pro-Oncogenic Pathways. Cell Mol Gastroenterol Hepatol 13:405-423 (2022). PubMed: 34624527
  • Delessard M  et al. Achievement of complete in vitro spermatogenesis in testicular tissues from prepubertal mice exposed to mono- or polychemotherapy. Sci Rep 12:7407 (2022). PubMed: 35523907
  • Hall JM  et al. Mucosal Immunization with DTaP Confers Protection against Bordetella pertussis Infection and Cough in Sprague-Dawley Rats. Infect Immun 89:e0034621 (2021). PubMed: 34516235
  • Paolino M  et al. RANK links thymic regulatory T cells to fetal loss and gestational diabetes in pregnancy. Nature 589:442-447 (2021). PubMed: 33361811
View all Publications for this product

客户评价及客户问答

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1-3 of 3 Abreviews or Q&A

Question

background with ab6733 on Mouse tissue samples

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Answer

Merci de nous avoir contactés.

Nous sommes désolés d'apprendre que le produit ab6733 que vous avez reçu ne fonctionne pas comme attendu.
Comme convenu j'ai mis en place l'envoi d'une unité de ab7103 (https://www.abcam.com/ab7103), le numéro de commande de remplacement gratuit de ce produit est *****.
Vous recevrez prochainement un mail de confirmation comprenant les détails d'expédition.

Veuillez noter que cet anticorps de remplacement est également couvert par la garantie Abpromise, donc n'hésitez pas à nous contacter de nouveau si nécessaire.

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Question

Customer kindly requested Biotin conjugated secondary antibodies for ab2378, ab5690, ab103573, ab10558, ab955, ab53444, ab9018

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Answer

Thank you for your interest in Abcam products. ab2378, ab955 and ab9018 are mouse monoclonal antibodies. I would recommend using ab97044 as a secondary antibody; https://www.abcam.com/Rabbit-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-Biotin-ab97044.html ab5690, ab103573, ab10558 are rabbit polyclonal antibodies. I would recommend using ab6720; https://www.abcam.com/Rabbit-IgG-secondary-antibody-H-L-ab6720.html ab53444 is a rat monoclonal antibody. I would recommend using ab6733; https://www.abcam.com/Rat-IgG-secondary-antibody-H-L-ab6733.html To let you know we are running a promotion '4 for 3' on primaries; if you could select 1 more primary antibody then you will be entitled to 8 primary antibodies for the price of 6. Use the discount code and follow the instructions given on website. I hope this information will be helpful. Should you have any other question please do not hesitate to contact me.

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Question

Is this or any other anti-rat Ab absorbed to prevent cross reactivity with mouse Abs ? I need to run a double label withj mouse and rat Abs and I need to prevent any cross reactivity. I can't use any rabbit Abs for another technical reason. Thanks David

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Answer

I recommend ab7096 -- Goat polyclonal to rat IgG H&L (biotin) Pre-Adsorbed

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