兔抗小鼠IgG H&L (Biotin)预吸附二抗(ab98784)
Key features and details
- Rabbit Anti-Mouse IgG H&L (Biotin) preadsorbed
- Conjugation: Biotin
- Host species: Rabbit
- Isotype: IgG
- Suitable for: IHC-P, ICC/IF, ELISA, WB
概述
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产品名称
兔抗小鼠IgG H&L (Biotin)预吸附二抗
参阅全部 IgG 二抗 -
宿主
Rabbit -
靶标种属
Mouse -
经测试应用
适用于: IHC-P, ICC/IF, ELISA, WBmore details -
最小交叉反应
Human, Rat more details -
偶联物
Biotin
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.2% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. -
克隆
多克隆 -
同种型
IgG -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab98784于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/500 - 1/5000.
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ICC/IF |
1/200 - 1/1000.
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|
ELISA |
1/20000 - 1/400000. as primary
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|
WB |
1/20000 - 1/400000. chemiluminescent detection
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说明 |
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IHC-P
1/500 - 1/5000. |
ICC/IF
1/200 - 1/1000. |
ELISA
1/20000 - 1/400000. as primary |
WB
1/20000 - 1/400000. chemiluminescent detection |
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Mouse IgG H&L (Biotin) preadsorbed (ab98784)This image is courtesy of an anonymous AbreviewImmunohistochemical analysis of dog skin tissue, detecting staining of NQO1 with ab98784.
Tissue was fixed with formaldehyde and blocked with none for ; antigen retrieval was by heat mediation in a citrate buffer (pH 6). Samples were incubated with primary antibody (ab28947 Mouse monoclonal [A180] to NQO1 at 1/175 in BSA in TBS) for 45 minutes. ab98784 (1/500) was used as the secondary antibody.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
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