兔抗小鼠IgG H&L (ab6709)
Key features and details
- Rabbit Anti-Mouse IgG H&L
- Host species: Rabbit
- Suitable for: WB, IP, Immunodiffusion, Conjugation, ICC/IF, ELISA, IHC-P, IHC-Fr
Related conjugates and formulations
概述
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产品名称
兔抗小鼠IgG H&L
参阅全部 IgG 二抗 -
宿主
Rabbit -
靶标种属
Mouse -
经测试应用
适用于: WB, IP, Immunodiffusion, Conjugation, ICC/IF, ELISA, IHC-P, IHC-Frmore details -
免疫原
Mouse IgG, whole molecule
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
纯度
Affinity purified -
纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads -
克隆
多克隆 -
研究领域
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000.
|
|
| IP |
Use at an assay dependent concentration.
|
|
| Immunodiffusion |
Use at an assay dependent concentration.
|
|
| Conjugation |
Use at an assay dependent concentration.
|
|
| ICC/IF |
1/1000 - 1/5000.
|
|
| ELISA |
1/20000 - 1/400000.
|
|
| IHC-P |
Use at an assay dependent concentration.
|
|
| IHC-Fr |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
WB
1/2000 - 1/10000. |
|
IP
Use at an assay dependent concentration. |
|
Immunodiffusion
Use at an assay dependent concentration. |
|
Conjugation
Use at an assay dependent concentration. |
|
ICC/IF
1/1000 - 1/5000. |
|
ELISA
1/20000 - 1/400000. |
|
IHC-P
Use at an assay dependent concentration. |
|
IHC-Fr
Use at an assay dependent concentration. |
图片
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ELISA - Rabbit Anti-Mouse IgG H&L (ab6709)
Sensitivity of ab6709 against mouse IgG.
The starting dilution of ab6709 was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. The blocking agent was 3% fish gelatin. The detection antibody was a Goat anti-Rabbit IgG Antibody Peroxidase conjugate. TMB color development.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (45)
ab6709 被引用在 45 文献中.
- Garcia-Martin R et al. MicroRNA sequence codes for small extracellular vesicle release and cellular retention. Nature 601:446-451 (2022). PubMed: 34937935
- Weiss JM et al. Anatomic position determines oncogenic specificity in melanoma. Nature 604:354-361 (2022). PubMed: 35355015
- Ruan Z et al. Functional genome-wide short hairpin RNA library screening identifies key molecules for extracellular vesicle secretion from microglia. Cell Rep 39:110791 (2022). PubMed: 35545052
- Kraft K et al. Polycomb-mediated genome architecture enables long-range spreading of H3K27 methylation. Proc Natl Acad Sci U S A 119:e2201883119 (2022). PubMed: 35617427
- Kitamura K et al. Dual function of SF3B2 on chromatin and RNA to regulate transcription in head and neck squamous cell carcinoma. Cell Biosci 12:92 (2022). PubMed: 35715826