重组兔单克隆抗体[RM118]抗人IgG2 heavy chain (Biotin) (ab272184)
Key features and details
- Rabbit monoclonal [RM118] Anti-Human IgG2 heavy chain (Biotin)
- Conjugation: Biotin
- Host species: Rabbit
- Isotype: IgG
- Suitable for: ELISA
概述
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产品名称
兔单克隆抗体[RM118]抗人IgG2 heavy chain (Biotin) -
宿主
Rabbit -
靶标种属
Human -
特异性
ab272184 reacts to the heavy chain of human IgG2. No cross reactivity with human IgG1, IgG3, IgG4, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.
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经测试应用
适用于: ELISAmore details -
免疫原
Full length protein corresponding to Human IgG2.
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偶联物
Biotin
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Store In the Dark. -
存储溶液
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
纯度
Protein A purified -
纯化说明
From an animal origin–free culture supernatant. -
克隆
单克隆 -
克隆编号
RM118 -
同种型
IgG -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272184于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
Capture: 50ng/well – 200ng/well |
| 说明 |
|---|
|
ELISA
Use at an assay dependent concentration. Capture: 50ng/well – 200ng/well |
图片
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ELISA - Rabbit monoclonal RM118 Anti-Human IgG heavy chain (Biotin) (ab272184)
A titer ELISA using ab272184. The plate was coated with different amounts of human IgG2. A serial dilution of ab272184 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG was used as the secondary antibody.
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ELISA - Rabbit monoclonal RM118 Anti-Human IgG heavy chain (Biotin) (ab272184)ELISA of human immunoglobulins shows ab272184 only reacted to human IgG2. No cross reactivity with Human IgG1, IgG3, IgG4, IgE, IgD, IgA, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. A serial dilution of ab272184 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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Sandwich ELISA - Rabbit monoclonal RM118 Anti-Human IgG heavy chain (Biotin) (ab272184)Sandwich ELISA using ab272184 as the capture antibody, and a Biotinylated anti-human light chains (κ+ λ) antibody ab193185 as the detection antibody, followed by an AP conjugated streptavidin.
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Sandwich ELISA - Rabbit monoclonal RM118 Anti-Human IgG heavy chain (Biotin) (ab272184)Sandwich ELISA using ab272184 as the capture antibody, and a Biotinylated anti-human light chains (κ+ λ) antibody ab193185 as the detection antibody, followed by an AP conjugated streptavidin.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab272184 尚未被引用在任何文献中。