兔F(ab')2抗小鼠IgG - H&L (PE),预吸附二抗(ab7000)
Key features and details
- Rabbit F(ab')2 Anti-Mouse IgG - H&L (PE), pre-adsorbed
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Host species: Rabbit
- Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr
Related conjugates and formulations
概述
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产品名称
兔F(ab')2抗小鼠IgG - H&L (PE),预吸附二抗
参阅全部 IgG 二抗 -
宿主
Rabbit -
靶标种属
Mouse -
特异性
No reaction was observed against anti-Pepsin, anti-Rabbit IgG F(c) or Human Serum Proteins.
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经测试应用
适用于: ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Frmore details -
最小交叉反应
Human more details -
免疫原
Full length native Mouse IgG (purified).
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偶联物
PE. Ex: 488nm, Em: 575nm
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.42% Tripotassium orthophosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
共轭说明
R-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD566nm/OD280nm: 2.8 -
克隆
多克隆 -
常规说明
Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
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研究领域
应用
应用 | Ab评论 | 说明 |
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ICC/IF |
1/100 - 1/250.
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Immunomicroscopy |
1/50 - 1/200.
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|
Flow Cyt |
1/100 - 1/250. Use 1 x 106 cells in flow cytometry is approximately 1.0 mg of antibody conjugate.
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IHC-P |
1/50 - 1/200.
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IHC-Fr |
1/50 - 1/200.
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说明 |
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ICC/IF
1/100 - 1/250. |
Immunomicroscopy
1/50 - 1/200. |
Flow Cyt
1/100 - 1/250. Use 1 x 106 cells in flow cytometry is approximately 1.0 mg of antibody conjugate. |
IHC-P
1/50 - 1/200. |
IHC-Fr
1/50 - 1/200. |
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab7000 被引用在 5 文献中.
- Tian L et al. IRGM promotes melanoma cell survival through autophagy and is a promising prognostic biomarker for clinical application. Mol Ther Oncolytics 20:187-198 (2021). PubMed: 33665357
- Shi C et al. NEAT1 promotes the repair of abdominal aortic aneurysms of endothelial progenitor cells via regulating miR-204-5p/Ang-1. Am J Transl Res 13:2111-2126 (2021). PubMed: 34017378
- Amjadi F et al. Distinct changes in the proteome profile of endometrial tissues in polycystic ovary syndrome compared with healthy fertile women. Reprod Biomed Online N/A:N/A (2018). WB, IHC-P . PubMed: 29729850
- Lin Y et al. Isoliquiritigenin inhibits the proliferation and induces the differentiation of human glioma stem cells. Oncol Rep 39:687-694 (2018). PubMed: 29251326
- Liu XL et al. Differentiation of genetically modified canine bone mesenchymal stem cells labeled with superparamagnetic iron oxide into neural-like cells. Mol Med Rep 17:7902-7910 (2018). PubMed: 29620288