兔F(ab')2抗山羊IgG - H&L (HRP),预吸附二抗(ab5755)
Key features and details
- Rabbit F(ab')2 Anti-Goat IgG - H&L (HRP), pre-adsorbed
- Conjugation: HRP
- Host species: Rabbit
- Isotype: IgG
- Suitable for: ICC/IF, Dot blot, WB, ELISA, Electron Microscopy, IHC
概述
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产品名称
兔F(ab')2抗山羊IgG - H&L (HRP),预吸附二抗
参阅全部 IgG 二抗 -
宿主
Rabbit -
靶标种属
Goat -
特异性
No reaction was observed against anti-Pepsin, anti-Rabbit IgG F(c) or Human Serum Proteins.
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经测试应用
适用于: ICC/IF, Dot blot, WB, ELISA, Electron Microscopy, IHCmore details -
最小交叉反应
Human more details -
免疫原
Goat IgG whole molecule.
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偶联物
HRP
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 6.50
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.424% Tripotassium orthophosphate, 0.88% Sodium chloride, 1% BSA
BSA is IgG and protease free -
Concentration information loading... -
纯度
Affinity purified -
纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
共轭说明
Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons) -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/500 - 1/2500.
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| Dot blot |
Use at an assay dependent concentration.
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| WB |
1/1000 - 1/5000.
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| ELISA |
1/10000 - 1/50000. This product has been assayed against 1.0 ug of Goat IgG in a standard capture ELISA using ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
|
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| Electron Microscopy |
Use at an assay dependent concentration.
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| IHC |
Use at an assay dependent concentration.
|
| 说明 |
|---|
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ICC/IF
1/500 - 1/2500. |
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Dot blot
Use at an assay dependent concentration. |
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WB
1/1000 - 1/5000. |
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ELISA
1/10000 - 1/50000. This product has been assayed against 1.0 ug of Goat IgG in a standard capture ELISA using ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. |
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Electron Microscopy
Use at an assay dependent concentration. |
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IHC
Use at an assay dependent concentration. |
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (3)
ab5755 被引用在 3 文献中.
- Li Q et al. Poricoic acid A suppresses TGF-ß1-induced renal fibrosis and proliferation via the PDGF-C, Smad3 and MAPK pathways. Exp Ther Med 21:289 (2021). PubMed: 33717232
- Díaz-Jiménez D et al. A functional IL1RL1 variant regulates corticosteroid-induced sST2 expression in ulcerative colitis. Sci Rep 7:10180 (2017). PubMed: 28860510
- Belian E et al. Forward Programming of Cardiac Stem Cells by Homogeneous Transduction with MYOCD plus TBX5. PLoS One 10:e0125384 (2015). WB . PubMed: 26047103
