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Immunology Immunoglobulins Heavy Chain IgG

兔抗小鼠IgG H&L (HRP) (ab6728)

  • Datasheet
  • SDS
Reviews (10)Q&A (31)References (779)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)
  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)
  • Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)
  • ELISA - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)

Key features and details

  • Rabbit Anti-Mouse IgG H&L (HRP)
  • Conjugation: HRP
  • Host species: Rabbit
  • Isotype: IgG
  • Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, ICC/IF, WB

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alkaline Phosphatase Biotin FITC Texas Red ® TRITC Unconjugated Unconjugated

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概述

  • 产品名称

    兔抗小鼠IgG H&L (HRP)
    参阅全部 IgG 二抗
  • 宿主

    Rabbit
  • 靶标种属

    Mouse
  • 经测试应用

    适用于: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, ICC/IF, WBmore details
  • 免疫原

    Mouse IgG whole molecule

  • 偶联物

    HRP

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C.
  • 存储溶液

    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • 纯度

    Affinity purified
  • 纯化说明

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads.
  • 共轭说明

    Horseradish Peroxidase (HRP)
  • 克隆

    多克隆
  • 同种型

    IgG
  • 常规说明

    Many of our customers have reported seeing brown precipitates in the vials. The brown precipitates are very common with HRP conjugated antibodies; we suggest vortexing the vial and using this antibody as normal. Our customer’s feedback says the antibody worked great. If in case the antibody fails to give results then please contact our Scientific Support team for assistance.
  • 研究领域

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Mouse
    • IgG
    • Enzyme
    • HRP

相关产品

  • Substrate reagent

    • TMB ELISA Substrate (Highest Sensitivity) (ab171522)
    • TMB ELISA Substrate (High Sensitivity) (ab171523)
    • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
    • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
    • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
    • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab6728于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Dot blot
Use at an assay dependent dilution.
ELISA
1/1000.
IHC-P
Use at an assay dependent dilution.
IHC-Fr
Use at an assay dependent dilution.
Immunomicroscopy
Use at an assay dependent dilution.
ICC/IF
1/1000 - 1/5000.
WB (10)
1/2000 - 1/10000. PubMed: 17200442
说明
Dot blot
Use at an assay dependent dilution.
ELISA
1/1000.
IHC-P
Use at an assay dependent dilution.
IHC-Fr
Use at an assay dependent dilution.
Immunomicroscopy
Use at an assay dependent dilution.
ICC/IF
1/1000 - 1/5000.
WB
1/2000 - 1/10000. PubMed: 17200442

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)Gao et al PLoS One. 2016 Apr 28;11(4):e0154419. doi: 10.1371/journal.pone.0154419. eCollection 2016. Fig2. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Distribution, expression and correlation of IL-22 cells with liver fibrosis in the liver.

    Immunohistochemistry double staining identities IL-22 cells were produced by CD4 cells in liver tissue (Panel B).

    Black arrows indicate double-positive cells.

    Paraffin-embedded, formalin-fixed human liver tissues were incubated with anti-IL-22 (ab18499) or α-SMA antibody (ab7817) overnight at 4°C followed by heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, 0.1 mol/L) for 20mins and blocking endogenous peroxidase activity with 0.3% H2O2. Revelation of primary antibody was carried out using horseradish peroxidase (HRP)-conjugated rabbit polyclonal anti-mouse (ab6728), secondary antibody followed by diamino-benzidine (DAB) and haematoxylin coloration. Double staining was performed with 3-amino-9-ethyl-carbazole (red color) for IL-22 and BCIP/ NBT (indigo color) for CD4.

    Positively stained cells were counted at high-power field (hpf, ×400) according to described protocols.

  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)
    Immunohistochemistry (PFA perfusion fixed frozen sections) - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)

    ab6728 was used at dilution 1/500 with the primary antibody ab3089 in IHC-Fr (PFA perfusion). See the review on ab3089.

  • Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)
    Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)

    ab6728 was used at dilution 1/2000 with the primary antibody ab72009 in WB. See the review on ab72009.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)This image is courtesy of an anonymous Abreview.

    ab81785 staining LC3A/B in Mouse kidney tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% protein block for 5 minutes at room temperature; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/50) for 1 hour. Anti-mouse HRP (ab6728) (1/200) was used as the secondary antibody.

  • ELISA - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)
    ELISA - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)

    ab6728 was used at dilution 1/1000 with the primary antibody ab11038 in ELISA. See the review on ab11038.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (779)

发表研究结果有使用 ab6728?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab6728 被引用在 779 文献中.

  • Hu X & Miao H MiR-539-5p inhibits the inflammatory injury in septic H9c2 cells by regulating IRAK3. Mol Biol Rep 49:121-130 (2022). PubMed: 34757596
  • Dou JY  et al. Betulin Targets Lipin1/2-Meidated P2X7 Receptor as a Therapeutic Approach to Attenuate Lipid Accumulation and Metaflammation. Biomol Ther (Seoul) 30:246-256 (2022). PubMed: 34815367
  • Sun H  et al. Long non-coding RNA H19 mediates N-acetyltransferase 1 gene methylation in the development of tamoxifen resistance in breast cancer. Exp Ther Med 23:12 (2022). PubMed: 34815764
  • Liu J  et al. Study on Toll-Like Receptor 2-Mediated Inflammation-Induced Familial Hypertension Combined with Hyperlipemia and Its Mechanism. J Healthc Eng 2022:1473597 (2022). PubMed: 35035808
  • Zhao F  et al. LINC02190 inhibits the embryo-endometrial attachment by decreasing ITGAD expression. Reproduction 163:107-118 (2022). PubMed: 35038314
View all Publications for this product

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

1-10 of 41 Abreviews or Q&A

secondary antibody ab6728

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
dilution buffer: 5% non-fat milk in PBS-Tween
dilution: 1/20000
incubation time: 1hour
exposure time: 30sec
Figure legend:
Western blot for cyclin B2 and GAPDH. HeLa Kyoto cells transfected with 50nM siRNA against HURP protein(lane 1) or 50nM non-targeting siRNA(lane 2).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Christos Efstathiou

Verified customer

提交于 Mar 09 2022

Amazing WB Secondary

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
loaded 20ug human cell lysate
reduced and denatured conditions
primary antibody was a mouse-anti-human & incubated for 16hr at 4degrees in 5% milk
secondary (ab6728) incubated for 1hr, at 1:5000 in 5% milk at 20degrees

from left to right:
Lane 1 = human cell lysate
Lane 2 = human cell lysate
Ladder
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Mar 31 2022

Works Perfect

Excellent
Abreviews
Abreviews
Application
Western blot
Works great as described.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jun 18 2021

EXCELLENT SECONDARY ANTIBODY for WB

Excellent
Abreviews
Abreviews
Application
Western blot
EXCELLENT SECONDARY ANTIBODY for WB
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MRS. Kyunga Kwak

Verified customer

提交于 May 07 2020

Very sensitive and specific secondary antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
The dilution used for this secondary antibody was 1/20000 and we obtained intesnse specific bands even at very short incubation times. It works very well with phosphorylated proteins which sometimes are harder to detect (in these cases we use 1/10000 dilution or 1/5000).

Figure legend
Western blot image of actin bands. HCT116 cells transfected with non-targeting siRNA (lane #1) or CDK4/6 siRNA (lane #2).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Oct 02 2018

Immunoblot detection of B-3-Tubulin

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
20 μg of micro tubular protein from SH-SY5y cells were used to detect B-3-Tubulin (Primary antibody - 1:500 dilution overnight, Secondary antibody (ab6728 - 1: 2000 dilution for 1h).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Feb 28 2018

Excellent 2nd antibody for WB

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
A excellent 2nd Ab for WB (1:2,000 dilution) used with ab9484 (1:10000)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Baptiste Jude

Verified customer

提交于 Oct 10 2016

This is a good 2nd antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
I used this antibody, when I perform WB.
This antibody is very sensitive.
Dilution concentration that I used is 1/2000 in TBST(5% BSA) for 1hour.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 May 30 2014

The product is good

Excellent
Abreviews
Abreviews
Application
Western blot
When I perform WB, that is very sensitive. making band is clearly
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jan 23 2014

good for mouse primary antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
strong detection
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jul 11 2013

1-10 of 41 Abreviews or Q&A

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