为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 定制化产品 & 合作关系
    定制化产品 & 合作关系

    定制化产品和商务合作加速您的诊断和治疗项目

    定制化产品

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

Immunology Immunoglobulins Heavy Chain IgG

小鼠单克隆抗体[2C11]抗人IgG1 Fc (ab1927)

  • Datasheet
  • SDS
Reviews (1)Q&A (5)References (12)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

运输费用

Promotion Information

Abpromise

产品质量保证,客户专家支持,

点击获取更多

ELISA - Mouse monoclonal [2C11] Anti-Human IgG1 Fc (ab1927)

    Key features and details

    • Mouse monoclonal [2C11] Anti-Human IgG1 Fc
    • Host species: Mouse
    • Isotype: IgG1
    • Suitable for: IHC-Fr, ELISA

    你可能会感兴趣

    Western blot
    Product image
    InstantBlue® Coomassie Protein Stain (ISB1L) (ab119211)
    偶联
    Product image
    Alexa Fluor® 488 Conjugation Kit (Fast) - Lightning-Link® (ab236553)
    封闭
    Normal Mouse Serum (sterile) (ab7486)

    查看更多相关产品

    概述

    • 产品名称

      小鼠单克隆抗体[2C11]抗人IgG1 Fc
      参阅全部 IgG1 二抗
    • 宿主

      Mouse
    • 靶标种属

      Human
    • 经测试应用

      适用于: IHC-Fr, ELISAmore details
    • 免疫原

      Full length native protein (purified) corresponding to Human IgG1.

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • 存储溶液

      pH: 7.40
      Preservative: 0.09% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • 纯度

      Protein A purified
    • 纯化说明

      Chromatography on protein A Sepharose
    • 克隆

      单克隆
    • 克隆编号

      2C11
    • 骨髓瘤

      Sp2/0
    • 同种型

      IgG1
    • 常规说明

      Concentration varies from lot to lot and can be provided on request.
    • 研究领域

      • Immunology
      • Immunoglobulins
      • Heavy Chain
      • IgG
      • Secondary antibodies
      • anti-Human
      • IgG
      • Unconjugated

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab1927于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IHC-Fr
    Use at an assay dependent concentration.
    ELISA
    Use at an assay dependent concentration. PubMed: 24373342
    说明
    IHC-Fr
    Use at an assay dependent concentration.
    ELISA
    Use at an assay dependent concentration. PubMed: 24373342

    图片

    • ELISA - Mouse monoclonal [2C11] Anti-Human IgG1 Fc (ab1927)
      ELISA - Mouse monoclonal [2C11] Anti-Human IgG1 Fc (ab1927)Marino et al PLoS One. 2017 May 18;12(5):e0177810. doi: 10.1371/journal.pone.0177810. eCollection 2017. Fig 5. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

      Characterisation of virus activity and functional expression of monoclonal antibodies.

      (Panel B) IgG1 antibody expression detected in cellular supernatants of HT-29 cells infected with 10ppc NG-135 by anti-IgG1 ELISA.

      Media was removed from infected or control cells, clarified by centrifugation and diluted 1 in 2 in 3% BSA/PBS for storage at -20°C. For quantification of human IgG1, 96 well plates (NUNC) were coated overnight, 4°C, with mouse monoclonal anti-human IgG1 Fc antibody (ab1927, Abcam) at a 1:1000 dilution in carbonate/bicarbonate buffer. Plates were blocked in 3% BSA/PBS, washed and samples and standards added to the plate for 1 hr and RT. Secondary detection was carried out using a HRP conjugated goat anti-human IgG Fab (ab87422, Abcam) incubated for 1 hr, RT.

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (12)

    发表研究结果有使用 ab1927?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab1927 被引用在 12 文献中.

    • Van Thillo Q  et al. Oncogenic cooperation between TCF7-SPI1 and NRAS(G12D) requires ß-catenin activity to drive T-cell acute lymphoblastic leukemia. Nat Commun 12:4164 (2021). PubMed: 34230493
    • Wang J  et al. Krüppel like factor 6 splice variant 1 (KLF6-SV1) overexpression recruits macrophages to participate in lung cancer metastasis by up-regulating TWIST1. Cancer Biol Ther 20:680-691 (2019). PubMed: 30590988
    • Zhao Z  et al. Upregulation of Insulin-Like Growth Factor-1 Receptor (IGF-1R) Reverses the Inhibitory Effect of Let-7g-5p on Migration and Invasion of Nasopharyngeal Carcinoma. Med Sci Monit 25:5747-5756 (2019). PubMed: 31374070
    • Wen Z  et al. Inhibition of lung cancer cells and Ras/Raf/MEK/ERK signal transduction by ectonucleoside triphosphate phosphohydrolase-7 (ENTPD7). Respir Res 20:194 (2019). PubMed: 31443651
    • Zhang Y  et al. Sirtuin 6 overexpression relieves sepsis-induced acute kidney injury by promoting autophagy. Cell Cycle 18:425-436 (2019). PubMed: 30700227
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-6 of 6 Abreviews or Q&A

    Mouse monoclonal 2C11 Anti-Human IgG1 Fc

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Growing cells on plates coated with Fc-DII4, differentiating to proT cells through staining with CD44 and CD25.
    Analyzed with FloCyt
    We have been using this antibody for years and have used 5 different batches.
    They have always done as promised in the datasheet.
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Apr 17 2017

    Question

    I am looking for a secondary antibody, which detects human IgG1 specifically without cross reactivity towards murine Fc. I would like to use such antibody in a FACS staining and the labeling of the antibody does not matter.
    Does your company provide such antibody. From the data sheet it is hard to judge because it always says that the antibody might cross react with other IgGs.
    I would be very happy if you could get back to me.

    Kind regards,

    Read More

    Abcam community

    Verified customer

    Asked on Jun 11 2012

    Answer

    Thank you for contacting us.

    ab1927 does not cross react with mouse IgGs so it could also be a option to try.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    回复于 Jun 11 2012

    Question

    I am looking for a secondary antibody, which detects human IgG1 specifically without cross reactivity towards murine Fc. I would like to use such antibody in a FACS staining and the labeling of the antibody does not matter.
    Does your company provide such antibody. From the data sheet it is hard to judge because it always says that the antibody might cross react with other IgGs.
    I would be very happy if you could get back to me.

    Kind regards,

    Read More

    Abcam community

    Verified customer

    Asked on Jun 08 2012

    Answer

    Thank you for contacting us.

    Antibody ab50473 does not react with mouse IgGs. This might be suitable as per your requirements.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    回复于 Jun 08 2012

    Question

    I am looking for a secondary antibody, which detects human IgG1 specifically without cross reactivity towards murine Fc. I would like to use such antibody in a FACS staining and the labeling of the antibody does not matter.
    Does your company provide such antibody. From the data sheet it is hard to judge because it always says that the antibody might cross react with other IgGs.
    I would be very happy if you could get back to me.

    Kind regards,

    Read More

    Abcam community

    Verified customer

    Asked on Jun 08 2012

    Answer

    Thank you for contacting us.

    It seems product ab1927 and ab50473, could be suitable however in order to confirm it I am now waiting, species cross reactivity data from lab. I will get back to you with more updates soon.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    回复于 Jun 08 2012

    Question

    Hello,

    I am curious if any of the mouse anti-human IgG Mabs listed below cross react with monkey IgG?

    Mouse anti-Human IgG Fc secondary antibody (ab99757)

    Mouse monoclonal [H2] SecondaryAntibody to Human IgG - Fc (ab99770)

    Mouse anti-Human IgG1 Fc secondary antibody (ab1927)

    Mouse anti-Human IgG heavy chain secondary antibody (ab9243)

    Mouse monoclonal [hIgG1] SecondaryAntibody to Human IgG - H&L, pre-adsorbed (ab436)

    Read More

    Abcam community

    Verified customer

    Asked on Mar 19 2012

    Answer

    Thank you for contacting Abcam.

    We have not tested any of those antibodies against monkey IgG, therefore I cannot say whether they will cross react or not. Due to this, you can take advantage of our testing discount program if you want, please see the link below for more details:

    https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount



    If there is anything else I can help you with, please let me know.

    Read More

    Abcam Scientific Support

    回复于 Mar 19 2012

    Question

    Is it IgG1, kappa or IgG1, lambda?

    Read More

    Abcam community

    Verified customer

    Asked on Apr 21 2005

    Answer

    Ab1927 - (Monoclonal mouse anti-human IgG1) - recognizes both kappa and lambda allotypes of human IgG1. Itself is isotype IgG1, and we have not determined if it is kappa or lambda allotype.

    Read More

    Abcam Scientific Support

    回复于 Apr 25 2005

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    通过邮件收到学术资源和促销信息 注册
    研究类别(A-Z)
    • 肿瘤研究
    • 心血管研究
    • 细胞生物学
    • 发育生物学
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 代谢
    • 微生物学
    • 神经生物学
    • 信号转导研究
    • 干细胞研究
    产品种类(A-Z)
    • 一抗
    • 二抗
    • 生化试剂
    • 亚类对照
    • 流式细胞仪多色选择器
    • 试剂盒
    • 内参对照
    • 细胞裂解液
    • 多肽
    • 蛋白酶
    • 组织切片
    • 蛋白标签及细胞标记物
    • 相关试剂及工具
    帮助与支持
    • 技术支持
    • 技术FAQ
    • 实验步骤与建议
    • 购买FAQ
    • RabMAb 产品
    • 培训课程
    • 按靶标浏览
    企业
    • 企业资讯
    • 投资者关系
    • 公司新闻
    • 招贤纳士
    • 关于我们
    活动通知
    • 商品展览
    • 会议
    全球网站
    • abcam.com
    • abcam.co.jp
    QR code for Weechat

    微信扫码 关注我们

    沪ICP备14046373号-1

    沪公网安备 31011502007413号

    • 销售条款
    • 网站使用条款
    • Cookie政策
    • 隐私权政策
    • 法律
    © 1998-2023 艾博抗(上海)贸易有限公司版权所有