Rabbit Anti-Goat IgG H&L (Alkaline Phosphatase)

• ELISA

Unknown

ELISA - Rabbit Anti-Goat IgG H&L (Alkaline Phosphatase) (AB6742)

ab6742 was used at dilution 1/4000 with the primary antibody ab20773 in ELISA. See the review on ab20773.

• ELISA

Supplier Data

ELISA - Rabbit Anti-Goat IgG H&L (Alkaline Phosphatase) (AB6742)

ELISA results of ab6742 against purified Goat IgG. (Green Line). Each well was coated in duplicate with 1.0 µg of antigen. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB substrate.

• WB

Supplier Data

Western blot - Rabbit Anti-Goat IgG H&L (Alkaline Phosphatase) (AB6742)

Western blot analysis of ab6742 used as a secondary antibody at 1/1000 dilution for 60 min at RT.

All lanes:

Goat IgG at 100 ng

Secondary

All lanes:

Western blot - Rabbit Anti-Goat IgG H&L (Alkaline Phosphatase) (ab6742) at 1/1000 dilution

false

• Dot

Supplier Data

Dot Blot - Rabbit Anti-Goat IgG H&L (Alkaline Phosphatase) (AB6742)

Dot blot analysis of ab6742. Detected using Alkaline Phosphatase Substrate (ab7468).

All lanes:

Dot Blot - Rabbit Anti-Goat IgG H&L (Alkaline Phosphatase) (ab6742)

Lane 1:

Goat IgG at 200 ng

Lane 2:

Goat IgG at 66.67 ng

Lane 3:

Goat IgG at 22.2 ng

Lane 4:

Goat IgG at 7.4 ng

Lane 5:

Goat IgG at 2.47 ng

Secondary

All lanes:

Goat IgG (H&L) Secondary Antibody Alkaline Phosphatase Conjugated at 1/1000 dilution

false