兔抗小鼠IgG H&L (Biotin)预吸附二抗(ab7074)
Key features and details
- Rabbit Anti-Mouse IgG H&L (Biotin) preadsorbed
- Conjugation: Biotin
- Host species: Rabbit
- Suitable for: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB
概述
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产品名称
兔抗小鼠IgG H&L (Biotin)预吸附二抗
参阅全部 IgG 二抗 -
宿主
Rabbit -
靶标种属
Mouse -
经测试应用
适用于: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details -
最小交叉反应
Human more details -
免疫原
Full length native Mouse IgG (purified).
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偶联物
Biotin
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading... -
纯度
Affinity purified -
纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
共轭说明
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule -
克隆
多克隆 -
研究领域
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | ||
| Dot blot | ||
| ELISA | ||
| IHC-P | ||
| IHC-Fr | ||
| Immunomicroscopy | ||
| WB |
| 说明 |
|---|
应用说明
Dot: Use at an assay dependant dilution.
ELISA: 1/20000 - 1/80000.
IHC-P: Use at an assay dependant dilution.
IHC-Fr: Use at an assay dependant dilution.
IM: Use at an assay dependant dilution.
WB: Use at an assay dependant dilution.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ELISA: 1/20000 - 1/80000.
IHC-P: Use at an assay dependant dilution.
IHC-Fr: Use at an assay dependant dilution.
IM: Use at an assay dependant dilution.
WB: Use at an assay dependant dilution.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (10)
ab7074 被引用在 10 文献中.
- Badr El-Din NK et al. Thymax, a gross thymic extract, exerts cell cycle arrest and apoptosis in Ehrlich ascites carcinoma in vivo. Heliyon 8:e09047 (2022). PubMed: 35299600
- Luan N et al. TRF-20-M0NK5Y93 suppresses the metastasis of colon cancer cells by impairing the epithelial-to-mesenchymal transition through targeting Claudin-1. Am J Transl Res 13:124-142 (2021). PubMed: 33527013
- Bryniarski MA et al. Megalin-mediated albumin endocytosis in cultured murine mesangial cells. Biochem Biophys Res Commun 529:740-746 (2020). PubMed: 32736701
- Binch A et al. Immunohistochemical analysis of protein expression in formalin fixed paraffin embedded human intervertebral disc tissues. JOR Spine 3:e1098 (2020). PubMed: 33015573
- Rustenburg CM et al. Modelling the catabolic environment of the moderately degenerated disc with a caprine ex vivo loaded disc culture system. Eur Cell Mater 40:21-37 (2020). PubMed: 32672345
- Ou Y et al. Different MAPK signal transduction pathways play different roles in the impairment of glucose-stimulated insulin secretion in response to IL-1ß. Mol Med Rep 22:2973-2980 (2020). PubMed: 32945406
- Zheng C et al. Effect of ATM on inflammatory response and autophagy in renal tubular epithelial cells in LPS-induced septic AKI. Exp Ther Med 18:4707-4717 (2019). PubMed: 31777559
- Snuggs JW et al. Aquaporin expression in the human and canine intervertebral disc during maturation and degeneration. JOR Spine 2:e1049 (2019). PubMed: 31463463
- Rughoobur G et al. Gravimetric sensors operating at 1.1?GHz based on inclined c-axis ZnO grown on textured Al electrodes. Sci Rep 7:1367 (2017). PubMed: 28465522
- Grondin Y et al. Genetic Polymorphisms Associated with Hearing Threshold Shift in Subjects during First Encounter with Occupational Impulse Noise. PLoS One 10:e0130827 (2015). PubMed: 26121033
