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小鼠单克隆抗体[hIgG1]抗人IgG H&L预吸附二抗(ab436)

Submit a review Q&A (3)References (1)

Key features and details

  • Mouse monoclonal [hIgG1] Anti-Human IgG H&L preadsorbed
  • Host species: Mouse
  • Isotype: IgG2a
  • Suitable for: ELISA, RIA

概述

  • 产品名称

    小鼠单克隆抗体[hIgG1]抗人IgG H&L预吸附二抗
    参阅全部 IgG 二抗

  • 宿主

    Mouse

  • 靶标种属

    Human

  • 特异性

    hIgG1 specifically recognizes human IgG. hIgG - 100% hIgG1 - 147% hIgG2 - 150% hIgG3 - 94% hIgG4 - 2.1% hIgM - 0.6% hIgA - 0.3% Goat IgG - <0.01% Rabbit IgG - <0.01% Horse IgG - <0.01% Pig IgG - <0.01% Cow IgG - <0.01% Sheep IgG - <0.01% Affinity Constant: 2x1010 L/mol human IgG.

  • 经测试应用

    适用于: ELISA, RIAmore details

  • 最小交叉反应


    Cow, Goat, Horse, Pig, Rabbit, Sheep more details

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 6.00
    Preservative: 0.095% Sodium azide
    Constituents: 0.335% PBS, 1.0878% Sodium citrate, 0.9% Sodium chloride
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    hIgG1

  • 同种型

    IgG2a

  • 轻链类型

    unknown

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab436于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ELISA
Use at an assay dependent dilution.
RIA
Use at an assay dependent dilution.
说明
ELISA
Use at an assay dependent dilution.
RIA
Use at an assay dependent dilution.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (1)

发表研究结果有使用 ab436？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab436 被引用在 1 文献中.

  • Deng YY  et al. Identification of the tumor-suppressive role of circular RNA-FOXO3 in colorectal cancer via regulation of miR-543/LATS1 axis. Oncol Rep 46:N/A (2021). PubMed: 34549306

客户评价及客户问答

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1-3 of 3 Abreviews or Q&A

Question

Hello,

I am curious if any of the mouse anti-human IgG Mabs listed below cross react with monkey IgG?

Mouse anti-Human IgG Fc secondary antibody (ab99757)

Mouse monoclonal [H2] SecondaryAntibody to Human IgG - Fc (ab99770)

Mouse anti-Human IgG1 Fc secondary antibody (ab1927)

Mouse anti-Human IgG heavy chain secondary antibody (ab9243)

Mouse monoclonal [hIgG1] SecondaryAntibody to Human IgG - H&L, pre-adsorbed (ab436)

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Answer

Thank you for contacting Abcam.

We have not tested any of those antibodies against monkey IgG, therefore I cannot say whether they will cross react or not. Due to this, you can take advantage of our testing discount program if you want, please see the link below for more details:

https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount



If there is anything else I can help you with, please let me know.

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Question

I am hunting for an antibody that will distinguish between intact human IgG and single F(ab). Does this monoclonal Distinguish between intact IgG antibody and the F(ab).

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Answer

It is unknown as to which constant region of the heavy chain this antibody recognises, so we do not know if this antibody could distinguish between the two.

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Question

Please advise if this antibody has cross reactivity with chimpanzee or not. Yours sincerely, Yoko Kurozumo

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Answer

We have not tested the cross reactivity of this antibody with chimpanzee or with any other monkey IgA or with other monkey Ig classes. Its cross reativity with human IgG is below 0.3% and with human IgM(plasma) 2.6%. Human IgM(Waldenström's macroglobulinemia) cross reacts less than 0.3%.

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