兔F(ab')2抗山羊IgG - H&L (Biotin),预吸附二抗(ab5753)
Key features and details
- Rabbit F(ab')2 Anti-Goat IgG - H&L (Biotin), pre-adsorbed
- Conjugation: Biotin
- Host species: Rabbit
- Isotype: IgG
- Suitable for: WB, ICC/IF, Dot blot, ELISA, IHC, Immunomicroscopy, Flow Cyt
概述
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产品名称
兔F(ab')2抗山羊IgG - H&L (Biotin),预吸附二抗
参阅全部 IgG 二抗 -
宿主
Rabbit -
靶标种属
Goat -
特异性
No reaction was observed against Human Serum Proteins.
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经测试应用
适用于: WB, ICC/IF, Dot blot, ELISA, IHC, Immunomicroscopy, Flow Cytmore details -
最小交叉反应
Human more details -
免疫原
Goat IgG whole molecule
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偶联物
Biotin
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
BSA is IgG and protease free -
Concentration information loading... -
纯度
Affinity purified -
纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
共轭说明
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule -
克隆
多克隆 -
同种型
IgG -
研究领域
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000.
|
|
| ICC/IF |
1/1000 - 1/5000.
|
|
| Dot blot |
1/10000.
|
|
| ELISA |
1/20000 - 1/100000.
|
|
| IHC |
1/500 - 1/5000.
|
|
| Immunomicroscopy |
Use at an assay dependent dilution.
|
|
| Flow Cyt |
1/200 - 1/1000.
|
| 说明 |
|---|
|
WB
1/2000 - 1/10000. |
|
ICC/IF
1/1000 - 1/5000. |
|
Dot blot
1/10000. |
|
ELISA
1/20000 - 1/100000. |
|
IHC
1/500 - 1/5000. |
|
Immunomicroscopy
Use at an assay dependent dilution. |
|
Flow Cyt
1/200 - 1/1000. |
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (1)
ab5753 被引用在 1 文献中.
- Chamberlain G et al. Murine mesenchymal stem cells exhibit a restricted repertoire of functional chemokine receptors: comparison with human. PLoS ONE 3:e2934 (2008). Flow Cyt . PubMed: 18698345
