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兔抗人IgG H&L (HRP) (ab6759)

Reviews (1) Submit a question References (95)
ELISA - Rabbit Anti-Human IgG H&L (HRP) (ab6759)

    Key features and details

    • Rabbit Anti-Human IgG H&L (HRP)
    • Conjugation: HRP
    • Host species: Rabbit
    • Isotype: IgG
    • Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB, ICC/IF, Competitive ELISA

    Conjugates logo Related conjugates and formulations

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    概述

    • 产品名称

      兔抗人IgG H&L (HRP)
      参阅全部 IgG 二抗

    • 宿主

      Rabbit

    • 靶标种属

      Human

    • 经测试应用

      适用于: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB, ICC/IF, Competitive ELISAmore details

    • 免疫原

      human IgG whole molecule

    • 偶联物

      HRP

    性能

    • 形式

      Liquid

    • 存放说明

      Shipped at 4°C. Store at +4°C.

    • 存储溶液

      Preservative: 0.01% Gentamicin sulphate
      Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
    • Concentration information loading...

    • 纯度

      Affinity purified

    • 纯化说明

      This product was prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

    • 共轭说明

      Horseradish Peroxidase (HRP)

    • 克隆

      多克隆

    • 同种型

      IgG

    • 研究领域

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab6759于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    Dot blot
    Use at an assay dependent dilution.
    ELISA (1)
    1/130000.
    IHC-P
    Use at an assay dependent dilution.
    IHC-Fr
    Use at an assay dependent dilution.
    Immunomicroscopy
    Use at an assay dependent dilution.
    WB
    1/5000 - 1/25000.
    ICC/IF
    1/1000 - 1/5000.
    Competitive ELISA
    Use at an assay dependent dilution. PubMed: 20573830
    说明
    Dot blot
    Use at an assay dependent dilution.
    ELISA
    1/130000.
    IHC-P
    Use at an assay dependent dilution.
    IHC-Fr
    Use at an assay dependent dilution.
    Immunomicroscopy
    Use at an assay dependent dilution.
    WB
    1/5000 - 1/25000.
    ICC/IF
    1/1000 - 1/5000.
    Competitive ELISA
    Use at an assay dependent dilution. PubMed: 20573830

    图片

    • ELISA - Rabbit Anti-Human IgG H&L (HRP) (ab6759)
      ELISA - Rabbit Anti-Human IgG H&L (HRP) (ab6759)

      ELISA results of ab6759 tested against purified human IgG protein.

      Each well was coated in duplicate with 1.0 µg of human IgG. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log 10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate.

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (95)

    发表研究结果有使用 ab6759？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab6759 被引用在 95 文献中.

    • Zhang X  et al. Altered genome‑wide hydroxymethylation analysis for neoadjuvant chemoradiotherapy followed by surgery in esophageal cancer. Exp Ther Med 25:29 (2023). PubMed: 36561617
    • Abu-Humaidan AHA  et al. Anti-SARS-Cov-2 S-RBD IgG Formed after BNT162b2 Vaccination Can Bind C1q and Activate Complement. J Immunol Res 2022:7263740 (2022). PubMed: 36573216
    • Ke W  et al. miR-134-5p promotes inflammation and apoptosis of trophoblast cells via regulating FOXP2 transcription in gestational diabetes mellitus. Bioengineered 13:319-330 (2022). PubMed: 34969354
    • Li Y  et al. Macrophages activated by hepatitis B virus have distinct metabolic profiles and suppress the virus via IL-1β to downregulate PPARα and FOXO3. Cell Rep 38:110284 (2022). PubMed: 35081341
    • Yu Y & Zhu T RAR-Related Orphan Receptor: An Accelerated Preeclampsia Progression by Activating the JAK/STAT3 Pathway. Yonsei Med J 63:554-563 (2022). PubMed: 35619579
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    Intracellular Alpha-galactosidase A/antibody-complex detection from enzyme-treated EA.hy926 cells

     Excellent
    Abreviews
    abreview image
    Application
    ELISA
    Cells were seeded on 96-well plates with a density of 2x105 cell/ml and grown until confluent. After 4 hours of incubation with human recombinant α-galactosidase A (AGAL, 4.6 μg/ml) and patients’ serum (50 µl per 4.6 μg/ml AGAL), cells were washed twice with PBS and lysed with passive lysis buffer (Promega, Mannheim Germany). Lysates were centrifuged (14.000x g) for 20 minutes at 4°C and supernatants were used for ELISAs, as follows. 96 well plates were coated with a capture antibody (α-AGAL, R&D Sytems, Minneapolis, US no. AF6146, 200 ng per well) over night at 4°C. After 5 washes with 200 μl PBS/Tween 0.1% and blocking with PBS/BSA 2% for 2 hours at RT, 3-10 μl of cellular lysates were applied in 100 μl PBS overnight at 4°C. Wells were washed 3 times with PBS/Tween 0.1%. For human IgG detection shuttled into cells by AGAL, anti-hIgG HRP-coupled antibodies (Abcam no: ab6759, working concentration: 0.66 μg/ml) were applied in PBS/BSA 2% for 2 hours at RT. After 5 additional washes, 100 μl 1-Step Ultra TMB-ELISA Substrate Solution (Thermo Fisher Scientific) was added to each well, incubated at RT, and stopped with 100 μl 2 mol/l sulfuric acid. Absorption was measured at 450 nm and normalized for protein content and against serum from a healthy control (negativ for antibodies against AGAL).

    Figure legend: *p<0.05, **p<0.01.
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    提交于 Sep 04 2019

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