兔抗小鼠IgG H&L (ab6709)
Key features and details
- Rabbit Anti-Mouse IgG H&L
- Host species: Rabbit
- Suitable for: WB, IP, Immunodiffusion, Conjugation, ICC/IF, ELISA, IHC-P, IHC-Fr
Related conjugates and formulations
概述
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产品名称
兔抗小鼠IgG H&L
参阅全部 IgG 二抗 -
宿主
Rabbit -
靶标种属
Mouse -
经测试应用
适用于: WB, IP, Immunodiffusion, Conjugation, ICC/IF, ELISA, IHC-P, IHC-Frmore details -
免疫原
Mouse IgG, whole molecule
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading...
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纯度
Affinity purified -
纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads -
克隆
多克隆 -
研究领域
应用
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/2000 - 1/10000.
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|
IP |
Use at an assay dependent concentration.
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|
Immunodiffusion |
Use at an assay dependent concentration.
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|
Conjugation |
Use at an assay dependent concentration.
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|
ICC/IF |
1/1000 - 1/5000.
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|
ELISA |
1/20000 - 1/400000.
|
|
IHC-P |
Use at an assay dependent concentration.
|
|
IHC-Fr |
Use at an assay dependent concentration.
|
说明 |
---|
WB
1/2000 - 1/10000. |
IP
Use at an assay dependent concentration. |
Immunodiffusion
Use at an assay dependent concentration. |
Conjugation
Use at an assay dependent concentration. |
ICC/IF
1/1000 - 1/5000. |
ELISA
1/20000 - 1/400000. |
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
图片
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Sensitivity of ab6709 against mouse IgG.
The starting dilution of ab6709 was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. The blocking agent was 3% fish gelatin. The detection antibody was a Goat anti-Rabbit IgG Antibody Peroxidase conjugate. TMB color development.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (50)
ab6709 被引用在 50 文献中.
- Read KA et al. Aiolos represses CD4+ T cell cytotoxic programming via reciprocal regulation of TFH transcription factors and IL-2 sensitivity. Nat Commun 14:1652 (2023). PubMed: 36964178
- Qiu P et al. SPI1 Mediates N-Myristoyltransferase 1 to Advance Gastric Cancer Progression via PI3K/AKT/mTOR Pathway. Can J Gastroenterol Hepatol 2023:2021515 (2023). PubMed: 36967718
- Wang K et al. Intercellular mitochondrial transfer alleviates pyroptosis in dental pulp damage. Cell Prolif 56:e13442 (2023). PubMed: 37086012
- Cai J et al. Antioxidant hepatic lipid metabolism can be promoted by orally administered inorganic nanoparticles. Nat Commun 14:3643 (2023). PubMed: 37339977
- Wu R et al. MicroRNA-577/EIF5A2 axis suppressed the proliferation of DDP-resistant nasopharyngeal carcinoma cells by blocking TGF-β signaling pathway. Chem Biol Drug Des 102:815-827 (2023). PubMed: 37500510