小鼠单克隆抗体[2A 8F4]抗大鼠IgG2a heavy chain (Alexa Fluor® 488) (ab172332)
Key features and details
- Mouse monoclonal [2A 8F4] Anti-Rat IgG2a heavy chain (Alexa Fluor® 488)
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
- Host species: Mouse
- Isotype: IgG1
- Suitable for: IHC-Fr, Flow Cyt
概述
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产品名称
小鼠单克隆抗体[2A 8F4]抗大鼠IgG2a heavy chain (Alexa Fluor® 488)
参阅全部 IgG2a 二抗 -
宿主
Mouse -
靶标种属
Rat -
经测试应用
适用于: IHC-Fr, Flow Cytmore details -
免疫原
The details of the immunogen for this antibody are not available.
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偶联物
Alexa Fluor® 488. Ex: 495nm, Em: 519nm
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
存储溶液
Preservative: 0.02% Sodium azide
Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
单克隆 -
克隆编号
2A 8F4 -
同种型
IgG1 -
轻链类型
kappa -
常规说明
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab172332于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-Fr |
Use at an assay dependent concentration.
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Flow Cyt |
1/2000.
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说明 |
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IHC-Fr
Use at an assay dependent concentration. |
Flow Cyt
1/2000. |
图片
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Overlay histogram showing specificity of ab172332. HeLa cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the primary antibody as indicated (ab16881, ab81461, ab90458, 1μg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was Alexa Fluor® 488 mouse anti-rat IgG2a (heavy chain) at 1/2000 dilution for 30 min at 22ºC. Unlabelled (blue line) and secondary only (black line) samples were used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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Emission spectra of Alexa Fluor® fluorochromes available in our catalog. Line colors represent the approximate visible colors of the wavelength maxima.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab172332 被引用在 1 文献中.
- Harada T et al. Effects of recombinant human soluble thrombomodulin on neutrophil extracellular traps in the kidney of a mouse model of endotoxin shock. Fujita Med J 9:225-230 (2023). PubMed: 37554943