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Immunology Immunoglobulins Heavy Chain IgG

兔抗山羊IgG H&L (Biotin)预吸附二抗(ab7131)

  • Datasheet
  • SDS
Submit a review Q&A (2)References (1)

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Abpromise

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Key features and details

  • Rabbit Anti-Goat IgG H&L (Biotin) preadsorbed
  • Conjugation: Biotin
  • Host species: Rabbit
  • Suitable for: ICC/IF, Dot blot, ELISA, IHC-P, Immunomicroscopy, WB, Flow Cyt, IHC-Fr

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概述

  • 产品名称

    兔抗山羊IgG H&L (Biotin)预吸附二抗
    参阅全部 IgG 二抗
  • 宿主

    Rabbit
  • 靶标种属

    Goat
  • 特异性

    No reaction was observed against Human Serum Proteins.

  • 经测试应用

    适用于: ICC/IF, Dot blot, ELISA, IHC-P, Immunomicroscopy, WB, Flow Cyt, IHC-Frmore details
  • 最小交叉反应


    Human
    To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
    more details
  • 免疫原

    Goat IgG whole molecule

  • 偶联物

    Biotin

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.01% Sodium azide
    Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride
  • Concentration information loading...
  • 纯度

    Affinity purified
  • 纯化说明

    Anti-Goat IgG (H&L) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • 共轭说明

    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule
  • 克隆

    多克隆
  • 研究领域

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Goat
    • IgG
    • Biotin

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab7131于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
1/1000 - 1/5000.
Dot blot
Use at an assay dependent concentration.
ELISA
Use at an assay dependent dilution.
IHC-P
Use at an assay dependent concentration.
Immunomicroscopy
Use at an assay dependent concentration.
WB
Use at an assay dependent dilution.
Flow Cyt
Use at an assay dependent dilution. PubMed: 20838433
IHC-Fr
Use at an assay dependent concentration.
说明
ICC/IF
1/1000 - 1/5000.
Dot blot
Use at an assay dependent concentration.
ELISA
Use at an assay dependent dilution.
IHC-P
Use at an assay dependent concentration.
Immunomicroscopy
Use at an assay dependent concentration.
WB
Use at an assay dependent dilution.
Flow Cyt
Use at an assay dependent dilution. PubMed: 20838433
IHC-Fr
Use at an assay dependent concentration.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (1)

发表研究结果有使用 ab7131?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab7131 被引用在 1 文献中.

  • Fernandez P  et al. Strain-transcendent immune response to recombinant Var2CSA DBL5-e domain block P. falciparum adhesion to placenta-derived BeWo cells under flow conditions. PLoS One 5:e12558 (2010). Flow Cyt . PubMed: 20838433

客户评价及客户问答

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1-2 of 2 Abreviews or Q&A

Question

What information do you require regarding the 3 antibodies? Due to the research we are doing, we don't want to name what the antibodies are raised to, but I can give the following information:

Ab1: mouse monoclonal IgG2A, stored in PBS (pH 7.4) and Protein A and G purified

Ab2: mouse monoclonal IgG1, stored in PBS (pH 7.4) and Protein G purified

Ab3: mouse monoclonal IgG1K, stored in PBS (pH 7.4) and Protein G purified

Is there any other information you require on the coating antibodies?

Best Wishes,

Read More

Abcam community

Verified customer

Asked on Apr 25 2012

Answer

Thank you for your message and for providing this further information.

Reviewing the details, I agree there seems to be some crossreactivity to mouse IgG. I can confirm that this antibody has not specifically been pre absorbedwith mouse, so we cannot guarantee it would not crossreact with mouse. However, I appreciate the time you have spent on these experiments and would be pleased to arrange afree of charge replacementor credit note in compensation.

If you decide on a free of charge replacement, I can suggest it would be beneficial to select an alternativeantibody that has been cross absorbed with mouse, so there is minimal possible crossreactivity. For example you may like to consider the following which has been tested and guaranteed in ELISA:

ab7124 Donkey anti-Goat IgG H&L (Biotin) secondary antibody
https://www.abcam.com/Donkey-Goat-IgG-HL-Biotin-preadsorbed-ab7124.html (or use the following: https://www.abcam.com/Donkey-Goat-IgG-HL-Biotin-preadsorbed-ab7124.html).

I look forward to hearing from you with details of how you would like to proceed.

Read More

Abcam Scientific Support

回复于 Apr 25 2012

Question

We are using ab7131 secondary antibody and it is showing signs of crossreactivity to mouse Ig in ELISA.

Read More

Abcam community

Verified customer

Asked on Apr 20 2012

Answer

Thank you for your telephone call yesterday afternoon. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody and have some concerns about crossreactivity to mouse..

I would like to reassure you that this antibody is tested and covered by our 6 month guarantee. In the event that a product is not functioning in the tested applications and species cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

As discussed on the telephone, I would like to investigate this particular case further for you, and also obtain more information for our quality monitoring records. In order to proceed with this, I have enclosed a technical questionnaire below. I would appreciate if you could complete this. It will help you put the information we require together very easily.

I would appreciate if you could also provide any data which would help us to assess the results.

Thank you for your time and cooperation. We look forward to receiving the completed questionaire.



Order Details
Antibody code:

Problem
Choose: Problem with standard curve No signal or weak signal High background


Lot number


Purchase order number
or preferably Abcam order number


General Information
Antibody storage conditions (temperature/reconstitution etc)


Description of the problem (high background, no signal, non-specific color development, poor standard curve etc.)


Type of ELISA (Direct ELISA/Indirect ELISA/Sandwich ELISA etc.)


Sample (Species/Cell type/Cell line etc.)


Coating well (Buffer/Concentration of the coating material etc.)


Blocking conditions (Buffer/time period, Blocking agent etc.)


Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


Detection method (Substrate/Diluent etc.)


Positive and negative controls used (please specify)




Optimization attempts (problem solving)
How many times have you tried the ELISA?



Have you run a "No Primary" control?


Yes No
Do you obtain the same results every time?
Yes No

What steps have you altered?


Additional Notes:



Data:
We would appreciate if you are able to provide a copy of the data, particularly from the standard curve. This will help us to assess the results.

Read More

Abcam Scientific Support

回复于 Apr 20 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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