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兔抗山羊IgG H&L (HRP)预吸附二抗(ab7132)

Submit a review Q&A (4)References (6)

Key features and details

  • Rabbit Anti-Goat IgG H&L (HRP) preadsorbed
  • Conjugation: HRP
  • Host species: Rabbit
  • Suitable for: ELISA, IHC-P, IHC-Fr, WB, ICC

概述

  • 产品名称

    兔抗山羊IgG H&L (HRP)预吸附二抗
    参阅全部 IgG 二抗

  • 宿主

    Rabbit

  • 靶标种属

    Goat

  • 特异性

    No reaction was observed against Human Serum Proteins.

  • 经测试应用

    适用于: ELISA, IHC-P, IHC-Fr, WB, ICCmore details

  • 最小交叉反应


    Human more details

  • 免疫原

    Goat IgG whole molecule

  • 偶联物

    HRP

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C.

  • 存储溶液

    Preservative: 0.01% Gentamicin sulphate
    Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride
  • Concentration information loading...

  • 纯度

    Affinity purified

  • 纯化说明

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

  • 共轭说明

    Horseradish Peroxidase (HRP)

  • 克隆

    多克隆

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab7132于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ELISA
1/20000 - 1/120000.
IHC-P
1/500 - 1/2500.
IHC-Fr
1/500 - 1/2500.
WB
1/1000 - 1/5000.
ICC
1/500 - 1/2500.
说明
ELISA
1/20000 - 1/120000.
IHC-P
1/500 - 1/2500.
IHC-Fr
1/500 - 1/2500.
WB
1/1000 - 1/5000.
ICC
1/500 - 1/2500.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (6)

发表研究结果有使用 ab7132？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab7132 被引用在 6 文献中.

  • Moberg M  et al. High-intensity leg cycling alters the molecular response to resistance exercise in the arm muscles. Sci Rep 11:6453 (2021). PubMed: 33742064
  • Issa AR  et al. The lysosomal membrane protein LAMP2A promotes autophagic flux and prevents SNCA-induced Parkinson disease-like symptoms in the Drosophila brain. Autophagy 14:1898-1910 (2018). PubMed: 29989488
  • Rundqvist HC  et al. Influence of nutrient ingestion on amino acid transporters and protein synthesis in human skeletal muscle after sprint exercise. J Appl Physiol (1985) 123:1501-1515 (2017). PubMed: 28860165
  • Moberg M  et al. Activation of mTORC1 by leucine is potentiated by branched-chain amino acids and even more so by essential amino acids following resistance exercise. Am J Physiol Cell Physiol 310:C874-84 (2016). PubMed: 27053525
  • Apró W  et al. Resistance exercise-induced S6K1 kinase activity is not inhibited in human skeletal muscle despite prior activation of AMPK by high-intensity interval cycling. Am J Physiol Endocrinol Metab 308:E470-81 (2015). PubMed: 25605643
  • Kassner N  et al. CBR1 is a predominant doxorubicin reductase in the human liver. Drug Metab Dispos : (2008). WB . PubMed: 18635746

客户评价及客户问答

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1-4 of 4 Abreviews or Q&A

Question

What is best secondary antibody you can offer for ab10256.

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Answer

Thank you for contacting us.

Please check the datasheet and selct on basis of conc, price and volume. The compatible products are ab102345, ab102354, ab5755, ab97100, ab97105 and ab6741.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question

Dear Sir/Madam,



I am trying to order an antibody from your website but i can’t seem to find the product anywhere on-line, perhaps it has been discontinued or is out of stock?



The product # is 92281 a MAFbx primary antibody. I also needed the appropriate secondary goat antibody (# 7132) which is also unavailable. Can you suggest the next best possible alternatives please? Or let me know if these antibodies are likely to be in stock sometime soon?



Many thanks

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Answer

Thank you for your enquiry and your interest in our products.



I can confirm that ab92281 is Anti-Fbx32 antibody and ab7132 is Rabbit anti-Goat IgG H&L (HRP) secondary antibody; and both products are still published and available.



ab92281: https://www.abcam.com/fbx32-antibody-ab92281.html

ab7132: https://www.abcam.com/rabbit-polyclonal-secondary-antibody-to-goat-igg-h-l-hrp-pre-adsorbed-ab7132.html



If you need any further assistance in the future, please do not hesitate to contact me.

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Question

Hi,   What secondary HRP-linked antibody would you recommend after incubation with ab92281 (FBX32 ab)?

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Answer

Thank you for your enquiry. I can recommend the following HRP-conjugated secondary antibody based on your requirements: Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (HRP), pre-adsorbed (ab7132) I hope this is helpful. Please contact us again if you have any further questions.

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Question

I have sheep polyclonal to 5-methyl cytosine antibody (ab1884) as first antibody, and Rabbit polyclonal to goat IgG H&L (HRP) (ab7132) as second antibody. Dose rabbit IgG to goat react with sheep IgG?

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Answer

Thank you for your enquiry. I have been in touch with the lab and determined that this antibody has NOT been preabsorbed against sheep IgG. I am sorry that I cannot be of more assistance on this occasion.

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