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小鼠单克隆抗体[5A9]抗人IgG Fc (HRP) (ab7499)

Submit a review Q&A (2)References (3)

Key features and details

  • Mouse monoclonal [5A9] Anti-Human IgG Fc (HRP)
  • Conjugation: HRP
  • Host species: Mouse
  • Isotype: IgG2a
  • Suitable for: Sandwich ELISA, ELISA

概述

  • 产品名称

    小鼠单克隆抗体[5A9]抗人IgG Fc (HRP)
    参阅全部 IgG 二抗

  • 宿主

    Mouse

  • 靶标种属

    Human

  • 经测试应用

    适用于: Sandwich ELISA, ELISAmore details

  • 免疫原

    These clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunised with IgG from human serum.

  • 偶联物

    HRP

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C.

  • 存储溶液

    pH: 7.40
    Preservative: 0.05% Proclin 300
    Constituents: 0.8% Sodium chloride, 0.02% Potassium chloride, 0.024% Monobasic dihydrogen potassium phosphate, 0.18% Dibasic monohydrogen sodium phosphate
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    5A9

  • 同种型

    IgG2a

  • 常规说明

    Concentration varies from lot to lot and can be provided on request.

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab7499于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Sandwich ELISA
Use at an assay dependent dilution.
ELISA
Use at an assay dependent dilution.
说明
Sandwich ELISA
Use at an assay dependent dilution.
ELISA
Use at an assay dependent dilution.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (3)

发表研究结果有使用 ab7499？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab7499 被引用在 3 文献中.

  • Cheng YJ  et al. The characters of antibodies against PLA2R in healthy individuals and in the patient with PLA2R associated membranous nephropathy. Eur J Med Res 28:128 (2023). PubMed: 36935517
  • Padula L  et al. Secreted heat shock protein gp96-Ig and OX40L-Fc combination vaccine enhances SARS-CoV-2 Spike (S) protein-specific B and T cell immune responses. Vaccine X 12:100202 (2022). PubMed: 35936992
  • Mboumba Bouassa RS  et al. Serum and cervicovaginal IgG immune responses against a7 and a9 HPV in non-vaccinated women at risk for cervical cancer: Implication for catch-up prophylactic HPV vaccination. PLoS One 15:e0233084 (2020). PubMed: 32421735

客户评价及客户问答

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1-2 of 2 Abreviews or Q&A

Question

This secondary is cross-reacting with denatured BSA. Previous lots have not been like this & FOCR also did the same.

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Answer

Thank you for calling Abcam earlier today.

I am sorry that the first replacement antibody we sent you did not prove to be successful and so as we discussed I am sending you a vial of ab99765. Your order number, for your records for this product is *-****.

If there is anything else I can help you with, please let me know.

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Question

This secondary is cross-reacting with denatured BSA. Previous lots have not been like this.

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Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement order.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

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