Anti-mouse IgG for IP (HRP)

• WB

Unknown

Western blot - Anti-mouse IgG for IP (HRP) (AB131368)

IP sample preparation : Caspase 7 was immunoprecipitated from 0.5 ml of 1x10⁷ Jurkat (Human T cell leukemia cell line from peripheral blood) cells/ml with 5 μg mouse anti-human Caspase 7.

WB conditions : Precipitate from 1x10⁶ cells was subjected to electrophoresis, transferred to an PVDF membrane, and immunoblotted with an anti-Caspase 7 antibody.

Detection :
Lane 1 : Detection with anti-mouse IgG for IP secondary antibody (HRP) (ab131368)
Lane 2 : Detection with an HRP-conjugated anti-mouse IgG H&L secondary antibody
Lane 3 : Lane 1 was re-immunoblotted using an HRP-conjugated anti-mouse IgG H&L secondary antibody. The heavy and light-chains can now be seen, confirming that although the immunoprecipitating heavy and light-chains are present, ab131368 detects only native antibody and not denatured heavy and light-chains.

Please note the detection of the heavy and light-chains of the immunoprecipitating antibody in Lane 2 but not in Lane 1.

Secondary

All lanes:

Western blot - Anti-mouse IgG for IP (HRP) (ab131368)

false

• IP

Supplier Data

Immunoprecipitation - Anti-mouse IgG for IP (HRP) (AB131368)

Histone H3 was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with ab309551 at 1/50 dilution (2ug in 0.4mg lysates). Western blot was performed on the immunoprecipitate using ab309551 at 1/1000 dilution. mouse IgG for IP (HRP) (ab131368) was used at 1/5000 dilution. Lane 1 : HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate Lane 2 : ab309551 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate Lane 3 : Mouse IgG1 monoclonal isotype control (ab18443) instead of ab309551 in HeLa whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 32 seconds

All lanes:

Immunoprecipitation - Anti-Histone H3 antibody [1B1B2] - Nuclear Marker (<a href='/products/primary-antibodies/histone-h3-antibody-1b1b2-nuclear-marker-ab309551'>ab309551</a>) at 1/50 dilution

All lanes:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - Anti-mouse IgG for IP (HRP) (ab131368) at 1/5000 dilution

false

Exposure time: 32s

• IP

Supplier Data

Immunoprecipitation - Anti-mouse IgG for IP (HRP) (AB131368)

RAB10 was immunoprecipitated from 0.35 mg A549 (human lung carcinoma epithelial cell) whole cell lysate 10 ug with ab307296 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307296 at 1/1000 dilution. Mouse IgG for IP (HRP) (ab131368) was used at 1/5000 dilution. Lane 1 : A549 (human lung carcinoma epithelial cell) whole cell lysate Lane 2 : ab307296 IP in A549 (human lung carcinoma epithelial cell) whole cell lysate Lane 3 : Mouse IgG2a Isotype control (ab18413) instead of ab307296 in A549 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 15 seconds.

All lanes:

Immunoprecipitation - Anti-RAB10 antibody [MJF-R23] - Mouse IgG2a (Chimeric) (<a href='/products/primary-antibodies/rab10-antibody-mjf-r23-mouse-igg2a-chimeric-ab307296'>ab307296</a>) at 1/30 dilution

All lanes:

A549 (human lung carcinoma epithelial cell) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - Anti-mouse IgG for IP (HRP) (ab131368) at 1/5000 dilution

false

Exposure time: 15s

• IP

Lab

Immunoprecipitation - Anti-mouse IgG for IP (HRP) (AB131368)

Viperin was immunoprecipitated from 0.35 mg KG-1a (human bone marrow myeloblast) whole cell lysate with ab107359 at 1/50 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab107359 at 1/1000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-Viperin antibody [MaP.VIP] (<a href='/products/primary-antibodies/viperin-antibody-mapvip-ab107359'>ab107359</a>) at 1/50 dilution

Lane 1:

KG-1a (human bone marrow myeloblast) whole cell lysate (Input) at 10 µg

Lane 2:

KG-1a (human bone marrow myeloblast) whole cell lysate (+)

Lane 3:

Mouse IgG2a, kappaIsotype control (<a href='/products/primary-antibodies/mouse-igg2a-kappa-monoclonal-mopc-173-isotype-control-chip-grade-ab18413'>ab18413</a>) instead of <a href='/products/primary-antibodies/viperin-antibody-mapvip-ab107359'>ab107359</a> in KG-1a whole cell lysate (-)

Secondary

All lanes:

Immunoprecipitation - Anti-mouse IgG for IP (HRP) (ab131368) at 1/5000 dilution

Observed band size: 42 kDa

false

Exposure time: 93s

• IP

Lab

Immunoprecipitation - Anti-mouse IgG for IP (HRP) (AB131368)

TATA binding protein TBP was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate (10 µg) with ab300656 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300656 at 1/2000 dilution. Mouse IgG for IP (HRP) (ab131368) was used as the secondary at 1/5000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-TATA binding protein TBP antibody [mAbcam51841] - Loading Control (<a href='/products/primary-antibodies/tata-binding-protein-tbp-antibody-mabcam51841-loading-control-ab300656'>ab300656</a>) at 1/30 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 10 µg

Lane 2:

HeLa whole cell lysate

Secondary

All lanes:

Immunoprecipitation - Anti-mouse IgG for IP (HRP) (ab131368) at 1/5000 dilution

Observed band size: 35 kDa,38 kDa

false

Exposure time: 24s

• IP

Supplier Data

Immunoprecipitation - Anti-mouse IgG for IP (HRP) (AB131368)

CP2c was immunoprecipitated from 0.35 mg K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate 10 ug with ab308611 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab308611 at 1/1000 dilution. mouse IgG for IP (HRP) (ab131368) was used at 1/1000 dilution. Lane 1 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate Lane 2 : ab308611 IP in K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate Lane 3 : Mouse monoclonal IgG (ab18443) instead of ab308611 in K-562 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 24 seconds

All lanes:

Immunoprecipitation - Anti-CP2c antibody [14/LSF] (<a href='/products/primary-antibodies/cp2c-antibody-14-lsf-ab308611'>ab308611</a>) at 1/30 dilution

All lanes:

K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - Anti-mouse IgG for IP (HRP) (ab131368) at 1/1000 dilution

false

Exposure time: 24s

• IP

Lab

Immunoprecipitation - Anti-mouse IgG for IP (HRP) (AB131368)

SOX10 was immunoprecipitated from 0.35 mg A375 (human malignant melanoma epithelial cell) whole cell lysate with ab218522 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab218522 at 1/1000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-SOX10 antibody [SOX10/991] (<a href='/products/primary-antibodies/sox10-antibody-sox10-991-ab218522'>ab218522</a>) at 1/30 dilution

Lane 1:

A375 (human malignant melanoma epithelial cell) whole cell lysate (Input) at 10 µg

Lane 2:

A375 whole cell lysate (+)

Lane 3:

Mouse IgG2b instead of <a href='/products/primary-antibodies/sox10-antibody-sox10-991-ab218522'>ab218522</a> in A375 whole cell lysate (-)

Secondary

All lanes:

Immunoprecipitation - Anti-mouse IgG for IP (HRP) (ab131368) at 1/1000 dilution

Observed band size: 56-75 kDa

false

Exposure time: 3s

• IP

Supplier Data

Immunoprecipitation - Anti-mouse IgG for IP (HRP) (AB131368)

p53 was immunoprecipitated from 0.35 mg HEK-293 (human embryonic kidney epithelial cell) whole cell lysate with ab308609 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab308609 at 1/1000 dilution. mouse IgG for IP (HRP) (ab131368) was used at 1/5000 dilution. Lane 1 : HEK-293 whole cell lysate Lane 2 : ab308609 IP in HEK-293 whole cell lysate Lane 3 : Mouse IgG1 monoclonal isotype control (ab18443) instead of ab308609 in HEK-293 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 23 seconds. The binding pattern observed is consistent with what has been described in the literature (PMID : 31045216; PMID : 16131611).

All lanes:

Immunoprecipitation - Anti-p53 antibody [G59-12] (<a href='/products/primary-antibodies/p53-antibody-g59-12-ab308609'>ab308609</a>) at 1/1000 dilution

All lanes:

HEK-293 whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - Anti-mouse IgG for IP (HRP) (ab131368) at 1/5000 dilution

false

• IP

Supplier Data

Immunoprecipitation - Anti-mouse IgG for IP (HRP) (AB131368)

RAB10 was immunoprecipitated from 0.35 mg C6 (rat glial tumor glial cell) whole cell lysate 10 ug with ab307296 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307296 at 1/1000 dilution. Mouse IgG for IP (HRP) (ab131368) was used at 1/5000 dilution. Lane 1 : C6 (rat glial tumor glial cell) whole cell lysate Lane 2 : ab307296 IP in C6 (rat glial tumor glial cell) whole cell lysate Lane 3 : Mouse IgG2a Isotype control (ab18413) instead of ab307296 in C6 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 15 seconds.

All lanes:

Immunoprecipitation - Anti-RAB10 antibody [MJF-R23] - Mouse IgG2a (Chimeric) (<a href='/products/primary-antibodies/rab10-antibody-mjf-r23-mouse-igg2a-chimeric-ab307296'>ab307296</a>) at 1/30 dilution

All lanes:

C6 (rat glial tumor glial cell) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - Anti-mouse IgG for IP (HRP) (ab131368) at 1/5000 dilution

false

Exposure time: 15s

• IP

Supplier Data

Immunoprecipitation - Anti-mouse IgG for IP (HRP) (AB131368)

Histone H3 was immunoprecipitated from 0.35 mg C6 (rat glial tumor glial cell) whole cell lysate with ab309551 at 1/50 dilution (2ug in 0.4mg lysates). Western blot was performed on the immunoprecipitate using ab309551 at 1/1000 dilution. mouse IgG for IP (HRP) (ab131368) was used at 1/5000 dilution. Lane 1 : C6 (rat glial tumor glial cell) whole cell lysate Lane 2 : ab309551 IP in C6 (rat glial tumor glial cell) whole cell lysate Lane 3 : Mouse IgG1 monoclonal isotype control (ab18443) instead of ab309551 in C6 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 67 seconds

All lanes:

Immunoprecipitation - Anti-Histone H3 antibody [1B1B2] - Nuclear Marker (<a href='/products/primary-antibodies/histone-h3-antibody-1b1b2-nuclear-marker-ab309551'>ab309551</a>) at 1/50 dilution

All lanes:

C6 (rat glial tumor glial cell) whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - Anti-mouse IgG for IP (HRP) (ab131368) at 1/5000 dilution

false

Exposure time: 67s

• IP

Lab

Immunoprecipitation - Anti-mouse IgG for IP (HRP) (AB131368)

TATA binding protein TBP was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate (10 µg) with ab300656 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300656 at 1/2000 dilution. Mouse IgG for IP (HRP) (ab131368) was used as the secondary at 1/5000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-TATA binding protein TBP antibody [mAbcam51841] - Loading Control (<a href='/products/primary-antibodies/tata-binding-protein-tbp-antibody-mabcam51841-loading-control-ab300656'>ab300656</a>) at 1/30 dilution

Lane 1:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 10 µg

Lane 2:

NIH/3T3 whole cell lysate

Secondary

All lanes:

Immunoprecipitation - Anti-mouse IgG for IP (HRP) (ab131368) at 1/5000 dilution

Predicted band size: 38 kDa

Observed band size: 36 kDa

false

Exposure time: 24s

• IP

Supplier Data

Immunoprecipitation - Anti-mouse IgG for IP (HRP) (AB131368)

RAB10 was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10 ug with ab307296 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307296 at 1/1000 dilution. Mouse IgG for IP (HRP) (ab131368) was used at 1/5000 dilution. Lane 1 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate Lane 2 : ab307296 IP in NIH/3T3 (mouse embryonic fibroblast) whole cell lysate Lane 3 : Mouse IgG2a Isotype control (ab18413) instead of ab307296 in NIH/3T3 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 15 seconds.

All lanes:

Immunoprecipitation - Anti-RAB10 antibody [MJF-R23] - Mouse IgG2a (Chimeric) (<a href='/products/primary-antibodies/rab10-antibody-mjf-r23-mouse-igg2a-chimeric-ab307296'>ab307296</a>) at 1/30 dilution

All lanes:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - Anti-mouse IgG for IP (HRP) (ab131368) at 1/5000 dilution

false

Exposure time: 15s

• IP

Supplier Data

Immunoprecipitation - Anti-mouse IgG for IP (HRP) (AB131368)

DCTN1/p150-glued was immunoprecipitated from 10μg of mouse brain tissue lysate with ab302966 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab302966 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used as secondary antibody at 1/5000 dilution. Lane 1 : Mouse brain tissue lysate 10μg (Input). Lane 2 : ab302966 IP in mouse brain tissue lysate. Lane 3 : Rabbit monoclonal IgG (ab18443) instead of ab302966 in mouse brain tissue lysate. Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 93 seconds.

All lanes:

Immunoprecipitation - Anti-DCTN1/p150-glued antibody [1/P150GLUED] (<a href='/products/primary-antibodies/dctn1-p150-glued-antibody-1-p150glued-ab302966'>ab302966</a>) at 1/30 dilution

All lanes:

Mouse brain tissue

Secondary

All lanes:

Immunoprecipitation - Anti-mouse IgG for IP (HRP) (ab131368) at 1/5000 dilution

Observed band size: 150 kDa

false

Exposure time: 93s

• IP

Lab

Immunoprecipitation - Anti-mouse IgG for IP (HRP) (AB131368)

Viperin was immunoprecipitated from 0.35 mg Mouse spleen tissue lysate with ab107359 at 1/50 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab107359 at 1/1000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-Viperin antibody [MaP.VIP] (<a href='/products/primary-antibodies/viperin-antibody-mapvip-ab107359'>ab107359</a>) at 1/50 dilution

Lane 1:

Mouse spleen tissue lysate (Input) at 10 µg

Lane 2:

Mouse spleen tissue lysate (+)

Lane 3:

Mouse IgG2a, kappaIsotype control (<a href='/products/primary-antibodies/mouse-igg2a-kappa-monoclonal-mopc-173-isotype-control-chip-grade-ab18413'>ab18413</a>) instead of <a href='/products/primary-antibodies/viperin-antibody-mapvip-ab107359'>ab107359</a> in KG-1a whole cell lysate (-)

Secondary

All lanes:

Immunoprecipitation - Anti-mouse IgG for IP (HRP) (ab131368) at 1/5000 dilution

Observed band size: 42 kDa

false

Exposure time: 93s

• IP

Supplier Data

Immunoprecipitation - Anti-mouse IgG for IP (HRP) (AB131368)

Histone H3 was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate with ab309551 at 1/50 dilution (2ug in 0.4mg lysates). Western blot was performed on the immunoprecipitate using ab309551 at 1/1000 dilution. mouse IgG for IP (HRP) (ab131368) was used at 1/5000 dilution. Lane 1 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate Lane 2 : ab309551 IP in NIH/3T3 (mouse embryonic fibroblast) whole cell lysate Lane 3 : Mouse IgG1 monoclonal isotype control (ab18443) instead of ab309551 in NIH/3T3 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 32 seconds

All lanes:

Immunoprecipitation - Anti-Histone H3 antibody [1B1B2] - Nuclear Marker (<a href='/products/primary-antibodies/histone-h3-antibody-1b1b2-nuclear-marker-ab309551'>ab309551</a>) at 1/50 dilution

All lanes:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - Anti-mouse IgG for IP (HRP) (ab131368) at 1/5000 dilution

false

Exposure time: 32s

• WB

Supplier Data

Western blot - Anti-mouse IgG for IP (HRP) (AB131368)

All lanes:

Western blot - Anti-Biotin antibody [Hyb-8] (<a href='/products/primary-antibodies/biotin-antibody-hyb-8-ab201341'>ab201341</a>) at 1/1000 dilution

Lane 1:

Human nerve lysate at 10 µg

Lane 2:

Human lower limb nerve lysate at 10 µg

Secondary

All lanes:

Western blot - Anti-mouse IgG for IP (HRP) (ab131368) at 1/1000 dilution

Observed band size: 58 kDa

false

Exposure time: 103s

• WB

Supplier Data

Western blot - Anti-mouse IgG for IP (HRP) (AB131368)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Exposure time : Lane 1 : 32 seconds; Lane 2 : 93 seconds

All lanes:

Western blot - Anti-Histone H3 antibody [1B1B2] - Nuclear Marker (<a href='/products/primary-antibodies/histone-h3-antibody-1b1b2-nuclear-marker-ab309551'>ab309551</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Anti-mouse IgG for IP (HRP) (ab131368) at 1/5000 dilution

Observed band size: 18 kDa

false

• WB

Unknown

Western blot - Anti-mouse IgG for IP (HRP) (AB131368)

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure times.

Lane 1 : 125 seconds; Lanes 2-5 : 26 seconds.

The molecular weight observed is consistent with what has been described in the literature (PMID : 15236336).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and azide (ab252540).

All lanes:

Western blot - Anti-CASK antibody [K56A/50] (<a href='/products/primary-antibodies/cask-antibody-k56a-50-ab252540'>ab252540</a>) at 1/1000 dilution

Lane 1:

Human brain tissue lysate at 20 µg

Lane 2:

Mouse brain tissue lysate at 20 µg

Lane 3:

Mouse cerebellum lysate at 20 µg

Lane 4:

Rat brain tissue lysate at 20 µg

Lane 5:

Rat cerebellum lysate at 20 µg

Secondary

Lane 1:

Western blot - Anti-mouse IgG for IP (HRP) (ab131368) at 1/100000 dilution

Lanes 2 - 5:

Peroxidase-Conjugated Goat anti-Mouse IgG（H+L） at 1/100000 dilution

Predicted band size: 105 kDa

Observed band size: 120 kDa

false

• WB

Lab

Western blot - Anti-mouse IgG for IP (HRP) (AB131368)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure times : Lane 1 : 3 seconds; Lane 2-4 : 1 second.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab231083).

All lanes:

Western blot - Anti-beta III Tubulin antibody [AA10] - Neuronal Marker (<a href='/products/primary-antibodies/beta-iii-tubulin-antibody-aa10-neuronal-marker-ab231083'>ab231083</a>) at 1/1000 dilution

Lane 1:

SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate at 10 µg

Lane 2:

Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate at 10 µg

Lane 3:

Mouse brain tissue lysate at 10 µg

Lane 4:

Rat brain tissue lysate at 10 µg

Secondary

Lane 1:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Lanes 2 - 4:

Western blot - Anti-mouse IgG for IP (HRP) (ab131368) at 1/1000 dilution

Predicted band size: 50 kDa

false

• WB

Supplier Data

Western blot - Anti-mouse IgG for IP (HRP) (AB131368)

Blocking and dilution buffer : 5% NFDM/TBST

Exposure time :

Lane 1 : 15 seconds

Lane 2 : 7.75 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab9519).

All lanes:

Western blot - Anti-Elastin antibody [BA-4] - BSA and Azide free (<a href='/products/primary-antibodies/elastin-antibody-ba-4-bsa-and-azide-free-ab269450'>ab269450</a>) at 0.51 µg/mL

Lane 1:

Human artery tissue lysate at 20 µg

Lane 2:

Human skin tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Anti-mouse IgG for IP (HRP) (ab131368) at 1/1000 dilution

Predicted band size: 68 kDa

Observed band size: 68 kDa

false

• WB

Supplier Data

Western blot - Anti-mouse IgG for IP (HRP) (AB131368)

This data was developed using ab11159 the same antibody clone in a different buffer formulation.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Myelin Basic Protein antibody [MBP2] - Oligodendrocyte Marker (<a href='/products/primary-antibodies/myelin-basic-protein-antibody-mbp2-oligodendrocyte-marker-ab11159'>ab11159</a>) at 1/1000 dilution

All lanes:

human brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Anti-mouse IgG for IP (HRP) (ab131368) at 1/50000 dilution

Predicted band size: 33 kDa

false

Exposure time: 26s

• WB

Lab

Western blot - Anti-mouse IgG for IP (HRP) (AB131368)

Blocking/Dilution buffer : 5% NFDM/TBST.

This image was produced using the same antibody clone but in a different formulation ab40, PBS, sodium azide, glycerol and BSA.

All lanes:

Western blot - Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] (<a href='/products/primary-antibodies/cytokeratin-5-6-18-antibody-lp34-ab40'>ab40</a>) at 1/1000 dilution

All lanes:

Human skin lysate at 20 µg

Secondary

All lanes:

Western blot - Anti-mouse IgG for IP (HRP) (ab131368) at 1/1000 dilution

Observed band size: 58 kDa

false

Exposure time: 114s

• WB

Supplier Data

Western blot - Anti-mouse IgG for IP (HRP) (AB131368)

FCN2 has a high molecular weight (400 kDa) due to extensive disulfide bridge formation (PMID : 16595153)

Negative control : Human brain (PMID : 8576206)

A weak unidentifiable band was observed in brain tissue

Blocking/diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-Ficolin 2 antibody [EPR23146-225] (<a href='/products/primary-antibodies/ficolin-2-antibody-epr23146-225-ab267473'>ab267473</a>) at 1/1000 dilution

Lane 1:

Human plasma at 20 µg

Lane 2:

Human liver tissue lysate at 20 µg

Lane 3:

Human brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Anti-mouse IgG for IP (HRP) (ab131368) at 1/1000 dilution

Predicted band size: 34 kDa

false

• WB

Supplier Data

Western blot - Anti-mouse IgG for IP (HRP) (AB131368)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure Time : Lane 1-3 : 15 seconds, lane 4-5 : 180 seconds.

All lanes:

Western blot - Anti-VAPB antibody [EPR27026-50] (<a href='/products/primary-antibodies/vapb-antibody-epr27026-50-ab315013'>ab315013</a>) at 1/1000 dilution

Lane 1:

Human testis tissue lysate at 20 µg

Lane 2:

Human liver tissue lysate at 20 µg

Lane 3:

Mouse testis tissue lysate at 20 µg

Lane 4:

Mouse liver tissue lysate at 20 µg

Lane 5:

Mouse colon tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Anti-mouse IgG for IP (HRP) (ab131368) at 1/5000 dilution

Observed band size: 27 kDa

false

• WB

Supplier Data

Western blot - Anti-mouse IgG for IP (HRP) (AB131368)

Blocking/Dilution buffer : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 24797388).

This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (ab252531).

All lanes:

Western blot - Anti-Properdin/PFC antibody [3A3E1] (<a href='/products/primary-antibodies/properdin-pfc-antibody-3a3e1-ab252531'>ab252531</a>) at 0.454 µg/mL

Lane 1:

Human plasma lysate at 20 µg

Lane 2:

Human serum lysate at 20 µg

Lane 3:

Mouse plasma lysate at 20 µg

Lane 4:

Mouse serum lysate at 20 µg

Lane 5:

Rat plasma lysate at 20 µg

Secondary

All lanes:

Western blot - Anti-mouse IgG for IP (HRP) (ab131368) at 1/1000 dilution

Predicted band size: 51 kDa

Observed band size: 55 kDa

false

Exposure time: 1s

• WB

Lab

Western blot - Anti-mouse IgG for IP (HRP) (AB131368)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-Human IgG antibody [IG266] (<a href='/products/primary-antibodies/human-igg-antibody-ig266-ab200699'>ab200699</a>) at 1/1000 dilution

Lane 1:

Human spleen tissue lysate at 10 µg

Lane 2:

Human tonsil tissue lysate at 10 µg

Secondary

All lanes:

Western blot - Anti-mouse IgG for IP (HRP) (ab131368) at 1/1000 dilution

Predicted band size: 36 kDa

Observed band size: 50 kDa

false

Exposure time: 6s

• WB

Lab

Western blot - Anti-mouse IgG for IP (HRP) (AB131368)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-Human IgG antibody [IG266] (<a href='/products/primary-antibodies/human-igg-antibody-ig266-ab200699'>ab200699</a>) at 1/5000 dilution

All lanes:

Human serum at 10 µg

Secondary

All lanes:

Western blot - Anti-mouse IgG for IP (HRP) (ab131368) at 1/1000 dilution

Predicted band size: 36 kDa

Observed band size: 50 kDa

false

Exposure time: 8s

• WB

Lab

Western blot - Anti-mouse IgG for IP (HRP) (AB131368)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure times : Lane 1, 2 : 3 seconds; Lane 3, 4 : 7 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254154).

All lanes:

Western blot - Anti-Detyrosinated alpha Tubulin antibody [AA12] - Loading Control (<a href='/products/primary-antibodies/detyrosinated-alpha-tubulin-antibody-aa12-loading-control-ab254154'>ab254154</a>) at 1/1000 dilution

Lane 1:

Human hippocampus tissue lysate at 20 µg

Lane 2:

Human brain tissue lysate at 20 µg

Lane 3:

Rat hippocampus tissue lysate at 20 µg

Lane 4:

Rat brain tissue lysate at 20 µg

Secondary

Lanes 1 - 2:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Lanes 3 - 4:

Western blot - Anti-mouse IgG for IP (HRP) (ab131368) at 1/1000 dilution

Predicted band size: 50 kDa

false

• WB

Supplier Data

Western blot - Anti-mouse IgG for IP (HRP) (AB131368)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.

All lanes:

Western blot - Anti-KACL antibody [EPR27403-26] (<a href='/products/primary-antibodies/kacl-antibody-epr27403-26-ab309102'>ab309102</a>) at 1/1000 dilution

All lanes:

Human skin tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Anti-mouse IgG for IP (HRP) (ab131368) at 1/1000 dilution

Observed band size: 32 kDa

true

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-mouse IgG for IP (HRP) (AB131368)

This data was developed using ab273441 the same antibody clone in a different buffer formulation.

Negative control : liver (PMID : 2120242).

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure times : Lane 1-2 : 10 seconds; Lane 3-5 : 37 seconds.

All lanes:

Western blot - Anti-68kDa Neurofilament/NF-L antibody [Nfl21] (<a href='/products/primary-antibodies/68kda-neurofilament-nf-l-antibody-nfl21-ab273441'>ab273441</a>) at 1/1000 dilution

Lane 1:

Mouse hippocampus tissue lysate at 20 µg

Lane 2:

Mouse liver tissue lysate at 20 µg

Lane 3:

Rat brain tissue lysate at 20 µg

Lane 4:

Rat hippocampus tissue lysate at 20 µg

Lane 5:

Rat liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Anti-mouse IgG for IP (HRP) (ab131368) at 1/1000 dilution

Predicted band size: 62 kDa

false

• WB

Supplier Data

Western blot - Anti-mouse IgG for IP (HRP) (AB131368)

This data was developed using ab273442 the same antibody clone in a different buffer formulation.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-68kDa Neurofilament/NF-L antibody [NfL23] (<a href='/products/primary-antibodies/68kda-neurofilament-nf-l-antibody-nfl23-ab273442'>ab273442</a>) at 1/1000 dilution

Lane 1:

Mouse hippocampus tissue lysate at 20 µg

Lane 2:

Mouse liver tissue lysate at 20 µg

Lane 3:

Rat brain tissue lysate at 20 µg

Lane 4:

Rat hippocampus tissue lysate at 20 µg

Lane 5:

Rat liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Anti-mouse IgG for IP (HRP) (ab131368) at 1/1000 dilution

Predicted band size: 62 kDa

Observed band size: 68 kDa

false

• WB

Lab

Western blot - Anti-mouse IgG for IP (HRP) (AB131368)

Blocking/Dilution buffer : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab28035).

All lanes:

Western blot - Anti-beta II Tubulin antibody [7B9] (<a href='/products/primary-antibodies/beta-ii-tubulin-antibody-7b9-ab28035'>ab28035</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Anti-mouse IgG for IP (HRP) (ab131368) at 1/1000 dilution

Predicted band size: 50 kDa

false

Exposure time: 1s

• WB

Lab

Western blot - Anti-mouse IgG for IP (HRP) (AB131368)

Blocking/Dilution buffer : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab28035).

All lanes:

Western blot - Anti-beta II Tubulin antibody [7B9] (<a href='/products/primary-antibodies/beta-ii-tubulin-antibody-7b9-ab28035'>ab28035</a>) at 1/1000 dilution

Lane 1:

C6 (rat glial tumor glial cell), whole cell lysate at 20 µg

Lane 2:

PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Anti-mouse IgG for IP (HRP) (ab131368) at 1/1000 dilution

Predicted band size: 50 kDa

false

• WB

Unknown

Western blot - Anti-mouse IgG for IP (HRP) (AB131368)

Blocking/Dilution buffer : 5% NFDM/TBST.

Negative control : Mouse heart (PMID : 15899979).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254154).

All lanes:

Western blot - Anti-Detyrosinated alpha Tubulin antibody [AA12] - Loading Control (<a href='/products/primary-antibodies/detyrosinated-alpha-tubulin-antibody-aa12-loading-control-ab254154'>ab254154</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse heart tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Anti-mouse IgG for IP (HRP) (ab131368) at 1/1000 dilution

Predicted band size: 50 kDa

false

Exposure time: 7s

• WB

Supplier Data

Western blot - Anti-mouse IgG for IP (HRP) (AB131368)

All lanes:

Western blot - Anti-Biotin antibody [Hyb-8] (<a href='/products/primary-antibodies/biotin-antibody-hyb-8-ab201341'>ab201341</a>) at 1/1000 dilution

Lane 1:

Mouse eyeball tissue lysate at 10 µg

Lane 2:

Rat eyeball tissue lysate at 10 µg

Lane 3:

Mouse C57 P20 retina at 10 µg

Secondary

All lanes:

Western blot - Anti-mouse IgG for IP (HRP) (ab131368)

Observed band size: 36 kDa

false

Exposure time: 3min

• IP

Supplier Data

Immunoprecipitation - Anti-mouse IgG for IP (HRP) (AB131368)

Influenza A virus PB1 protein was immunoprecipitated from 0.35 mg 293T cells transfected with a influenza A virus (H3N2) PB1 expression vector containing a myc-his-tag whole cell lysate with ab324448 at 1/50 dilution (2 ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab324448 at 1/1000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-Influenza A virus PB1 protein antibody [F5-19] (<a href='/products/primary-antibodies/influenza-a-virus-pb1-protein-antibody-f5-19-ab324448'>ab324448</a>) at 1/1000 dilution

Lane 1:

(Input) 293T cells transfected with a influenza A virus (H3N2) PB1 expression vector containing a myc-his-tag whole cell lysate at 10 µg

Lane 2:

<a href='/products/primary-antibodies/influenza-a-virus-pb1-protein-antibody-f5-19-ab324448'>ab324448</a> at 1/50 IP in 293T cells transfected with a influenza A virus (H3N2) PB1 expression vector containing a myc-his-tag whole cell lysate at 10 µg

Lane 3:

Mouse IgG1, kappa monoclonal [MOPC-21] - isotype control (<a href='/products/primary-antibodies/mouse-igg1-kappa-monoclonal-mopc-21-isotype-control-ab18443'>ab18443</a>) instead of <a href='/products/primary-antibodies/influenza-a-virus-pb1-protein-antibody-f5-19-ab324448'>ab324448</a> in 293T cells transfected with a influenza A virus (H3N2) PB1 expression vector containing a myc-his-tag whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - Anti-mouse IgG for IP (HRP) (ab131368) at 1/5000 dilution

Observed band size: 85 kDa

false

Exposure time: 8s

• WB

Unknown

Western blot - Anti-mouse IgG for IP (HRP) (AB131368)

All lanes:

Western blot - Anti-Estrogen Receptor alpha antibody [CRET94D] (<a href='/products/primary-antibodies/estrogen-receptor-alpha-antibody-cret94d-ab241557'>ab241557</a>) at 1/1000 dilution

Lane 1:

Human breast cancer tissue lysates at 20 µg

Lane 2:

Mouse breast tissue lysates at 20 µg

Lane 3:

Mouse ovary tissue lysates at 20 µg

Secondary

All lanes:

Western blot - Anti-mouse IgG for IP (HRP) (ab131368)

Predicted band size: 66 kDa

Observed band size: 66 kDa

false